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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              18    1135
XP_006715292                                                           18    1135
NP_006368                                                              16    5631
XP_006716779                                                           16    5631
NP_001166969                                                           15    9605
NP_000519                                                              15    9605
NP_001849                                                              15    9605
XP_005259970                                                           15    9605

>NP_115927
          Length = 1321

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RQTAAMFRV 9
           RQTAAMF V
Sbjct: 332 RQTAAMFGV 340



>XP_006715292
          Length = 759

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RQTAAMFRV 9
           RQTAAMF V
Sbjct: 319 RQTAAMFGV 327



>NP_006368
          Length = 1591

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    QTAAMFR 8
            QTA MFR
Sbjct: 1453 QTAGMFR 1459



>XP_006716779
          Length = 2399

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    QTAAMFR 8
            QTA MFR
Sbjct: 2261 QTAGMFR 2267



>NP_001166969
          Length = 1010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QTAAMFRV 9
           QTAA F+V
Sbjct: 504 QTAARFQV 511



>NP_000519
          Length = 1011

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QTAAMFRV 9
           QTAA F+V
Sbjct: 505 QTAARFQV 512



>NP_001849
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AAMFRV 9
           AAMFRV
Sbjct: 103 AAMFRV 108



>XP_005259970
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QTAAMFRV 9
           QTAA F+V
Sbjct: 506 QTAARFQV 513



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.347    0.132    0.354 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 212,529
Number of extensions: 151
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)