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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              19     869
NP_653080                                                              19     869
XP_005267292                                                           19     869
XP_005267291                                                           19     869
XP_005267290                                                           19     869
NP_071921                                                              17    4312
NP_001012398                                                           17    4312
XP_005256155                                                           17    4312
XP_005256154                                                           17    4312
XP_005256153                                                           17    4312
XP_005256152                                                           17    4312
XP_005256151                                                           17    4312
NP_060193                                                              16    5631
NP_001273359                                                           16    5631
NP_620147                                                              16    5631
NP_596870                                                              16    7355
NP_659417                                                              16    7355
XP_006715416                                                           16    7355
XP_006715415                                                           16    7355
XP_005266895                                                           16    7355

>NP_005091
          Length = 1782

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SEASEKTM 9
            SEASEKTM
Sbjct: 1635 SEASEKTM 1642



>NP_653080
          Length = 1684

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SEASEKTM 9
            SEASEKTM
Sbjct: 1537 SEASEKTM 1544



>XP_005267292
          Length = 1677

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SEASEKTM 9
            SEASEKTM
Sbjct: 1530 SEASEKTM 1537



>XP_005267291
          Length = 1684

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SEASEKTM 9
            SEASEKTM
Sbjct: 1537 SEASEKTM 1544



>XP_005267290
          Length = 1782

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SEASEKTM 9
            SEASEKTM
Sbjct: 1635 SEASEKTM 1642



>NP_071921
          Length = 292

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RSEASEKTM 9
          RSE  EKT+
Sbjct: 15 RSEGEEKTL 23



>NP_001012398
          Length = 292

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RSEASEKTM 9
          RSE  EKT+
Sbjct: 15 RSEGEEKTL 23



>XP_005256155
          Length = 293

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RSEASEKTM 9
          RSE  EKT+
Sbjct: 15 RSEGEEKTL 23



>XP_005256154
          Length = 293

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RSEASEKTM 9
          RSE  EKT+
Sbjct: 15 RSEGEEKTL 23



>XP_005256153
          Length = 293

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RSEASEKTM 9
          RSE  EKT+
Sbjct: 15 RSEGEEKTL 23



>XP_005256152
          Length = 293

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RSEASEKTM 9
          RSE  EKT+
Sbjct: 15 RSEGEEKTL 23



>XP_005256151
          Length = 293

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RSEASEKTM 9
          RSE  EKT+
Sbjct: 15 RSEGEEKTL 23



>NP_060193
          Length = 423

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RSEASEKT 8
           R+EA EKT
Sbjct: 253 RAEAEEKT 260



>NP_001273359
          Length = 606

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  RSEASEKT 8
          RSEASE++
Sbjct: 87 RSEASERS 94



>NP_620147
          Length = 666

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  RSEASEKT 8
          RSEASE++
Sbjct: 87 RSEASERS 94



>NP_596870
          Length = 5604

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RSEASEK 7
            RSEAS+K
Sbjct: 3733 RSEASDK 3739



>NP_659417
          Length = 469

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RSEASEK 7
           RSE+SEK
Sbjct: 372 RSESSEK 378



>XP_006715416
          Length = 501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RSEASEK 7
           RSE+SEK
Sbjct: 372 RSESSEK 378



>XP_006715415
          Length = 501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RSEASEK 7
           RSE+SEK
Sbjct: 372 RSESSEK 378



>XP_005266895
          Length = 501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RSEASEK 7
           RSE+SEK
Sbjct: 372 RSESSEK 378



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.283   0.0937    0.201 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 326,404
Number of extensions: 432
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)