Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              20     299
NP_003794                                                              18    1135
NP_062830                                                              18    1135
NP_078858                                                              17    2528
NP_001278232                                                           17    2528
NP_001278214                                                           17    2528
NP_001271169                                                           17    2528
NP_001271170                                                           17    2528
NP_061872                                                              17    2528
XP_005269963                                                           17    2528
XP_005269962                                                           17    2528
XP_005256013                                                           17    4312
NP_001167535                                                           16    7355
NP_001167534                                                           16    7355
NP_001167536                                                           16    7355
NP_000312                                                              16    7355
NP_056934                                                              16    7355
NP_075598                                                              16    7355
NP_001167538                                                           16    7355
NP_001035915                                                           16    7355
NP_689564                                                              16    7355
NP_001257578                                                           16    7355
NP_001257577                                                           16    7355
XP_005258838                                                           16    7355
XP_006721514                                                           16    7355
XP_006721513                                                           16    7355
XP_005256514                                                           16    7355
XP_006716372                                                           16    7355
XP_006716371                                                           16    7355
XP_006716370                                                           16    7355
XP_006716369                                                           16    7355
XP_006716368                                                           16    7355
XP_006716367                                                           16    7355
XP_006716366                                                           16    7355
NP_001157576                                                           15    9605
NP_001157577                                                           15    9605
NP_061868                                                              15    9605
NP_061325                                                              15    9605
NP_940998                                                              15    9605
NP_001155313                                                           15    9605
NP_775922                                                              15    9605
XP_006724481                                                           15    9605
XP_005261980                                                           15    9605
XP_006723893                                                           15    9605
XP_005260515                                                           15    9605
XP_006723892                                                           15    9605
XP_006723891                                                           15    9605
XP_006723890                                                           15    9605
>NP_004351
          Length = 882

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  RTAYFSLNT 9
          RTAYFSL+T
Sbjct: 65 RTAYFSLDT 73
>NP_003794
          Length = 1685

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    RTAYFSLN 8
            RTAYF++N
Sbjct: 1627 RTAYFTIN 1634
>NP_062830
          Length = 1589

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    RTAYFSLN 8
            RTAYF++N
Sbjct: 1531 RTAYFTIN 1538
>NP_078858
          Length = 4981

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    TAYFSLNT 9
            T+YFSL+T
Sbjct: 3555 TSYFSLST 3562
>NP_001278232
          Length = 4983

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    TAYFSLNT 9
            T+YFSL+T
Sbjct: 3557 TSYFSLST 3564
>NP_001278214
          Length = 4982

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    TAYFSLNT 9
            T+YFSL+T
Sbjct: 3557 TSYFSLST 3564
>NP_001271169
          Length = 1047

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RTAYFSLNT 9
           RT+Y S+NT
Sbjct: 160 RTSYSSINT 168
>NP_001271170
          Length = 567

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RTAYFSLNT 9
           RT+Y S+NT
Sbjct: 160 RTSYSSINT 168
>NP_061872
          Length = 834

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RTAYFSLNT 9
           RT+Y S+NT
Sbjct: 160 RTSYSSINT 168
>XP_005269963
          Length = 837

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RTAYFSLNT 9
           RT+Y S+NT
Sbjct: 160 RTSYSSINT 168
>XP_005269962
          Length = 850

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RTAYFSLNT 9
           RT+Y S+NT
Sbjct: 160 RTSYSSINT 168
>XP_005256013
          Length = 127

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RTAYFSLNT 9
          RTA+FS +T
Sbjct: 86 RTAFFSWDT 94
>NP_001167535
          Length = 812

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 103 TTYFSVN 109
>NP_001167534
          Length = 820

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 111 TTYFSVN 117
>NP_001167536
          Length = 820

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 111 TTYFSVN 117
>NP_000312
          Length = 928

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RTAYFSLNT 9
           R AY  LNT
Sbjct: 656 RLAYLRLNT 664
>NP_056934
          Length = 820

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 111 TTYFSVN 117
>NP_075598
          Length = 822

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 111 TTYFSVN 117
>NP_001167538
          Length = 853

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 144 TTYFSVN 150
>NP_001035915
          Length = 596

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TAYFSLN 8
          T YF+LN
Sbjct: 25 TVYFALN 31
>NP_689564
          Length = 612

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TAYFSLN 8
          T YF+LN
Sbjct: 25 TVYFALN 31
>NP_001257578
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TAYFSLN 8
          T YF+LN
Sbjct: 25 TVYFALN 31
>NP_001257577
          Length = 569

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TAYFSLN 8
          T YF+LN
Sbjct: 25 TVYFALN 31
>XP_005258838
          Length = 563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TAYFSLNT 9
           T YFS++T
Sbjct: 548 TEYFSMHT 555
>XP_006721514
          Length = 596

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TAYFSLN 8
          T YF+LN
Sbjct: 25 TVYFALN 31
>XP_006721513
          Length = 598

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TAYFSLN 8
          T YF+LN
Sbjct: 25 TVYFALN 31
>XP_005256514
          Length = 633

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TAYFSLN 8
          T YF+LN
Sbjct: 25 TVYFALN 31
>XP_006716372
          Length = 824

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 103 TTYFSVN 109
>XP_006716371
          Length = 830

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 111 TTYFSVN 117
>XP_006716370
          Length = 830

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 111 TTYFSVN 117
>XP_006716369
          Length = 830

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 111 TTYFSVN 117
>XP_006716368
          Length = 832

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 111 TTYFSVN 117
>XP_006716367
          Length = 832

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 111 TTYFSVN 117
>XP_006716366
          Length = 832

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TAYFSLN 8
           T YFS+N
Sbjct: 111 TTYFSVN 117
>NP_001157576
          Length = 1165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTAYFSLNT 9
           +T YFSL +
Sbjct: 136 QTTYFSLES 144
>NP_001157577
          Length = 1165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTAYFSLNT 9
           +T YFSL +
Sbjct: 116 QTTYFSLES 124
>NP_061868
          Length = 1211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTAYFSLNT 9
           +T YFSL +
Sbjct: 136 QTTYFSLES 144
>NP_061325
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLNT 9
           YF LNT
Sbjct: 781 YFDLNT 786
>NP_940998
          Length = 867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLNT 9
           YF LNT
Sbjct: 781 YFDLNT 786
>NP_001155313
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLNT 9
           YF LNT
Sbjct: 781 YFDLNT 786
>NP_775922
          Length = 6996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    AYFSLNT 9
            A+FS NT
Sbjct: 4450 AFFSFNT 4456
>XP_006724481
          Length = 1164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTAYFSLNT 9
           +T YFSL +
Sbjct: 136 QTTYFSLES 144
>XP_005261980
          Length = 1191

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTAYFSLNT 9
           +T YFSL +
Sbjct: 116 QTTYFSLES 124
>XP_006723893
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLNT 9
           YF LNT
Sbjct: 781 YFDLNT 786
>XP_005260515
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLNT 9
           YF LNT
Sbjct: 781 YFDLNT 786
>XP_006723892
          Length = 871

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLNT 9
           YF LNT
Sbjct: 782 YFDLNT 787
>XP_006723891
          Length = 871

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLNT 9
           YF LNT
Sbjct: 782 YFDLNT 787
>XP_006723890
          Length = 871

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLNT 9
           YF LNT
Sbjct: 782 YFDLNT 787
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.126    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 421,803
Number of extensions: 528
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package