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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              20     509
NP_001263689                                                           20     509
NP_001119584                                                           20     509
NP_001119586                                                           20     509
NP_001263625                                                           20     509
NP_001119590                                                           20     509
NP_001263690                                                           20     509
NP_001119585                                                           20     509
NP_001263624                                                           20     509
NP_001119588                                                           20     509
NP_001119587                                                           20     509
NP_001119589                                                           20     509
NP_665806                                                              18    1482
XP_006723074                                                           18    1482
NP_001263627                                                           18    1935
NP_001263628                                                           18    1935
NP_001263626                                                           18    1935
NP_003334                                                              16    7355
NP_001243621                                                           15    9605
NP_001243618                                                           15    9605
NP_001243614                                                           15    9605
NP_001243620                                                           15    9605
NP_001243617                                                           15    9605
NP_055606                                                              15    9605
NP_001243619                                                           15    9605
NP_001243622                                                           15    9605
NP_001243616                                                           15    9605
NP_064506                                                              15    9605
NP_038464                                                              15    9605
NP_995314                                                              15    9605
XP_006712264                                                           15    9605
XP_006712263                                                           15    9605

>NP_000537
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVMAIYKQS 9
           R MAIYKQS
Sbjct: 158 RAMAIYKQS 166



>NP_001263689
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVMAIYKQS 9
           R MAIYKQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119584
          Length = 393

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVMAIYKQS 9
           R MAIYKQS
Sbjct: 158 RAMAIYKQS 166



>NP_001119586
          Length = 341

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVMAIYKQS 9
           R MAIYKQS
Sbjct: 158 RAMAIYKQS 166



>NP_001263625
          Length = 302

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVMAIYKQS 9
           R MAIYKQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119590
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVMAIYKQS 9
           R MAIYKQS
Sbjct: 119 RAMAIYKQS 127



>NP_001263690
          Length = 354

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVMAIYKQS 9
           R MAIYKQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119585
          Length = 346

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVMAIYKQS 9
           R MAIYKQS
Sbjct: 158 RAMAIYKQS 166



>NP_001263624
          Length = 307

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVMAIYKQS 9
           R MAIYKQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119588
          Length = 209

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RVMAIYKQS 9
          R MAIYKQS
Sbjct: 26 RAMAIYKQS 34



>NP_001119587
          Length = 261

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RVMAIYKQS 9
          R MAIYKQS
Sbjct: 26 RAMAIYKQS 34



>NP_001119589
          Length = 214

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RVMAIYKQS 9
          R MAIYKQS
Sbjct: 26 RAMAIYKQS 34



>NP_665806
          Length = 489

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RVMAIYKQ 8
           RV+A+YKQ
Sbjct: 390 RVLAVYKQ 397



>XP_006723074
          Length = 489

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RVMAIYKQ 8
           RV+A+YKQ
Sbjct: 390 RVLAVYKQ 397



>NP_001263627
          Length = 182

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3 MAIYKQS 9
         MAIYKQS
Sbjct: 1 MAIYKQS 7



>NP_001263628
          Length = 187

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3 MAIYKQS 9
         MAIYKQS
Sbjct: 1 MAIYKQS 7



>NP_001263626
          Length = 234

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3 MAIYKQS 9
         MAIYKQS
Sbjct: 1 MAIYKQS 7



>NP_003334
          Length = 165

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RVMAIYKQ 8
          R+MA YKQ
Sbjct: 8  RLMAEYKQ 15



>NP_001243621
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  VMAIYKQ 8
          VMA+YK+
Sbjct: 83 VMALYKK 89



>NP_001243618
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VMAIYKQ 8
           VMA+YK+
Sbjct: 182 VMALYKK 188



>NP_001243614
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VMAIYKQ 8
           VMA+YK+
Sbjct: 214 VMALYKK 220



>NP_001243620
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VMAIYKQ 8
           VMA+YK+
Sbjct: 134 VMALYKK 140



>NP_001243617
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VMAIYKQ 8
           VMA+YK+
Sbjct: 143 VMALYKK 149



>NP_055606
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VMAIYKQ 8
           VMA+YK+
Sbjct: 214 VMALYKK 220



>NP_001243619
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VMAIYKQ 8
           VMA+YK+
Sbjct: 143 VMALYKK 149



>NP_001243622
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  VMAIYKQ 8
          VMA+YK+
Sbjct: 20 VMALYKK 26



>NP_001243616
          Length = 603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VMAIYKQ 8
           VMA+YK+
Sbjct: 166 VMALYKK 172



>NP_064506
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  MAIYKQS 9
          +AIYKQ+
Sbjct: 74 LAIYKQT 80



>NP_038464
          Length = 1128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1118 AVYKQS 1123



>NP_995314
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1124 AVYKQS 1129



>XP_006712264
          Length = 1126

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1116 AVYKQS 1121



>XP_006712263
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1122 AVYKQS 1127



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.129    0.327 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 318,439
Number of extensions: 203
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)