Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     869
XP_006716142                                                           19     869
XP_005250088                                                           19     869
XP_006716141                                                           19     869
XP_005250085                                                           19     869
XP_006716140                                                           19     869
XP_005250084                                                           19     869
XP_005250083                                                           19     869
XP_005250082                                                           19     869
XP_006726491                                                           18    1135
XP_006715877                                                           18    1135
XP_006710244                                                           18    1135
NP_001136156                                                           17    2528
NP_001034739                                                           17    2528
NP_001034738                                                           17    2528
XP_005266651                                                           17    2528
XP_006719957                                                           17    2528
XP_005266650                                                           17    2528
NP_001243000                                                           17    3301
NP_066005                                                              17    3301
XP_005257603                                                           17    3301
XP_006722058                                                           17    3301
XP_005257602                                                           17    3301
NP_001001662                                                           17    4312
XP_005251799                                                           17    4312
XP_005251801                                                           17    4312
XP_006717044                                                           17    4312
XP_006717043                                                           17    4312
NP_001164218                                                           16    5631
NP_006214                                                              16    5631
NP_060228                                                              16    5631
NP_003449                                                              16    7355
NP_570129                                                              16    7355
XP_006713443                                                           16    7355
XP_006712321                                                           16    7355
NP_079133                                                              15    9605
NP_001334                                                              15    9605
NP_001231892                                                           15    9605
NP_054777                                                              15    9605
NP_660093                                                              15    9605
NP_004843                                                              15    9605
NP_060480                                                              15    9605
NP_892009                                                              15    9605
NP_004489                                                              15    9605
XP_005277590                                                           15    9605
XP_005267824                                                           15    9605
XP_005266578                                                           15    9605
XP_006718428                                                           15    9605
XP_006718427                                                           15    9605
NP_059133                                                              15    9605
NP_001073957                                                           15    9605
>NP_733751
          Length = 4911

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 909 RSKLKSGI 916
>XP_006716142
          Length = 4927

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_005250088
          Length = 4928

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_006716141
          Length = 4959

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_005250085
          Length = 4979

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_006716140
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_005250084
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_005250083
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 909 RSKLKSGI 916
>XP_005250082
          Length = 4983

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSGI
Sbjct: 910 RSKLKSGI 917
>XP_006726491
          Length = 230

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSG+
Sbjct: 167 RSKLKSGV 174
>XP_006715877
          Length = 230

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSG+
Sbjct: 167 RSKLKSGV 174
>XP_006710244
          Length = 230

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLKSG+
Sbjct: 167 RSKLKSGV 174
>NP_001136156
          Length = 669

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIR 9
           RSK K+G+R
Sbjct: 162 RSKTKTGVR 170
>NP_001034739
          Length = 382

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIR 9
           RSK K+G+R
Sbjct: 162 RSKTKTGVR 170
>NP_001034738
          Length = 208

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIR 9
           RSK K+G+R
Sbjct: 162 RSKTKTGVR 170
>XP_005266651
          Length = 669

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIR 9
           RSK K+G+R
Sbjct: 162 RSKTKTGVR 170
>XP_006719957
          Length = 669

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIR 9
           RSK K+G+R
Sbjct: 162 RSKTKTGVR 170
>XP_005266650
          Length = 669

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIR 9
           RSK K+G+R
Sbjct: 162 RSKTKTGVR 170
>NP_001243000
          Length = 5207

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLK+G+
Sbjct: 617 RSKLKTGL 624
>NP_066005
          Length = 1063

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLK+G+
Sbjct: 617 RSKLKTGL 624
>XP_005257603
          Length = 5256

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLK+G+
Sbjct: 666 RSKLKTGL 673
>XP_006722058
          Length = 5256

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLK+G+
Sbjct: 666 RSKLKTGL 673
>XP_005257602
          Length = 5256

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           RSKLK+G+
Sbjct: 666 RSKLKTGL 673
>NP_001001662
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLKSGIR 9
           KLKSG+R
Sbjct: 516 KLKSGLR 522
>XP_005251799
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLKSGIR 9
           KLKSG+R
Sbjct: 516 KLKSGLR 522
>XP_005251801
          Length = 675

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLKSGIR 9
           KLKSG+R
Sbjct: 492 KLKSGLR 498
>XP_006717044
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLKSGIR 9
           KLKSG+R
Sbjct: 516 KLKSGLR 522
>XP_006717043
          Length = 711

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KLKSGIR 9
           KLKSG+R
Sbjct: 528 KLKSGLR 534
>NP_001164218
          Length = 133

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KLKSGIR 9
          KLKSG+R
Sbjct: 82 KLKSGMR 88
>NP_006214
          Length = 156

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KLKSGIR 9
          KLKSG+R
Sbjct: 82 KLKSGMR 88
>NP_060228
          Length = 394

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KLKSGIR 9
          K+KSGIR
Sbjct: 84 KVKSGIR 90
>NP_003449
          Length = 3926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KLKSGIR 9
           K+KSG+R
Sbjct: 634 KVKSGVR 640
>NP_570129
          Length = 1306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIR 9
           R+K+ SG+R
Sbjct: 945 RAKVTSGVR 953
>XP_006713443
          Length = 3970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KLKSGIR 9
           K+KSG+R
Sbjct: 678 KVKSGVR 684
>XP_006712321
          Length = 1307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RSKLKSGIR 9
           R+K+ SG+R
Sbjct: 946 RAKVTSGVR 954
>NP_079133
          Length = 1844

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKLKSG 7
           SKLKSG
Sbjct: 921 SKLKSG 926
>NP_001334
          Length = 770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SKLKSGI 8
           +KLKSG+
Sbjct: 204 NKLKSGV 210
>NP_001231892
          Length = 2488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SKLKSGI 8
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>NP_054777
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SKLKSGIR 9
           SK+K G+R
Sbjct: 254 SKMKRGLR 261
>NP_660093
          Length = 2429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SKLKSGI 8
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>NP_004843
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKLKSG 7
           SKLKSG
Sbjct: 379 SKLKSG 384
>NP_060480
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKLKSG 7
           SKLKSG
Sbjct: 134 SKLKSG 139
>NP_892009
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SKLKSGI 8
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>NP_004489
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKLKSG 7
           SKLKSG
Sbjct: 338 SKLKSG 343
>XP_005277590
          Length = 900

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RSKLKSG 7
           RSK++SG
Sbjct: 151 RSKIRSG 157
>XP_005267824
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           +SKL+SG+
Sbjct: 497 KSKLESGL 504
>XP_005266578
          Length = 1290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RSKLKSGI 8
           RSK K+G+
Sbjct: 162 RSKTKTGV 169
>XP_006718428
          Length = 2455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SKLKSGI 8
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>XP_006718427
          Length = 2465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SKLKSGI 8
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>NP_059133
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RSKLKSGI 8
           +SKL+SG+
Sbjct: 497 KSKLESGL 504
>NP_001073957
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SKLKSG 7
           SKLKSG
Sbjct: 367 SKLKSG 372
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.132    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 446,090
Number of extensions: 648
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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