Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1482
NP_671714                                                              18    1482
XP_006715890                                                           18    1482
XP_005250160                                                           18    1482
XP_005250159                                                           18    1482
NP_001166245                                                           18    1935
XP_005258618                                                           18    1935
NP_997208                                                              18    1935
NP_570853                                                              16    7355
NP_570854                                                              16    7355
NP_000453                                                              16    7355
NP_055928                                                              16    7355
NP_001245344                                                           16    7355
NP_065860                                                              16    7355
NP_001245347                                                           16    7355
NP_001245346                                                           16    7355
NP_001245345                                                           16    7355
XP_005256618                                                           16    7355
XP_005256617                                                           16    7355
XP_005268328                                                           16    7355
XP_006720739                                                           16    7355
XP_005268327                                                           16    7355
XP_005268326                                                           16    7355
XP_005268324                                                           16    7355
XP_005268325                                                           16    7355
XP_006720738                                                           16    7355
XP_006720737                                                           16    7355
XP_006720736                                                           16    7355
XP_006718956                                                           16    7355
NP_058640                                                              15    9605
NP_008864                                                              15    9605
NP_000334                                                              15    9605
NP_570855                                                              15    9605
NP_570856                                                              15    9605
NP_065683                                                              15    9605
NP_001243243                                                           15    9605
NP_065803                                                              15    9605
XP_005250451                                                           15    9605
XP_005250450                                                           15    9605
XP_006713672                                                           15    9605
NP_060523                                                              15    9605
>NP_005742
          Length = 3907

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RSVLLAAF 8
            RSVLLAAF
Sbjct: 3038 RSVLLAAF 3045
>NP_671714
          Length = 3899

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RSVLLAAF 8
            RSVLLAAF
Sbjct: 3030 RSVLLAAF 3037
>XP_006715890
          Length = 3860

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RSVLLAAF 8
            RSVLLAAF
Sbjct: 2991 RSVLLAAF 2998
>XP_005250160
          Length = 3939

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RSVLLAAF 8
            RSVLLAAF
Sbjct: 3070 RSVLLAAF 3077
>XP_005250159
          Length = 3952

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RSVLLAAF 8
            RSVLLAAF
Sbjct: 3083 RSVLLAAF 3090
>NP_001166245
          Length = 716

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SVLLAAFW 9
           S LLAAFW
Sbjct: 187 SGLLAAFW 194
>XP_005258618
          Length = 682

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SVLLAAFW 9
           S LLAAFW
Sbjct: 187 SGLLAAFW 194
>NP_997208
          Length = 716

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SVLLAAFW 9
           S LLAAFW
Sbjct: 187 SGLLAAFW 194
>NP_570853
          Length = 852

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 761 SVLIREFW 768
>NP_570854
          Length = 872

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 781 SVLIREFW 788
>NP_000453
          Length = 875

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 784 SVLIREFW 791
>NP_055928
          Length = 2961

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL + FW
Sbjct: 958 SVLFSLFW 965
>NP_001245344
          Length = 1168

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RSVLLAAFW 9
           RS++  AFW
Sbjct: 309 RSIINWAFW 317
>NP_065860
          Length = 1191

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RSVLLAAFW 9
           RS++  AFW
Sbjct: 332 RSIINWAFW 340
>NP_001245347
          Length = 1155

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RSVLLAAFW 9
           RS++  AFW
Sbjct: 296 RSIINWAFW 304
>NP_001245346
          Length = 1155

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RSVLLAAFW 9
           RS++  AFW
Sbjct: 296 RSIINWAFW 304
>NP_001245345
          Length = 1165

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RSVLLAAFW 9
           RS++  AFW
Sbjct: 306 RSIINWAFW 314
>XP_005256618
          Length = 2962

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL + FW
Sbjct: 959 SVLFSLFW 966
>XP_005256617
          Length = 2966

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL + FW
Sbjct: 959 SVLFSLFW 966
>XP_005268328
          Length = 852

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 761 SVLIREFW 768
>XP_006720739
          Length = 852

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 761 SVLIREFW 768
>XP_005268327
          Length = 852

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 761 SVLIREFW 768
>XP_005268326
          Length = 852

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 761 SVLIREFW 768
>XP_005268324
          Length = 852

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 761 SVLIREFW 768
>XP_005268325
          Length = 852

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 761 SVLIREFW 768
>XP_006720738
          Length = 852

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 761 SVLIREFW 768
>XP_006720737
          Length = 872

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 781 SVLIREFW 788
>XP_006720736
          Length = 872

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVL+  FW
Sbjct: 781 SVLIREFW 788
>XP_006718956
          Length = 1191

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RSVLLAAFW 9
           RS++  AFW
Sbjct: 332 RSIINWAFW 340
>NP_058640
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RSVLLAAFW 9
          RSV L+AF+
Sbjct: 76 RSVYLSAFF 84
>NP_008864
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   LLAAFW 9
           LLA FW
Sbjct: 457 LLAIFW 462
>NP_000334
          Length = 664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   LLAAFW 9
           LLA FW
Sbjct: 472 LLAIFW 477
>NP_570855
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VLLAAFW 9
           +LLAA W
Sbjct: 418 MLLAALW 424
>NP_570856
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VLLAAFW 9
           +LLAA W
Sbjct: 418 MLLAALW 424
>NP_065683
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VLLAAFW 9
           +LLAA W
Sbjct: 418 MLLAALW 424
>NP_001243243
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   LLAAFW 9
           LLA FW
Sbjct: 345 LLAIFW 350
>NP_065803
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 5/7 (71%)

Query: 3   VLLAAFW 9
           +LL  FW
Sbjct: 533 ILLVVFW 539
>XP_005250451
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VLLAAFW 9
           +LLAA W
Sbjct: 418 MLLAALW 424
>XP_005250450
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VLLAAFW 9
           +LLAA W
Sbjct: 418 MLLAALW 424
>XP_006713672
          Length = 452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           SVLL A W
Sbjct: 445 SVLLLASW 452
>NP_060523
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   SVLLAAFW 9
           S+LL A W
Sbjct: 296 SILLVATW 303
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.135    0.506 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 306,917
Number of extensions: 450
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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