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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              18    1482
NP_001231892                                                           16    5631
NP_001104488                                                           16    5631
NP_660093                                                              16    5631
NP_892009                                                              16    5631
XP_006718432                                                           16    5631
XP_006718430                                                           16    5631
XP_006718429                                                           16    5631
XP_006718428                                                           16    5631
XP_006718427                                                           16    5631
NP_055994                                                              16    7355
NP_001138586                                                           16    7355
NP_008890                                                              16    7355
XP_006723416                                                           16    7355
XP_006723415                                                           16    7355
XP_006723414                                                           16    7355
NP_821174                                                              15    9605
NP_060340                                                              15    9605
NP_067027                                                              15    9605

>NP_004251
          Length = 1208

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RSTLLRKQA 9
           RS LLRKQA
Sbjct: 370 RSRLLRKQA 378



>NP_001231892
          Length = 2488

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RSTLLRKQ 8
            RSTL RKQ
Sbjct: 1493 RSTLPRKQ 1500



>NP_001104488
          Length = 2365

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RSTLLRKQ 8
            RSTL RKQ
Sbjct: 1406 RSTLPRKQ 1413



>NP_660093
          Length = 2429

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RSTLLRKQ 8
            RSTL RKQ
Sbjct: 1470 RSTLPRKQ 1477



>NP_892009
          Length = 2432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RSTLLRKQ 8
            RSTL RKQ
Sbjct: 1470 RSTLPRKQ 1477



>XP_006718432
          Length = 1464

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RSTLLRKQ 8
           RSTL RKQ
Sbjct: 469 RSTLPRKQ 476



>XP_006718430
          Length = 1551

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RSTLLRKQ 8
           RSTL RKQ
Sbjct: 556 RSTLPRKQ 563



>XP_006718429
          Length = 2416

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RSTLLRKQ 8
            RSTL RKQ
Sbjct: 1421 RSTLPRKQ 1428



>XP_006718428
          Length = 2455

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RSTLLRKQ 8
            RSTL RKQ
Sbjct: 1493 RSTLPRKQ 1500



>XP_006718427
          Length = 2465

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RSTLLRKQ 8
            RSTL RKQ
Sbjct: 1470 RSTLPRKQ 1477



>NP_055994
          Length = 1297

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   STLLRKQ 8
           +TLLRKQ
Sbjct: 218 ATLLRKQ 224



>NP_001138586
          Length = 1236

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   STLLRKQ 8
           +TLLRKQ
Sbjct: 157 ATLLRKQ 163



>NP_008890
          Length = 662

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RSTLLRKQ 8
           RSTL+R Q
Sbjct: 398 RSTLIRHQ 405



>XP_006723416
          Length = 513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RSTLLRKQ 8
           RSTL+R Q
Sbjct: 249 RSTLIRHQ 256



>XP_006723415
          Length = 664

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RSTLLRKQ 8
           RSTL+R Q
Sbjct: 400 RSTLIRHQ 407



>XP_006723414
          Length = 664

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RSTLLRKQ 8
           RSTL+R Q
Sbjct: 400 RSTLIRHQ 407



>NP_821174
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   STLLRKQA 9
           STL RK+A
Sbjct: 949 STLARKEA 956



>NP_060340
          Length = 1024

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   STLLRKQA 9
           STL RK+A
Sbjct: 950 STLARKEA 957



>NP_067027
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  TLLRKQA 9
          +LLRKQA
Sbjct: 57 SLLRKQA 63



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.124    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 402,571
Number of extensions: 375
Number of successful extensions: 19
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 19
Number of HSP's successfully gapped: 19
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)