Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001078518                                                           18    1135
NP_036351                                                              18    1135
NP_789765                                                              18    1135
NP_001123914                                                           18    1135
NP_005334                                                              18    1135
NP_004976                                                              18    1135
NP_203524                                                              18    1135
NP_001239019                                                           18    1135
NP_002515                                                              18    1135
NP_853510                                                              18    1135
NP_036382                                                              18    1135
NP_006261                                                              18    1135
XP_006725199                                                           18    1135
XP_006719132                                                           18    1135
XP_006718280                                                           18    1135
XP_005247286                                                           18    1135
XP_005247285                                                           18    1135
NP_002877                                                              18    1482
NP_001238851                                                           18    1482
NP_056461                                                              18    1482
NP_001238850                                                           18    1482
NP_001010942                                                           18    1482
NP_067006                                                              18    1482
NP_001258115                                                           18    1482
XP_006710866                                                           18    1482
NP_066361                                                              18    1482
NP_001010935                                                           18    1482
NP_002875                                                              18    1482
NP_005393                                                              18    1935
NP_002872                                                              18    1935
XP_006715826                                                           18    1935
XP_006715825                                                           18    1935
XP_005263786                                                           18    1935
XP_005263785                                                           18    1935
XP_005263784                                                           18    1935
XP_005263781                                                           18    1935
NP_005605                                                              17    2528
NP_996563                                                              17    3301
NP_001273130                                                           17    4312
NP_001243750                                                           17    4312
NP_008843                                                              17    4312
NP_001259006                                                           17    4312
NP_002921                                                              17    4312
NP_057647                                                              17    4312
XP_005254491                                                           17    4312
NP_116307                                                              16    5631
XP_006716144                                                           16    5631
NP_997464                                                              16    7355
NP_001244067                                                           15    9605
NP_113671                                                              15    9605
NP_001244066                                                           15    9605
XP_005260627                                                           15    9605
XP_006723945                                                           15    9605
XP_006723944                                                           15    9605
>NP_001078518
          Length = 208

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 24 VGKSALTI 31
>NP_036351
          Length = 208

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 24 VGKSALTI 31
>NP_789765
          Length = 170

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 14 VGKSALTI 21
>NP_001123914
          Length = 189

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 14 VGKSALTI 21
>NP_005334
          Length = 189

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 14 VGKSALTI 21
>NP_004976
          Length = 188

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 14 VGKSALTI 21
>NP_203524
          Length = 189

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 14 VGKSALTI 21
>NP_001239019
          Length = 208

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 24 VGKSALTI 31
>NP_002515
          Length = 189

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 14 VGKSALTI 21
>NP_853510
          Length = 233

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 52 VGKSALTI 59
>NP_036382
          Length = 204

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 25 VGKSALTI 32
>NP_006261
          Length = 218

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 40 VGKSALTI 47
>XP_006725199
          Length = 170

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 14 VGKSALTI 21
>XP_006719132
          Length = 189

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 14 VGKSALTI 21
>XP_006718280
          Length = 170

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 14 VGKSALTI 21
>XP_005247286
          Length = 170

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 24 VGKSALTI 31
>XP_005247285
          Length = 208

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALTI
Sbjct: 24 VGKSALTI 31
>NP_002877
          Length = 183

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 14 VGKSALTV 21
>NP_001238851
          Length = 137

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 14 VGKSALTV 21
>NP_056461
          Length = 184

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 14 VGKSALTV 21
>NP_001238850
          Length = 165

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 14 VGKSALTV 21
>NP_001010942
          Length = 184

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 14 VGKSALTV 21
>NP_067006
          Length = 183

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 14 VGKSALTV 21
>NP_001258115
          Length = 183

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 14 VGKSALTV 21
>XP_006710866
          Length = 184

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 14 VGKSALTV 21
>NP_066361
          Length = 183

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 14 VGKSALTV 21
>NP_001010935
          Length = 184

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 14 VGKSALTV 21
>NP_002875
          Length = 184

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 14 VGKSALTV 21
>NP_005393
          Length = 206

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 25 VGKSALTL 32
>NP_002872
          Length = 206

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 25 VGKSALTL 32
>XP_006715826
          Length = 206

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 25 VGKSALTL 32
>XP_006715825
          Length = 206

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 25 VGKSALTL 32
>XP_005263786
          Length = 206

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 25 VGKSALTL 32
>XP_005263785
          Length = 206

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 25 VGKSALTL 32
>XP_005263784
          Length = 206

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 25 VGKSALTL 32
>XP_005263781
          Length = 228

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSALT+
Sbjct: 47 VGKSALTL 54
>NP_005605
          Length = 184

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKS+LTI
Sbjct: 17 VGKSSLTI 24
>NP_996563
          Length = 242

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RVGKSALTI 9
          RVGKSA+ +
Sbjct: 37 RVGKSAMIV 45
>NP_001273130
          Length = 204

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  GKSALTI 9
          GKSALT+
Sbjct: 15 GKSALTV 21
>NP_001243750
          Length = 236

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSA+T+
Sbjct: 49 VGKSAMTM 56
>NP_008843
          Length = 219

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSA+T+
Sbjct: 32 VGKSAMTM 39
>NP_001259006
          Length = 153

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSA+T+
Sbjct: 31 VGKSAMTM 38
>NP_002921
          Length = 217

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          VGKSA+T+
Sbjct: 31 VGKSAMTM 38
>NP_057647
          Length = 266

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  GKSALTI 9
          GKSALT+
Sbjct: 32 GKSALTV 38
>XP_005254491
          Length = 151

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  GKSALTI 9
          GKSALT+
Sbjct: 32 GKSALTV 38
>NP_116307
          Length = 199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VGKSALTI 9
          VGKSAL +
Sbjct: 17 VGKSALVV 24
>XP_006716144
          Length = 173

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  VGKSALTI 9
          +GKS+LTI
Sbjct: 6  LGKSSLTI 13
>NP_997464
          Length = 320

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RVGKSALT 8
           R+G SALT
Sbjct: 236 RLGSSALT 243
>NP_001244067
          Length = 752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RVGKSAL 7
           R GKSAL
Sbjct: 359 RTGKSAL 365
>NP_113671
          Length = 862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RVGKSAL 7
           R GKSAL
Sbjct: 469 RTGKSAL 475
>NP_001244066
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RVGKSAL 7
           R GKSAL
Sbjct: 510 RTGKSAL 516
>XP_005260627
          Length = 692

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RVGKSAL 7
           R GKSAL
Sbjct: 299 RTGKSAL 305
>XP_006723945
          Length = 752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RVGKSAL 7
           R GKSAL
Sbjct: 359 RTGKSAL 365
>XP_006723944
          Length = 862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RVGKSAL 7
           R GKSAL
Sbjct: 469 RTGKSAL 475
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.132    0.330 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 399,620
Number of extensions: 542
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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