Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           20     509
NP_000170                                                              20     509
NP_001268422                                                           20     509
NP_001268423                                                           20     509
XP_005264328                                                           20     509
NP_009068                                                              17    4312
NP_076971                                                              17    4312
XP_005272657                                                           17    4312
XP_005271245                                                           17    4312
XP_005271244                                                           17    4312
XP_005271243                                                           17    4312
XP_005271241                                                           17    4312
XP_005259280                                                           16    5631
XP_005259279                                                           16    5631
NP_037494                                                              16    5631
NP_001274103                                                           16    7355
NP_000343                                                              16    7355
NP_055959                                                              16    7355
XP_005255915                                                           16    7355
XP_006718349                                                           16    7355
NP_002431                                                              15    9605
XP_005257785                                                           15    9605
NP_115510                                                              15    9605
>NP_001268421
          Length = 1230

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RTMSGESTF 9
            R MSGESTF
Sbjct: 1052 RIMSGESTF 1060
>NP_000170
          Length = 1360

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RTMSGESTF 9
            R MSGESTF
Sbjct: 1182 RIMSGESTF 1190
>NP_001268422
          Length = 1058

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RTMSGESTF 9
           R MSGESTF
Sbjct: 880 RIMSGESTF 888
>NP_001268423
          Length = 1058

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RTMSGESTF 9
           R MSGESTF
Sbjct: 880 RIMSGESTF 888
>XP_005264328
          Length = 1261

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RTMSGESTF 9
            R MSGESTF
Sbjct: 1083 RIMSGESTF 1091
>NP_009068
          Length = 661

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RTMSGESTF 9
           R +SG+STF
Sbjct: 103 RRISGKSTF 111
>NP_076971
          Length = 337

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RTMSGESTF 9
           RTM  ESTF
Sbjct: 176 RTMEIESTF 184
>XP_005272657
          Length = 661

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RTMSGESTF 9
           R +SG+STF
Sbjct: 103 RRISGKSTF 111
>XP_005271245
          Length = 259

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RTMSGESTF 9
           RTM  ESTF
Sbjct: 98  RTMEIESTF 106
>XP_005271244
          Length = 259

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RTMSGESTF 9
           RTM  ESTF
Sbjct: 98  RTMEIESTF 106
>XP_005271243
          Length = 286

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RTMSGESTF 9
           RTM  ESTF
Sbjct: 176 RTMEIESTF 184
>XP_005271241
          Length = 314

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RTMSGESTF 9
           RTM  ESTF
Sbjct: 153 RTMEIESTF 161
>XP_005259280
          Length = 623

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RTMSGESTF 9
           R  SGE+TF
Sbjct: 309 RVHSGETTF 317
>XP_005259279
          Length = 706

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RTMSGESTF 9
           R  SGE+TF
Sbjct: 392 RVHSGETTF 400
>NP_037494
          Length = 706

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RTMSGESTF 9
           R  SGE+TF
Sbjct: 392 RVHSGETTF 400
>NP_001274103
          Length = 1582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RTMSGESTF 9
            RT SG+S+F
Sbjct: 1380 RTGSGKSSF 1388
>NP_000343
          Length = 1581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RTMSGESTF 9
            RT SG+S+F
Sbjct: 1379 RTGSGKSSF 1387
>NP_055959
          Length = 949

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSGESTF 9
           +SG+STF
Sbjct: 864 LSGQSTF 870
>XP_005255915
          Length = 1099

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    MSGESTF 9
            +SG+STF
Sbjct: 1001 LSGQSTF 1007
>XP_006718349
          Length = 1646

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RTMSGESTF 9
            RT SG+S+F
Sbjct: 1379 RTGSGKSSF 1387
>NP_002431
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MSGESTF 9
           MSG+ST+
Sbjct: 683 MSGKSTY 689
>XP_005257785
          Length = 1887

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TMSGEST 8
           T+SG+ST
Sbjct: 258 TLSGDST 264
>NP_115510
          Length = 1663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  TMSGEST 8
          T+SG+ST
Sbjct: 92 TLSGDST 98
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.117    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 592,011
Number of extensions: 940
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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