Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     869
XP_006716142                                                           19     869
XP_005250088                                                           19     869
XP_006716141                                                           19     869
XP_005250085                                                           19     869
XP_006716140                                                           19     869
XP_005250084                                                           19     869
XP_005250083                                                           19     869
XP_005250082                                                           19     869
XP_006726491                                                           18    1135
XP_006715877                                                           18    1135
XP_006710244                                                           18    1135
NP_001243000                                                           18    1482
NP_066005                                                              18    1482
XP_005257603                                                           18    1482
XP_006722058                                                           18    1482
XP_005257602                                                           18    1482
NP_542942                                                              16    5631
XP_006725464                                                           16    5631
XP_006725463                                                           16    5631
XP_005277590                                                           16    5631
XP_005261395                                                           16    5631
NP_000294                                                              15    9605
NP_079133                                                              15    9605
NP_000238                                                              15    9605
NP_001334                                                              15    9605
NP_006805                                                              15    9605
NP_848693                                                              15    9605
NP_001231892                                                           15    9605
NP_001136156                                                           15    9605
NP_660093                                                              15    9605
NP_001034739                                                           15    9605
NP_001034738                                                           15    9605
NP_004843                                                              15    9605
NP_060480                                                              15    9605
NP_892009                                                              15    9605
NP_004489                                                              15    9605
XP_006723727                                                           15    9605
XP_005260931                                                           15    9605
XP_005260930                                                           15    9605
XP_005255429                                                           15    9605
XP_005267824                                                           15    9605
XP_005266651                                                           15    9605
XP_006719957                                                           15    9605
XP_005266650                                                           15    9605
XP_005266578                                                           15    9605
XP_006718428                                                           15    9605
XP_006718427                                                           15    9605
NP_059133                                                              15    9605
NP_001073957                                                           15    9605
>NP_733751
          Length = 4911

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 909 RSKLKSGI 916
>XP_006716142
          Length = 4927

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_005250088
          Length = 4928

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_006716141
          Length = 4959

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_005250085
          Length = 4979

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_006716140
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_005250084
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_005250083
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 909 RSKLKSGI 916
>XP_005250082
          Length = 4983

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_006726491
          Length = 230

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSG+
Sbjct: 167 RSKLKSGV 174
>XP_006715877
          Length = 230

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSG+
Sbjct: 167 RSKLKSGV 174
>XP_006710244
          Length = 230

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSG+
Sbjct: 167 RSKLKSGV 174
>NP_001243000
          Length = 5207

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RSKLKSGIV 9
           RSKLK+G++
Sbjct: 617 RSKLKTGLI 625
>NP_066005
          Length = 1063

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RSKLKSGIV 9
           RSKLK+G++
Sbjct: 617 RSKLKTGLI 625
>XP_005257603
          Length = 5256

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RSKLKSGIV 9
           RSKLK+G++
Sbjct: 666 RSKLKTGLI 674
>XP_006722058
          Length = 5256

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RSKLKSGIV 9
           RSKLK+G++
Sbjct: 666 RSKLKTGLI 674
>XP_005257602
          Length = 5256

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RSKLKSGIV 9
           RSKLK+G++
Sbjct: 666 RSKLKTGLI 674
>NP_542942
          Length = 543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIV 9
           R+KL+ GI+
Sbjct: 187 RAKLRDGII 195
>XP_006725464
          Length = 543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIV 9
           R+KL+ GI+
Sbjct: 187 RAKLRDGII 195
>XP_006725463
          Length = 543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIV 9
           R+KL+ GI+
Sbjct: 187 RAKLRDGII 195
>XP_005277590
          Length = 900

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIV 9
           RSK++SG +
Sbjct: 151 RSKIRSGCI 159
>XP_005261395
          Length = 543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIV 9
           R+KL+ GI+
Sbjct: 187 RAKLRDGII 195
>NP_000294
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RSKLKSGIV 9
          R K+K G+V
Sbjct: 36 RQKIKIGVV 44
>NP_079133
          Length = 1844

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKLKSG 7
           SKLKSG
Sbjct: 921 SKLKSG 926
>NP_000238
          Length = 383

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RSKLKSGIV 9
           R  LKSG+V
Sbjct: 192 RRYLKSGVV 200
>NP_001334
          Length = 770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SKLKSGI 8
           +KLKSG+
Sbjct: 204 NKLKSGV 210
>NP_006805
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIV 9
           RS++ SGI+
Sbjct: 131 RSRIVSGII 139
>NP_848693
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIV 9
           RS++ SGI+
Sbjct: 131 RSRIVSGII 139
>NP_001231892
          Length = 2488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SKLKSGI 8
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>NP_001136156
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RSKLKSGI 8
           RSK K+G+
Sbjct: 162 RSKTKTGV 169
>NP_660093
          Length = 2429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SKLKSGI 8
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>NP_001034739
          Length = 382

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RSKLKSGI 8
           RSK K+G+
Sbjct: 162 RSKTKTGV 169
>NP_001034738
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RSKLKSGI 8
           RSK K+G+
Sbjct: 162 RSKTKTGV 169
>NP_004843
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKLKSG 7
           SKLKSG
Sbjct: 379 SKLKSG 384
>NP_060480
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKLKSG 7
           SKLKSG
Sbjct: 134 SKLKSG 139
>NP_892009
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SKLKSGI 8
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>NP_004489
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKLKSG 7
           SKLKSG
Sbjct: 338 SKLKSG 343
>XP_006723727
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIV 9
           RS++ SGI+
Sbjct: 131 RSRIVSGII 139
>XP_005260931
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIV 9
           RS++ SGI+
Sbjct: 131 RSRIVSGII 139
>XP_005260930
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIV 9
           RS++ SGI+
Sbjct: 131 RSRIVSGII 139
>XP_005255429
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RSKLKSGIV 9
          R K+K G+V
Sbjct: 36 RQKIKIGVV 44
>XP_005267824
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           +SKL+SG+
Sbjct: 497 KSKLESGL 504
>XP_005266651
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RSKLKSGI 8
           RSK K+G+
Sbjct: 162 RSKTKTGV 169
>XP_006719957
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RSKLKSGI 8
           RSK K+G+
Sbjct: 162 RSKTKTGV 169
>XP_005266650
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RSKLKSGI 8
           RSK K+G+
Sbjct: 162 RSKTKTGV 169
>XP_005266578
          Length = 1290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RSKLKSGI 8
           RSK K+G+
Sbjct: 162 RSKTKTGV 169
>XP_006718428
          Length = 2455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SKLKSGI 8
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>XP_006718427
          Length = 2465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SKLKSGI 8
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>NP_059133
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           +SKL+SG+
Sbjct: 497 KSKLESGL 504
>NP_001073957
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKLKSG 7
           SKLKSG
Sbjct: 367 SKLKSG 372
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.132    0.319 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 467,344
Number of extensions: 670
Number of successful extensions: 50
Number of sequences better than 10000.0: 50
Number of HSP's gapped: 50
Number of HSP's successfully gapped: 50
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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