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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              18    1135
NP_055987                                                              17    4312
XP_005245087                                                           17    4312
XP_005245086                                                           17    4312
XP_005245085                                                           17    4312
XP_005245084                                                           17    4312
XP_006711302                                                           17    4312
XP_005245083                                                           17    4312
XP_005245082                                                           17    4312
XP_005245081                                                           17    4312
XP_005245080                                                           17    4312
XP_005245079                                                           17    4312
XP_005245077                                                           17    4312
XP_005245076                                                           17    4312
XP_006711301                                                           17    4312
XP_005245075                                                           17    4312
XP_005245074                                                           17    4312
XP_005245073                                                           17    4312
XP_005245072                                                           17    4312
NP_001241872                                                           16    7355
NP_001129069                                                           16    7355
NP_060554                                                              16    7355
XP_006713245                                                           16    7355
XP_006713243                                                           16    7355
XP_006713242                                                           16    7355
XP_005264484                                                           16    7355
XP_005264483                                                           16    7355
XP_005264480                                                           16    7355
XP_005264478                                                           16    7355
XP_005264475                                                           16    7355
NP_775899                                                              15    9605

>NP_115803
          Length = 1156

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQQSSISAQ 9
           +QQSSISAQ
Sbjct: 619 QQQSSISAQ 627




 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQQSSISAQ 9
           +QQSSISAQ
Sbjct: 631 QQQSSISAQ 639



>NP_055987
          Length = 2817

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 450 RQQSEISA 457



>XP_005245087
          Length = 2708

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_005245086
          Length = 2714

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_005245085
          Length = 2718

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_005245084
          Length = 2719

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_006711302
          Length = 2754

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_005245083
          Length = 2774

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_005245082
          Length = 2779

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_005245081
          Length = 2819

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_005245080
          Length = 2824

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_005245079
          Length = 2838

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_005245077
          Length = 2858

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_005245076
          Length = 2869

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_006711301
          Length = 2896

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 450 RQQSEISA 457



>XP_005245075
          Length = 2898

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 447 RQQSEISA 454



>XP_005245074
          Length = 2898

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>XP_005245073
          Length = 2901

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 450 RQQSEISA 457



>XP_005245072
          Length = 2903

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RQQSSISA 8
           RQQS ISA
Sbjct: 452 RQQSEISA 459



>NP_001241872
          Length = 1796

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQQSSISAQ 9
           RQ+S ISA+
Sbjct: 571 RQRSQISAE 579



>NP_001129069
          Length = 1870

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQQSSISAQ 9
           RQ+S ISA+
Sbjct: 571 RQRSQISAE 579



>NP_060554
          Length = 1843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQQSSISAQ 9
           RQ+S ISA+
Sbjct: 571 RQRSQISAE 579



>XP_006713245
          Length = 1174

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    RQQSSISAQ 9
            R+QSS+++Q
Sbjct: 1127 RRQSSVTSQ 1135



>XP_006713243
          Length = 1199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    RQQSSISAQ 9
            R+QSS+++Q
Sbjct: 1158 RRQSSVTSQ 1166



>XP_006713242
          Length = 1205

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    RQQSSISAQ 9
            R+QSS+++Q
Sbjct: 1158 RRQSSVTSQ 1166



>XP_005264484
          Length = 955

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQQSSISAQ 9
           RQ+S ISA+
Sbjct: 571 RQRSQISAE 579



>XP_005264483
          Length = 1311

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQQSSISAQ 9
           RQ+S ISA+
Sbjct: 571 RQRSQISAE 579



>XP_005264480
          Length = 1842

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQQSSISAQ 9
           RQ+S ISA+
Sbjct: 571 RQRSQISAE 579



>XP_005264478
          Length = 1844

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQQSSISAQ 9
           RQ+S ISA+
Sbjct: 571 RQRSQISAE 579



>XP_005264475
          Length = 2027

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQQSSISAQ 9
           RQ+S ISA+
Sbjct: 571 RQRSQISAE 579



>NP_775899
          Length = 4462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RQQSSISA 8
            +QQ SISA
Sbjct: 1237 KQQKSISA 1244



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.294   0.0973    0.203 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 278,334
Number of extensions: 271
Number of successful extensions: 32
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)