Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              20     390
NP_115791                                                              17    3301
NP_005040                                                              17    3301
XP_006723973                                                           17    3301
XP_006715859                                                           17    3301
XP_006715858                                                           17    3301
XP_006715857                                                           17    3301
XP_006710159                                                           17    3301
NP_001254635                                                           17    4312
NP_054862                                                              17    4312
NP_006161                                                              17    4312
NP_001028886                                                           17    4312
NP_001245239                                                           17    4312
NP_001245237                                                           17    4312
NP_001245238                                                           17    4312
XP_005253748                                                           17    4312
NP_055999                                                              16    5631
NP_001137854                                                           16    5631
NP_001160006                                                           16    5631
NP_001160005                                                           16    5631
XP_005248604                                                           16    5631
XP_005248602                                                           16    5631
XP_006713136                                                           16    5631
NP_001136021                                                           16    5631
NP_077726                                                              16    5631
NP_001191330                                                           16    7355
NP_001158                                                              16    7355
NP_006827                                                              16    7355
XP_006724817                                                           16    7355
XP_005269369                                                           16    7355
XP_005269368                                                           16    7355
XP_005269367                                                           16    7355
NP_689912                                                              16    7355
NP_001124295                                                           15    9605
NP_001370                                                              15    9605
NP_001166037                                                           15    9605
NP_001166039                                                           15    9605
NP_002459                                                              15    9605
NP_001166040                                                           15    9605
NP_001166038                                                           15    9605
NP_036441                                                              15    9605
NP_001277114                                                           15    9605
NP_068375                                                              15    9605
NP_573573                                                              15    9605
NP_061836                                                              15    9605
NP_079175                                                              15    9605
NP_001258918                                                           15    9605
NP_057306                                                              15    9605
NP_387505                                                              15    9605
XP_005261051                                                           15    9605
XP_006722744                                                           15    9605
XP_006722420                                                           15    9605
XP_006722419                                                           15    9605
XP_005258207                                                           15    9605
XP_005258206                                                           15    9605
XP_005277989                                                           15    9605
XP_006718541                                                           15    9605
XP_006718540                                                           15    9605
XP_005277987                                                           15    9605
XP_006714933                                                           15    9605
XP_006713233                                                           15    9605
XP_005265229                                                           15    9605
XP_005245315                                                           15    9605
XP_006711447                                                           15    9605
XP_005245314                                                           15    9605
NP_002637                                                              15    9605
>NP_055365
          Length = 1032

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  RTGRLMDI 8
          RTGRLMDI
Sbjct: 77 RTGRLMDI 84
>NP_115791
          Length = 1154

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RTGRLMDI 8
          R GRL+DI
Sbjct: 72 RAGRLLDI 79
>NP_005040
          Length = 919

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RTGRLMDI 8
           +TGRL+D+
Sbjct: 489 QTGRLLDV 496
>XP_006723973
          Length = 919

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RTGRLMDI 8
           +TGRL+D+
Sbjct: 489 QTGRLLDV 496
>XP_006715859
          Length = 854

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RTGRLMDI 8
          R GRL+DI
Sbjct: 72 RAGRLLDI 79
>XP_006715858
          Length = 1267

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RTGRLMDI 8
          R GRL+DI
Sbjct: 72 RAGRLLDI 79
>XP_006715857
          Length = 1268

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RTGRLMDI 8
          R GRL+DI
Sbjct: 72 RAGRLLDI 79
>XP_006710159
          Length = 919

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RTGRLMDI 8
           +TGRL+D+
Sbjct: 489 QTGRLLDV 496
>NP_001254635
          Length = 176

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RTGRLMDI 8
           R GR+MD+
Sbjct: 148 RKGRMMDV 155
>NP_054862
          Length = 290

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RTGRLMDI 8
           R GR+MD+
Sbjct: 262 RKGRMMDV 269
>NP_006161
          Length = 808

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GRLMDIF 9
           G+LMD+F
Sbjct: 282 GKLMDLF 288
>NP_001028886
          Length = 808

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GRLMDIF 9
           G+LMD+F
Sbjct: 282 GKLMDLF 288
>NP_001245239
          Length = 628

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GRLMDIF 9
           G+LMD+F
Sbjct: 282 GKLMDLF 288
>NP_001245237
          Length = 812

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GRLMDIF 9
           G+LMD+F
Sbjct: 286 GKLMDLF 292
>NP_001245238
          Length = 845

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GRLMDIF 9
           G+LMD+F
Sbjct: 319 GKLMDLF 325
>XP_005253748
          Length = 812

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GRLMDIF 9
           G+LMD+F
Sbjct: 286 GKLMDLF 292
>NP_055999
          Length = 1001

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RTGRLMDIF 9
          RTG+  DIF
Sbjct: 69 RTGKNTDIF 77
>NP_001137854
          Length = 1127

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RTGRLMDIF 9
           RTG+  DIF
Sbjct: 195 RTGKNTDIF 203
>NP_001160006
          Length = 575

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   GRLMDI 8
           GRLMDI
Sbjct: 275 GRLMDI 280
>NP_001160005
          Length = 704

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   GRLMDI 8
           GRLMDI
Sbjct: 404 GRLMDI 409
>XP_005248604
          Length = 666

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   GRLMDI 8
           GRLMDI
Sbjct: 366 GRLMDI 371
>XP_005248602
          Length = 700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   GRLMDI 8
           GRLMDI
Sbjct: 400 GRLMDI 405
>XP_006713136
          Length = 1022

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RTGRLMDIF 9
          RTG+  DIF
Sbjct: 90 RTGKNTDIF 98
>NP_001136021
          Length = 690

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   GRLMDI 8
           GRLMDI
Sbjct: 390 GRLMDI 395
>NP_077726
          Length = 724

