Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_079123                                                              17    2528
NP_001153836                                                           17    2528
XP_005255657                                                           17    2528
XP_006721158                                                           17    2528
XP_006721157                                                           17    2528
XP_005255654                                                           17    2528
NP_060371                                                              17    3301
XP_006719528                                                           17    3301
XP_005253963                                                           17    3301
XP_005253962                                                           17    3301
NP_001137291                                                           17    3301
NP_066997                                                              16    5631
NP_443090                                                              16    5631
XP_006724300                                                           16    5631
NP_055514                                                              16    7355
NP_001177738                                                           16    7355
NP_001014448                                                           16    7355
NP_001014447                                                           16    7355
NP_003643                                                              16    7355
XP_005255769                                                           16    7355
XP_005255768                                                           16    7355
XP_005255767                                                           16    7355
XP_005264262                                                           16    7355
NP_068750                                                              16    7355
NP_001155155                                                           15    9605
NP_653273                                                              15    9605
NP_001714                                                              15    9605
NP_001010870                                                           15    9605
NP_001161831                                                           15    9605
NP_001001671                                                           15    9605
XP_006726824                                                           15    9605
XP_006722329                                                           15    9605
XP_005249383                                                           15    9605
XP_006710096                                                           15    9605
>NP_079123
          Length = 723

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TALRCVFV 9
           T LRCVF+
Sbjct: 443 TILRCVFM 450
>NP_001153836
          Length = 613

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TALRCVFV 9
           T LRCVF+
Sbjct: 333 TILRCVFM 340
>XP_005255657
          Length = 381

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TALRCVFV 9
           T LRCVF+
Sbjct: 101 TILRCVFM 108
>XP_006721158
          Length = 625

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TALRCVFV 9
           T LRCVF+
Sbjct: 345 TILRCVFM 352
>XP_006721157
          Length = 671

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TALRCVFV 9
           T LRCVF+
Sbjct: 443 TILRCVFM 450
>XP_005255654
          Length = 758

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TALRCVFV 9
           T LRCVF+
Sbjct: 443 TILRCVFM 450
>NP_060371
          Length = 816

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALRCVFV 9
           ALRC+F+
Sbjct: 203 ALRCIFL 209
>XP_006719528
          Length = 641

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALRCVFV 9
           ALRC+F+
Sbjct: 275 ALRCIFL 281
>XP_005253963
          Length = 748

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALRCVFV 9
           ALRC+F+
Sbjct: 135 ALRCIFL 141
>XP_005253962
          Length = 870

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALRCVFV 9
           ALRC+F+
Sbjct: 275 ALRCIFL 281
>NP_001137291
          Length = 888

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALRCVFV 9
           ALRC+F+
Sbjct: 275 ALRCIFL 281
>NP_066997
          Length = 923

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RTALRC 6
           RTA+RC
Sbjct: 382 RTAIRC 387
>NP_443090
          Length = 401

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TALRCVFV 9
           TAL C+FV
Sbjct: 281 TALCCLFV 288
>XP_006724300
          Length = 242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TALRCVF 8
          TAL C+F
Sbjct: 89 TALACIF 95
>NP_055514
          Length = 1832

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    RTALRC 6
            RTA+RC
Sbjct: 1361 RTAVRC 1366
>NP_001177738
          Length = 672

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTALRCVFV 9
           RTA+ C+ V
Sbjct: 340 RTAIECLVV 348
>NP_001014448
          Length = 515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RTALRCVFV 9
          RTA RC  V
Sbjct: 62 RTASRCAHV 70
>NP_001014447
          Length = 652

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RTALRCVFV 9
           RTA RC  V
Sbjct: 199 RTASRCAHV 207
>NP_003643
          Length = 641

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RTALRCVFV 9
           RTA RC  V
Sbjct: 188 RTASRCAHV 196
>XP_005255769
          Length = 1808

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    RTALRC 6
            RTA+RC
Sbjct: 1361 RTAVRC 1366
>XP_005255768
          Length = 1832

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    RTALRC 6
            RTA+RC
Sbjct: 1361 RTAVRC 1366
>XP_005255767
          Length = 1832

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    RTALRC 6
            RTA+RC
Sbjct: 1361 RTAVRC 1366
>XP_005264262
          Length = 543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  RTALRC 6
          RT+LRC
Sbjct: 37 RTSLRC 42
>NP_068750
          Length = 703

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RTALRCVFV 9
           RTA+ C+ V
Sbjct: 371 RTAIECLVV 379
>NP_001155155
          Length = 1060

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  RTALRCV 7
          RTA++C+
Sbjct: 40 RTAMKCL 46
>NP_653273
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   RTALRCV 7
           RTA++C+
Sbjct: 119 RTAMKCL 125
>NP_001714
          Length = 2649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    TALRCV 7
            TALRC+
Sbjct: 2475 TALRCL 2480
>NP_001010870
          Length = 2096

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    ALRCVFV 9
            A+RC FV
Sbjct: 1446 AIRCEFV 1452
>NP_001161831
          Length = 2066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    ALRCVFV 9
            A+RC FV
Sbjct: 1446 AIRCEFV 1452
>NP_001001671
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RTALRCVFV 9
          R ALR V+V
Sbjct: 58 RRALRAVYV 66
>XP_006726824
          Length = 216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   ALRCVF 8
           ALRC F
Sbjct: 96  ALRCAF 101
>XP_006722329
          Length = 216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   ALRCVF 8
           ALRC F
Sbjct: 96  ALRCAF 101
>XP_005249383
          Length = 3186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    TALRCV 7
            TALRC+
Sbjct: 3012 TALRCL 3017
>XP_006710096
          Length = 216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   ALRCVF 8
           ALRC F
Sbjct: 96  ALRCAF 101
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.355    0.149    0.503 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 490,209
Number of extensions: 512
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.1 bits)
S2: 28 (15.4 bits)

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