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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_689952                                                              17    2528
NP_775882                                                              17    4312
NP_076922                                                              16    5631
NP_055028                                                              16    5631
XP_006717897                                                           16    5631
XP_006717896                                                           16    5631
NP_114153                                                              16    5631
NP_008828                                                              16    5631
NP_060606                                                              15    9605
NP_067545                                                              15    9605
NP_001034553                                                           15    9605
NP_001497                                                              15    9605
NP_071433                                                              15    9605

>NP_689952
          Length = 272

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RRMMMKKI 8
           RRM MKKI
Sbjct: 257 RRMKMKKI 264



>NP_775882
          Length = 433

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RRMMMKKI 8
           RRM+MK++
Sbjct: 222 RRMLMKRL 229



>NP_076922
          Length = 250

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RRMMMKKI 8
           RRM MKK+
Sbjct: 236 RRMKMKKM 243



>NP_055028
          Length = 352

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RRMMMKKI 8
           RRM MKK+
Sbjct: 336 RRMKMKKM 343



>XP_006717897
          Length = 1160

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RRMMMKKIM 9
           RR++MK+I+
Sbjct: 518 RRLIMKRIV 526



>XP_006717896
          Length = 1186

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RRMMMKKIM 9
           RR++MK+I+
Sbjct: 544 RRLIMKRIV 552



>NP_114153
          Length = 301

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RRMMMKKI 8
           R+MMMK++
Sbjct: 291 RKMMMKQL 298



>NP_008828
          Length = 260

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RRMMMKKI 8
           RRM MKK+
Sbjct: 243 RRMKMKKM 250



>NP_060606
          Length = 3477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    RRMMMKK 7
            RR+M+KK
Sbjct: 2469 RRLMVKK 2475



>NP_067545
          Length = 254

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RRMMMKKIM 9
           RRM  KKI+
Sbjct: 193 RRMKWKKIV 201



>NP_001034553
          Length = 1323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RRMMMKKIM 9
           ++ M+KKIM
Sbjct: 455 KKTMIKKIM 463



>NP_001497
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RRMMMKKI 8
           RR+M+K+I
Sbjct: 282 RRVMLKEI 289



>NP_071433
          Length = 196

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RRMMMKKI 8
           RR++ KKI
Sbjct: 180 RRLLFKKI 187



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.360    0.143    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 289,689
Number of extensions: 249
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.3 bits)
S2: 28 (15.4 bits)