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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              20     390
NP_001128581                                                           17    4312
NP_078833                                                              17    4312
NP_001193600                                                           17    4312
NP_001193601                                                           17    4312
NP_071941                                                              17    4312
XP_006718383                                                           17    4312
XP_006718382                                                           17    4312
XP_006718381                                                           17    4312
XP_006718380                                                           17    4312
XP_006718379                                                           17    4312
NP_001207417                                                           16    5631
XP_006721183                                                           16    5631
NP_001248                                                              16    5631
NP_001207419                                                           16    5631
NP_001207418                                                           16    5631
NP_001073936                                                           16    7355
NP_003092                                                              15    9605
NP_001239441                                                           15    9605
NP_001239440                                                           15    9605

>NP_004295
          Length = 1620

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RTSTIMIDY 9
            RTSTIM DY
Sbjct: 1084 RTSTIMTDY 1092



>NP_001128581
          Length = 187

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RTSTIMIDY 9
          R  TI++DY
Sbjct: 60 RQETILVDY 68



>NP_078833
          Length = 368

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTSTIMIDY 9
           R  TI++DY
Sbjct: 241 RQETILVDY 249



>NP_001193600
          Length = 369

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTSTIMIDY 9
           R  TI++DY
Sbjct: 242 RQETILVDY 250



>NP_001193601
          Length = 288

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTSTIMIDY 9
           R  TI++DY
Sbjct: 161 RQETILVDY 169



>NP_071941
          Length = 396

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   STIMIDY 9
           +T+MIDY
Sbjct: 230 NTVMIDY 236



>XP_006718383
          Length = 207

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RTSTIMIDY 9
          R  TI++DY
Sbjct: 80 RQETILVDY 88



>XP_006718382
          Length = 261

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTSTIMIDY 9
           R  TI++DY
Sbjct: 134 RQETILVDY 142



>XP_006718381
          Length = 368

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTSTIMIDY 9
           R  TI++DY
Sbjct: 241 RQETILVDY 249



>XP_006718380
          Length = 397

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTSTIMIDY 9
           R  TI++DY
Sbjct: 270 RQETILVDY 278



>XP_006718379
          Length = 397

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTSTIMIDY 9
           R  TI++DY
Sbjct: 270 RQETILVDY 278



>NP_001207417
          Length = 760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TSTIMID 8
           T+TIMID
Sbjct: 395 TATIMID 401



>XP_006721183
          Length = 422

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TSTIMID 8
           T+TIMID
Sbjct: 348 TATIMID 354



>NP_001248
          Length = 713

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TSTIMID 8
           T+TIMID
Sbjct: 348 TATIMID 354



>NP_001207419
          Length = 459

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TSTIMID 8
           T+TIMID
Sbjct: 94  TATIMID 100



>NP_001207418
          Length = 674

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TSTIMID 8
           T+TIMID
Sbjct: 309 TATIMID 315



>NP_001073936
          Length = 1848

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RTSTIMI 7
           RT+TIMI
Sbjct: 765 RTATIMI 771



>NP_003092
          Length = 550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   STIMIDY 9
           ST+++DY
Sbjct: 155 STLVVDY 161



>NP_001239441
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  STIMIDY 9
          ST+++DY
Sbjct: 90 STLVVDY 96



>NP_001239440
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   STIMIDY 9
           ST+++DY
Sbjct: 97  STLVVDY 103



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.135    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 299,723
Number of extensions: 480
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)