Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     390
NP_001075109                                                           20     390
NP_002518                                                              17    4312
NP_001096124                                                           17    4312
NP_078880                                                              17    4312
NP_001139547                                                           17    4312
NP_001139548                                                           17    4312
NP_001191355                                                           16    7355
NP_001244068                                                           16    7355
NP_055722                                                              16    7355
NP_001011703                                                           16    7355
NP_258257                                                              16    7355
NP_001268861                                                           16    7355
NP_060230                                                              16    7355
NP_001268862                                                           16    7355
NP_004061                                                              16    7355
NP_002305                                                              16    7355
XP_005252354                                                           16    7355
XP_005249604                                                           16    7355
NP_001036169                                                           16    7355
NP_036595                                                              15    9605
NP_001123488                                                           15    9605
NP_001032519                                                           15    9605
NP_775859                                                              15    9605
NP_055776                                                              15    9605
NP_001123489                                                           15    9605
NP_064506                                                              15    9605
NP_001170                                                              15    9605
NP_001258769                                                           15    9605
NP_001124181                                                           15    9605
NP_085150                                                              15    9605
NP_001363                                                              15    9605
XP_005253620                                                           15    9605
XP_006718010                                                           15    9605
XP_005263062                                                           15    9605
XP_005263061                                                           15    9605
XP_005263060                                                           15    9605
XP_005263059                                                           15    9605
XP_006714283                                                           15    9605
XP_005263056                                                           15    9605
XP_006714282                                                           15    9605
XP_006714281                                                           15    9605
XP_005263054                                                           15    9605
XP_005265716                                                           15    9605
XP_006714213                                                           15    9605
XP_006714212                                                           15    9605
XP_005265715                                                           15    9605
XP_005265714                                                           15    9605
XP_006714211                                                           15    9605
XP_005265713                                                           15    9605
XP_006713783                                                           15    9605
XP_005247745                                                           15    9605
XP_006713782                                                           15    9605
NP_004653                                                              15    9605
NP_006497                                                              15    9605
>NP_008835
          Length = 4128

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RTFLGELKT 9
           R FLGELKT
Sbjct: 198 RAFLGELKT 206
>NP_001075109
          Length = 4097

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RTFLGELKT 9
           R FLGELKT
Sbjct: 198 RAFLGELKT 206
>NP_002518
          Length = 257

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TFLGELKT 9
           T LGE+KT
Sbjct: 174 TVLGEIKT 181
>NP_001096124
          Length = 270

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TFLGELKT 9
           T LGE+KT
Sbjct: 187 TVLGEIKT 194
>NP_078880
          Length = 665

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RTFLGEL 7
           RTFLG+L
Sbjct: 360 RTFLGKL 366
>NP_001139547
          Length = 631

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RTFLGEL 7
           RTFLG+L
Sbjct: 360 RTFLGKL 366
>NP_001139548
          Length = 506

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RTFLGEL 7
           RTFLG+L
Sbjct: 201 RTFLGKL 207
>NP_001191355
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RTFLGELK 8
           RTFL E+K
Sbjct: 345 RTFLKEVK 352
>NP_001244068
          Length = 644

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RTFLGELKT 9
           RTFL  +KT
Sbjct: 261 RTFLSFIKT 269
>NP_055722
          Length = 1578

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LGELKT 9
           LGELKT
Sbjct: 390 LGELKT 395
>NP_001011703
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TFLGELKT 9
           TF+GEL +
Sbjct: 177 TFIGELNS 184
>NP_258257
          Length = 319

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TFLGELKT 9
           TF+GEL +
Sbjct: 177 TFIGELNS 184
>NP_001268861
          Length = 1143

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RTFLGELK 8
            R+FL ELK
Sbjct: 1060 RSFLDELK 1067
>NP_060230
          Length = 1143

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RTFLGELK 8
            R+FL ELK
Sbjct: 1060 RSFLDELK 1067
>NP_001268862
          Length = 1156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RTFLGELK 8
            R+FL ELK
Sbjct: 1060 RSFLDELK 1067
>NP_004061
          Length = 687