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   GRLMDI 8
           GRLMDI
Sbjct: 424 GRLMDI 429
>NP_001191330
          Length = 497

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RTGRLMDI 8
           RTG+++DI
Sbjct: 142 RTGQVVDI 149
>NP_001158
          Length = 497

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RTGRLMDI 8
           RTG+++DI
Sbjct: 142 RTGQVVDI 149
>NP_006827
          Length = 2671

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    GRLMDIF 9
            GRLM+I+
Sbjct: 1199 GRLMEIY 1205
>XP_006724817
          Length = 497

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RTGRLMDI 8
           RTG+++DI
Sbjct: 142 RTGQVVDI 149
>XP_005269369
          Length = 681

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GRLMDIF 9
           GRL D+F
Sbjct: 527 GRLKDVF 533
>XP_005269368
          Length = 709

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GRLMDIF 9
           GRL D+F
Sbjct: 527 GRLKDVF 533
>XP_005269367
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GRLMDIF 9
           GRL D+F
Sbjct: 527 GRLKDVF 533
>NP_689912
          Length = 755

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GRLMDIF 9
           GRL D+F
Sbjct: 527 GRLKDVF 533
>NP_001124295
          Length = 1632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RTGRLMDIF 9
            R GR+ +IF
Sbjct: 1008 RIGRIKEIF 1016
>NP_001370
          Length = 1616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RTGRLMDIF 9
            R GR+ +IF
Sbjct: 992  RIGRIKEIF 1000
>NP_001166037
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TGRLMD 7
          TGRL+D
Sbjct: 84 TGRLLD 89
>NP_001166039
          Length = 264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TGRLMD 7
          TGRL+D
Sbjct: 84 TGRLLD 89
>NP_002459
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TGRLMD 7
          TGRL+D
Sbjct: 84 TGRLLD 89
>NP_001166040
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TGRLMD 7
          TGRL+D
Sbjct: 84 TGRLLD 89
>NP_001166038
          Length = 317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TGRLMD 7
          TGRL+D
Sbjct: 84 TGRLLD 89
>NP_036441
          Length = 1849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    TGRLMD 7
            TGRL+D
Sbjct: 1206 TGRLLD 1211
>NP_001277114
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   RTGRLMDIF 9
           ++GR  D+F
Sbjct: 645 KSGRFCDVF 653
>NP_068375
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   RTGRLMDIF 9
           ++GR  D+F
Sbjct: 655 KSGRFCDVF 663
>NP_573573
          Length = 1261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TGRLMD 7
           TGRL+D
Sbjct: 618 TGRLLD 623
>NP_061836
          Length = 2320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    TGRLMD 7
            TG+LMD
Sbjct: 1031 TGKLMD 1036
>NP_079175
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RTGRLMD 7
           R GRL+D
Sbjct: 328 RAGRLLD 334
>NP_001258918
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RTGRLMD 7
           R GRL+D
Sbjct: 253 RAGRLLD 259
>NP_057306
          Length = 622

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GRLMDI 8
           GRLMD+
Sbjct: 322 GRLMDL 327
>NP_387505
          Length = 2269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    TGRLMD 7
            TG+LMD
Sbjct: 1031 TGKLMD 1036
>XP_005261051
          Length = 2242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TGRLMD 7
           TG+LMD
Sbjct: 953 TGKLMD 958
>XP_006722744
          Length = 1495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTGRLMDIF 9
           R GR+ +IF
Sbjct: 871 RIGRIKEIF 879
>XP_006722420
          Length = 481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RTGRLMD 7
           R GRL+D
Sbjct: 150 RAGRLLD 156
>XP_006722419
          Length = 481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RTGRLMD 7
           R GRL+D
Sbjct: 150 RAGRLLD 156
>XP_005258207
          Length = 481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RTGRLMD 7
           R GRL+D
Sbjct: 150 RAGRLLD 156
>XP_005258206
          Length = 657

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RTGRLMD 7
           R GRL+D
Sbjct: 328 RAGRLLD 334
>XP_005277989
          Length = 1470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TGRLMD 7
           TGRL+D
Sbjct: 827 TGRLLD 832
>XP_006718541
          Length = 1839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    TGRLMD 7
            TGRL+D
Sbjct: 1196 TGRLLD 1201
>XP_006718540
          Length = 1842

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    TGRLMD 7
            TGRL+D
Sbjct: 1199 TGRLLD 1204
>XP_005277987
          Length = 1849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    TGRLMD 7
            TGRL+D
Sbjct: 1206 TGRLLD 1211
>XP_006714933
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GRLMDI 8
           GRLMD+
Sbjct: 196 GRLMDL 201
>XP_006713233
          Length = 213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TGRLMD 7
          TGRL+D
Sbjct: 84 TGRLLD 89
>XP_005265229
          Length = 258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TGRLMD 7
          TGRL+D
Sbjct: 84 TGRLLD 89
>XP_005245315
          Length = 1607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    TGRLMDIF 9
            +GR+ D+F
Sbjct: 1337 SGRISDVF 1344
>XP_006711447
          Length = 1634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    TGRLMDIF 9
            +GR+ D+F
Sbjct: 1364 SGRISDVF 1371
>XP_005245314
          Length = 1634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    TGRLMDIF 9
            +GR+ D+F
Sbjct: 1364 SGRISDVF 1371
>NP_002637
          Length = 1634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    TGRLMDIF 9
            +GR+ D+F
Sbjct: 1364 SGRISDVF 1371
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.159    0.476 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 496,962
Number of extensions: 835
Number of successful extensions: 66
Number of sequences better than 10000.0: 66
Number of HSP's gapped: 66
Number of HSP's successfully gapped: 66
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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