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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              20     509
NP_001035957                                                           20     509
XP_006721991                                                           20     509
XP_006721990                                                           20     509
XP_006721989                                                           20     509
XP_005258041                                                           20     509
XP_006721988                                                           20     509
XP_006721987                                                           20     509
XP_006721986                                                           20     509
XP_005258040                                                           20     509
XP_006721985                                                           20     509
NP_001277114                                                           17    4312
NP_068375                                                              17    4312
XP_005249326                                                           17    4312
XP_006715218                                                           17    4312
NP_068762                                                              17    4312
NP_005742                                                              16    5631
NP_671714                                                              16    5631
NP_001138570                                                           16    5631
NP_001234937                                                           16    5631
NP_001138569                                                           16    5631
NP_005405                                                              16    5631
XP_006715890                                                           16    5631
XP_005250160                                                           16    5631
XP_005250159                                                           16    5631
XP_006713798                                                           16    5631
XP_005247778                                                           16    5631
NP_068351                                                              16    7355
NP_002496                                                              16    7355
NP_997320                                                              15    9605
XP_005253612                                                           15    9605
NP_001001711                                                           15    9605

>NP_000258
          Length = 2818

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQAQTKLEM 9
           RQAQTKLE+
Sbjct: 681 RQAQTKLEV 689



>NP_001035957
          Length = 2839

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQAQTKLEM 9
           RQAQTKLE+
Sbjct: 681 RQAQTKLEV 689



>XP_006721991
          Length = 1632

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQAQTKLEM 9
           RQAQTKLE+
Sbjct: 691 RQAQTKLEV 699



>XP_006721990
          Length = 2823

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQAQTKLEM 9
           RQAQTKLE+
Sbjct: 691 RQAQTKLEV 699



>XP_006721989
          Length = 2823

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQAQTKLEM 9
           RQAQTKLE+
Sbjct: 691 RQAQTKLEV 699



>XP_005258041
          Length = 2836

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQAQTKLEM 9
           RQAQTKLE+
Sbjct: 681 RQAQTKLEV 689



>XP_006721988
          Length = 2846

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQAQTKLEM 9
           RQAQTKLE+
Sbjct: 691 RQAQTKLEV 699



>XP_006721987
          Length = 2849

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQAQTKLEM 9
           RQAQTKLE+
Sbjct: 691 RQAQTKLEV 699



>XP_006721986
          Length = 2854

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQAQTKLEM 9
           RQAQTKLE+
Sbjct: 678 RQAQTKLEV 686



>XP_005258040
          Length = 2857

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQAQTKLEM 9
           RQAQTKLE+
Sbjct: 681 RQAQTKLEV 689



>XP_006721985
          Length = 2867

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RQAQTKLEM 9
           RQAQTKLE+
Sbjct: 691 RQAQTKLEV 699



>NP_001277114
          Length = 931

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RQAQTKLEM 9
           +  QTKLEM
Sbjct: 345 KDTQTKLEM 353



>NP_068375
          Length = 941

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RQAQTKLEM 9
           +  QTKLEM
Sbjct: 355 KDTQTKLEM 363



>XP_005249326
          Length = 205

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QAQTKLEM 9
           Q QTKLE+
Sbjct: 137 QCQTKLEL 144



>XP_006715218
          Length = 218

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QAQTKLEM 9
           Q QTKLE+
Sbjct: 159 QCQTKLEL 166



>NP_068762
          Length = 227

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QAQTKLEM 9
           Q QTKLE+
Sbjct: 159 QCQTKLEL 166



>NP_005742
          Length = 3907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    AQTKLEM 9
            AQTKLE+
Sbjct: 3169 AQTKLEL 3175



>NP_671714
          Length = 3899

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    AQTKLEM 9
            AQTKLE+
Sbjct: 3161 AQTKLEL 3167



>NP_001138570
          Length = 664

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQAQTKLEM 9
           R+A+ KLEM
Sbjct: 638 REARQKLEM 646



>NP_001234937
          Length = 684

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQAQTKLEM 9
           R+A+ KLEM
Sbjct: 658 REARQKLEM 666



>NP_001138569
          Length = 638

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQAQTKLEM 9
           R+A+ KLEM
Sbjct: 612 REARQKLEM 620



>NP_005405
          Length = 684

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQAQTKLEM 9
           R+A+ KLEM
Sbjct: 658 REARQKLEM 666



>XP_006715890
          Length = 3860

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    AQTKLEM 9
            AQTKLE+
Sbjct: 3122 AQTKLEL 3128



>XP_005250160
          Length = 3939

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    AQTKLEM 9
            AQTKLE+
Sbjct: 3201 AQTKLEL 3207



>XP_005250159
          Length = 3952

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    AQTKLEM 9
            AQTKLE+
Sbjct: 3214 AQTKLEL 3220



>XP_006713798
          Length = 684

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQAQTKLEM 9
           R+A+ KLEM
Sbjct: 658 REARQKLEM 666



>XP_005247778
          Length = 684

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RQAQTKLEM 9
           R+A+ KLEM
Sbjct: 658 REARQKLEM 666



>NP_068351
          Length = 318

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RQAQTKLE 8
           RQA+ KLE
Sbjct: 250 RQARAKLE 257



>NP_002496
          Length = 347

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RQAQTKLE 8
           RQA+ KLE
Sbjct: 279 RQARAKLE 286



>NP_997320
          Length = 4471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QAQTKL 7
            QAQTKL
Sbjct: 2128 QAQTKL 2133



>XP_005253612
          Length = 4589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    QAQTKL 7
            QAQTKL
Sbjct: 2246 QAQTKL 2251



>NP_001001711
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RQAQTKLE 8
           R+AQ K+E
Sbjct: 208 REAQAKIE 215



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.115    0.269 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 350,408
Number of extensions: 624
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)