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RTFLGELKT 9
           RTFL  +KT
Sbjct: 304 RTFLSFIKT 312
>NP_002305
          Length = 647

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RTFLGELK 8
           RTFL E+K
Sbjct: 379 RTFLKEVK 386
>XP_005252354
          Length = 304

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TFLGELKT 9
           TF+GEL +
Sbjct: 162 TFIGELNS 169
>XP_005249604
          Length = 1096

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RTFLGELK 8
            R+FL ELK
Sbjct: 1013 RSFLDELK 1020
>NP_001036169
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RTFLGELKT 9
           RTFL  +KT
Sbjct: 304 RTFLSFIKT 312
>NP_036595
          Length = 856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  RTFLGELK 8
          R F+GE+K
Sbjct: 49 RKFVGEVK 56
>NP_001123488
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>NP_001032519
          Length = 620

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RTFLGELKT 9
           +TFLG+ K+
Sbjct: 120 KTFLGQNKS 128
>NP_775859
          Length = 1326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FLGELK 8
           FLGE+K
Sbjct: 330 FLGEMK 335
>NP_055776
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RTFLGELKT 9
           +TFLG+ K+
Sbjct: 120 KTFLGQNKS 128
>NP_001123489
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>NP_064506
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RTFLGEL 7
           R+FLG+L
Sbjct: 807 RSFLGQL 813
>NP_001170
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>NP_001258769
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RTFLGELK 8
          RTF G+LK
Sbjct: 76 RTFDGKLK 83
>NP_001124181
          Length = 506

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RTFLGELKT 9
           +TFLG+ K+
Sbjct: 180 KTFLGQNKS 188
>NP_085150
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RTFLGELKT 9
           RTF+ EL++
Sbjct: 120 RTFIKELRS 128
>NP_001363
          Length = 4486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    RTFLGE 6
            RT+LGE
Sbjct: 4128 RTYLGE 4133
>XP_005253620
          Length = 816

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  RTFLGELK 8
          R F+GE+K
Sbjct: 49 RKFVGEVK 56
>XP_006718010
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RTFLGELK 8
          RTF G+LK
Sbjct: 76 RTFDGKLK 83
>XP_005263062
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>XP_005263061
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>XP_005263060
          Length = 357

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>XP_005263059
          Length = 363

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>XP_006714283
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>XP_005263056
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>XP_006714282
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>XP_006714281
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>XP_005263054
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FLGELKT 9
           FLG LKT
Sbjct: 258 FLGGLKT 264
>XP_005265716
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RTFLGELKT 9
           +TFLG+ K+
Sbjct: 138 KTFLGQNKS 146
>XP_006714213
          Length = 544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RTFLGELKT 9
           +TFLG+ K+
Sbjct: 138 KTFLGQNKS 146
>XP_006714212
          Length = 545

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RTFLGELKT 9
           +TFLG+ K+
Sbjct: 138 KTFLGQNKS 146
>XP_005265715
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  RTFLGELKT 9
          +TFLG+ K+
Sbjct: 56 KTFLGQNKS 64
>XP_005265714
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  RTFLGELKT 9
          +TFLG+ K+
Sbjct: 67 KTFLGQNKS 75
>XP_006714211
          Length = 615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RTFLGELKT 9
           +TFLG+ K+
Sbjct: 138 KTFLGQNKS 146
>XP_005265713
          Length = 638

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RTFLGELKT 9
           +TFLG+ K+
Sbjct: 138 KTFLGQNKS 146
>XP_006713783
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  FLGELK 8
          FLGE+K
Sbjct: 47 FLGEIK 52
>XP_005247745
          Length = 850

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FLGELK 8
           FLGE+K
Sbjct: 268 FLGEIK 273
>XP_006713782
          Length = 854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FLGELK 8
           FLGE+K
Sbjct: 268 FLGEIK 273
>NP_004653
          Length = 798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RTFLGE 6
           RT+LGE
Sbjct: 440 RTYLGE 445
>NP_006497
          Length = 849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FLGELK 8
           FLGE+K
Sbjct: 268 FLGEIK 273
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.142    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 460,958
Number of extensions: 632
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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