Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              19     665
NP_005059                                                              17    4312
XP_005261949                                                           17    4312
XP_005267157                                                           17    4312
XP_005275966                                                           17    4312
NP_001005280                                                           16    5631
NP_001005226                                                           16    5631
NP_001182000                                                           16    5631
NP_001005192                                                           16    5631
NP_036396                                                              16    5631
NP_001182001                                                           16    5631
NP_039229                                                              16    5631
XP_005261571                                                           16    5631
XP_005261570                                                           16    5631
XP_005261569                                                           16    5631
XP_006724290                                                           16    5631
XP_005261568                                                           16    5631
XP_005261567                                                           16    5631
XP_005261566                                                           16    5631
NP_149046                                                              16    5631
NP_853631                                                              16    7355
NP_001278238                                                           15    9605
NP_612192                                                              15    9605
NP_002561                                                              15    9605
NP_115486                                                              15    9605
NP_005975                                                              15    9605
NP_001243463                                                           15    9605
NP_060897                                                              15    9605
NP_060043                                                              15    9605
XP_005259643                                                           15    9605
XP_005259642                                                           15    9605
XP_005259640                                                           15    9605
XP_006722679                                                           15    9605
NP_001078844                                                           15    9605
NP_001128712                                                           15    9605
>NP_073713
          Length = 985

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RSSLMYGTA 9
          RSSL YGTA
Sbjct: 38 RSSLTYGTA 46
>NP_005059
          Length = 766

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RSSLMYGTA 9
           RSSL YG A
Sbjct: 442 RSSLCYGFA 450
>XP_005261949
          Length = 210

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RSSLMYGT 8
           RSS +YGT
Sbjct: 187 RSSTIYGT 194
>XP_005267157
          Length = 766

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RSSLMYGTA 9
           RSSL YG A
Sbjct: 442 RSSLCYGFA 450
>XP_005275966
          Length = 210

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RSSLMYGT 8
           RSS +YGT
Sbjct: 187 RSSTIYGT 194
>NP_001005280
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLMYGTA 9
           SL YGTA
Sbjct: 249 SLFYGTA 255
>NP_001005226
          Length = 313

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLMYGTA 9
           SL YGTA
Sbjct: 249 SLFYGTA 255
>NP_001182000
          Length = 407

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSLMYGT 8
           SS MYGT
Sbjct: 151 SSCMYGT 157
>NP_001005192
          Length = 311

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLMYGTA 9
           SL YGTA
Sbjct: 249 SLFYGTA 255
>NP_036396
          Length = 407

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSLMYGT 8
           SS MYGT
Sbjct: 151 SSCMYGT 157
>NP_001182001
          Length = 341

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  SSLMYGT 8
          SS MYGT
Sbjct: 85 SSCMYGT 91
>NP_039229
          Length = 312

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLMYGTA 9
           SL YGTA
Sbjct: 248 SLFYGTA 254
>XP_005261571
          Length = 348

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  SSLMYGT 8
          SS MYGT
Sbjct: 85 SSCMYGT 91
>XP_005261570
          Length = 348

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  SSLMYGT 8
          SS MYGT
Sbjct: 85 SSCMYGT 91
>XP_005261569
          Length = 365

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSLMYGT 8
           SS MYGT
Sbjct: 184 SSCMYGT 190
>XP_006724290
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSLMYGT 8
           SS MYGT
Sbjct: 151 SSCMYGT 157
>XP_005261568
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSLMYGT 8
           SS MYGT
Sbjct: 151 SSCMYGT 157
>XP_005261567
          Length = 440

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSLMYGT 8
           SS MYGT
Sbjct: 184 SSCMYGT 190
>XP_005261566
          Length = 447

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSLMYGT 8
           SS MYGT
Sbjct: 184 SSCMYGT 190
>NP_149046
          Length = 357

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SLMYGTA 9
           SL YGTA
Sbjct: 249 SLFYGTA 255
>NP_853631
          Length = 160

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SSLMYGTA 9
          SSL+Y TA
Sbjct: 27 SSLVYSTA 34
>NP_001278238
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SSLMYGTA 9
           S ++YGTA
Sbjct: 552 SLILYGTA 559
>NP_612192
          Length = 956

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SSLMYGTA 9
           S ++YGTA
Sbjct: 626 SLILYGTA 633
>NP_002561
          Length = 969

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SSLMYGTA 9
           S ++YGTA
Sbjct: 626 SLILYGTA 633
>NP_115486
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LMYGTA 9
           L+YGTA
Sbjct: 131 LLYGTA 136
>NP_005975
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SSLMYGT 8
          SSL+YG+
Sbjct: 88 SSLLYGS 94
>NP_001243463
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SSLMYGT 8
           SSL+YG+
Sbjct: 95  SSLLYGS 101
>NP_060897
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RSSLMYGT 8
           RSS+MY T
Sbjct: 96  RSSIMYLT 103
>NP_060043
          Length = 755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LMYGTA 9
           L+YGTA
Sbjct: 574 LLYGTA 579
>XP_005259643
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LMYGTA 9
           L+YGTA
Sbjct: 386 LLYGTA 391
>XP_005259642
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LMYGTA 9
           L+YGTA
Sbjct: 574 LLYGTA 579
>XP_005259640
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LMYGTA 9
           L+YGTA
Sbjct: 565 LLYGTA 570
>XP_006722679
          Length = 2128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    LMYGTA 9
            L+YGTA
Sbjct: 1624 LLYGTA 1629
>NP_001078844
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSLMYGT 8
           SSL YGT
Sbjct: 146 SSLEYGT 152
>NP_001128712
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SSLMYGT 8
           SSL YGT
Sbjct: 189 SSLEYGT 195
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.121    0.322 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 390,747
Number of extensions: 421
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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