Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_853559                                                              16    5631
NP_001257348                                                           16    5631
XP_006715908                                                           16    5631
XP_006715907                                                           16    5631
XP_005250182                                                           16    5631
NP_001124320                                                           16    7355
NP_004901                                                              16    7355
NP_490595                                                              16    7355
XP_005263889                                                           16    7355
XP_006712890                                                           16    7355
XP_005263888                                                           16    7355
XP_005263887                                                           16    7355
NP_001232                                                              16    7355
NP_689728                                                              15    9605
NP_001243028                                                           15    9605
XP_006718871                                                           15    9605
NP_835228                                                              15    9605
>NP_853559
          Length = 317

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SVSLKLRI 9
           SVS+K+RI
Sbjct: 154 SVSIKIRI 161
>NP_001257348
          Length = 317

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SVSLKLRI 9
           SVS+K+RI
Sbjct: 154 SVSIKIRI 161
>XP_006715908
          Length = 237

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SVSLKLRI 9
          SVS+K+RI
Sbjct: 74 SVSIKIRI 81
>XP_006715907
          Length = 237

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SVSLKLRI 9
          SVS+K+RI
Sbjct: 74 SVSIKIRI 81
>XP_005250182
          Length = 317

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SVSLKLRI 9
           SVS+K+RI
Sbjct: 154 SVSIKIRI 161
>NP_001124320
          Length = 1243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RSVSLKL 7
            RS+SLKL
Sbjct: 1233 RSISLKL 1239
>NP_004901
          Length = 1244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RSVSLKL 7
            RS+SLKL
Sbjct: 1234 RSISLKL 1240
>NP_490595
          Length = 730

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RSVSLKLRI 9
           +S SLKLRI
Sbjct: 501 KSGSLKLRI 509
>XP_005263889
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RSVSLKLRI 9
           +S SLKLRI
Sbjct: 326 KSGSLKLRI 334
>XP_006712890
          Length = 586

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RSVSLKLRI 9
           +S SLKLRI
Sbjct: 357 KSGSLKLRI 365
>XP_005263888
          Length = 586

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RSVSLKLRI 9
           +S SLKLRI
Sbjct: 357 KSGSLKLRI 365
>XP_005263887
          Length = 709

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RSVSLKLRI 9
           +S SLKLRI
Sbjct: 480 KSGSLKLRI 488
>NP_001232
          Length = 663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RSVSLKLRI 9
           +S SLKLRI
Sbjct: 501 KSGSLKLRI 509
>NP_689728
          Length = 745

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  RSVSLKLR 8
          RS+S+KL+
Sbjct: 72 RSISIKLQ 79
>NP_001243028
          Length = 229

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  RSVSLKLR 8
          RS+++KLR
Sbjct: 6  RSITVKLR 13
>XP_006718871
          Length = 323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RSVSLKLR 8
           +SVS+K+R
Sbjct: 308 KSVSMKIR 315
>NP_835228
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RSVSLKLR 8
           +SVS+K+R
Sbjct: 373 KSVSMKIR 380
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.141    0.326 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 296,924
Number of extensions: 294
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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