Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              20     509
XP_005261949                                                           17    4312
XP_005275966                                                           17    4312
NP_079291                                                              16    7355
NP_005301                                                              16    7355
NP_005059                                                              16    7355
NP_001229371                                                           16    7355
NP_443202                                                              16    7355
NP_001184256                                                           16    7355
NP_001025173                                                           16    7355
NP_001229372                                                           16    7355
XP_005260713                                                           16    7355
XP_006721907                                                           16    7355
XP_006717810                                                           16    7355
XP_006717809                                                           16    7355
XP_005269730                                                           16    7355
XP_005269729                                                           16    7355
XP_005267157                                                           16    7355
NP_001137300                                                           15    9605
NP_067637                                                              15    9605
NP_001138600                                                           15    9605
NP_005975                                                              15    9605
NP_001243463                                                           15    9605
NP_060897                                                              15    9605
XP_006715439                                                           15    9605
NP_001078844                                                           15    9605
NP_001128712                                                           15    9605
>NP_073713
          Length = 985

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RRSSLMYGT 9
          RRSSL YGT
Sbjct: 37 RRSSLTYGT 45
>XP_005261949
          Length = 210

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RSSLMYGT 9
           RSS +YGT
Sbjct: 187 RSSTIYGT 194
>XP_005275966
          Length = 210

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RSSLMYGT 9
           RSS +YGT
Sbjct: 187 RSSTIYGT 194
>NP_079291
          Length = 675

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RRSSLMYG 8
           RRS L YG
Sbjct: 531 RRSPLTYG 538
>NP_005301
          Length = 532

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RRSSLMYGT 9
           RRS L Y T
Sbjct: 254 RRSGLYYST 262
>NP_005059
          Length = 766

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RSSLMYG 8
           RSSL YG
Sbjct: 442 RSSLCYG 448
>NP_001229371
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RRSSLMYGT 9
           RRS L Y T
Sbjct: 277 RRSGLYYST 285
>NP_443202
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RRSSLMYG 8
           RRS L YG
Sbjct: 537 RRSPLTYG 544
>NP_001184256
          Length = 685

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RRSSLMYG 8
           RRS L YG
Sbjct: 536 RRSPLTYG 543
>NP_001025173
          Length = 532

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RRSSLMYGT 9
           RRS L Y T
Sbjct: 254 RRSGLYYST 262
>NP_001229372
          Length = 532

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RRSSLMYGT 9
           RRS L Y T
Sbjct: 254 RRSGLYYST 262
>XP_005260713
          Length = 600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RRSSLMYG 8
           RRS L YG
Sbjct: 451 RRSPLTYG 458
>XP_006721907
          Length = 447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RRSSLMYGT 9
           RRS L Y T
Sbjct: 254 RRSGLYYST 262
>XP_006717810
          Length = 691

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RRSSLMYG 8
           RRS L YG
Sbjct: 547 RRSPLTYG 554
>XP_006717809
          Length = 691

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RRSSLMYG 8
           RRS L YG
Sbjct: 547 RRSPLTYG 554
>XP_005269730
          Length = 691

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RRSSLMYG 8
           RRS L YG
Sbjct: 547 RRSPLTYG 554
>XP_005269729
          Length = 692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RRSSLMYG 8
           RRS L YG
Sbjct: 548 RRSPLTYG 555
>XP_005267157
          Length = 766

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RSSLMYG 8
           RSSL YG
Sbjct: 442 RSSLCYG 448
>NP_001137300
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RRSSLMYGT 9
           R+SSLM+ +
Sbjct: 167 RKSSLMFSS 175
>NP_067637
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RRSSLMYGT 9
           R+SSLM+ +
Sbjct: 255 RKSSLMFSS 263
>NP_001138600
          Length = 1911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    RRSSLMY 7
            RRS+L+Y
Sbjct: 1832 RRSALLY 1838
>NP_005975
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SSLMYGT 9
          SSL+YG+
Sbjct: 88 SSLLYGS 94
>NP_001243463
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SSLMYGT 9
           SSL+YG+
Sbjct: 95  SSLLYGS 101
>NP_060897
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RSSLMYGT 9
           RSS+MY T
Sbjct: 96  RSSIMYLT 103
>XP_006715439
          Length = 1911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    RRSSLMY 7
            RRS+L+Y
Sbjct: 1832 RRSALLY 1838
>NP_001078844
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSLMYGT 9
           SSL YGT
Sbjct: 146 SSLEYGT 152
>NP_001128712
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSLMYGT 9
           SSL YGT
Sbjct: 189 SSLEYGT 195
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.129    0.354 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 376,214
Number of extensions: 294
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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