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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_056235                                                              18    1482
NP_055437                                                              17    2528
NP_938057                                                              17    2528
NP_001123614                                                           17    2528
NP_006332                                                              17    4312
XP_006717159                                                           17    4312
XP_005263480                                                           17    4312
NP_001120797                                                           17    4312
NP_055621                                                              16    5631
NP_001129471                                                           16    5631
NP_059984                                                              16    5631
NP_075267                                                              16    5631
XP_006724893                                                           16    5631
XP_006724892                                                           16    5631
XP_005274762                                                           16    5631
NP_068802                                                              16    5631
YP_003024031                                                           16    5631
NP_001035706                                                           16    7355
NP_001186947                                                           16    7355
NP_036323                                                              16    7355
NP_612369                                                              16    7355
NP_001186945                                                           16    7355
XP_005255000                                                           16    7355
XP_005254998                                                           16    7355
NP_001092289                                                           15    9605
NP_000914                                                              15    9605
NP_001092288                                                           15    9605
NP_997297                                                              15    9605
XP_006723232                                                           15    9605

>NP_056235
          Length = 597

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   PIQAFIFT 9
           P++AFIFT
Sbjct: 400 PMEAFIFT 407



>NP_055437
          Length = 786

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PIQAFIFT 9
           P+Q F+FT
Sbjct: 386 PLQLFVFT 393



>NP_938057
          Length = 415

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PIQAFIFT 9
           P+Q F+FT
Sbjct: 386 PLQLFVFT 393



>NP_001123614
          Length = 786

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PIQAFIFT 9
           P+Q F+FT
Sbjct: 386 PLQLFVFT 393



>NP_006332
          Length = 211

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   RPIQAFIF 8
           RP++ F+F
Sbjct: 93  RPVEKFVF 100



>XP_006717159
          Length = 2293

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RPIQAFIFT 9
            RP+ AF+ T
Sbjct: 1610 RPLTAFVIT 1618



>XP_005263480
          Length = 276

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   RPIQAFIF 8
           RP++ F+F
Sbjct: 158 RPVEKFVF 165



>NP_001120797
          Length = 211

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   RPIQAFIF 8
           RP++ F+F
Sbjct: 93  RPVEKFVF 100



>NP_055621
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   PIQAFIF 8
           PIQ F+F
Sbjct: 693 PIQPFVF 699



>NP_001129471
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   PIQAFIF 8
           PIQ F+F
Sbjct: 693 PIQPFVF 699



>NP_059984
          Length = 1871

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RPIQAFIFT 9
          RP +AF+ T
Sbjct: 21 RPFRAFVVT 29



>NP_075267
          Length = 110

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  PIQAFIF 8
          PI AF+F
Sbjct: 85 PISAFVF 91



>XP_006724893
          Length = 1719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RPIQAFIFT 9
          RP +AF+ T
Sbjct: 21 RPFRAFVVT 29



>XP_006724892
          Length = 1841

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RPIQAFIFT 9
          RP +AF+ T
Sbjct: 21 RPFRAFVVT 29



>XP_005274762
          Length = 1853

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RPIQAFIFT 9
          RP +AF+ T
Sbjct: 21 RPFRAFVVT 29



>NP_068802
          Length = 110

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  PIQAFIF 8
          PI AF+F
Sbjct: 85 PISAFVF 91



>YP_003024031
          Length = 226

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IQAFIFT 9
           IQA++FT
Sbjct: 209 IQAYVFT 215



>NP_001035706
          Length = 757

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   PIQAFI 7
           PIQAF+
Sbjct: 290 PIQAFV 295



>NP_001186947
          Length = 286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   PIQAFIFT 9
           P+Q  +FT
Sbjct: 193 PVQGLVFT 200



>NP_036323
          Length = 308

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   PIQAFIFT 9
           P+Q  +FT
Sbjct: 215 PVQGLVFT 222



>NP_612369
          Length = 1372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RPIQAFI 7
           RP+ AFI
Sbjct: 917 RPVSAFI 923



>NP_001186945
          Length = 286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   PIQAFIFT 9
           P+Q  +FT
Sbjct: 193 PVQGLVFT 200



>XP_005255000
          Length = 721

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   PIQAFI 7
           PIQAF+
Sbjct: 290 PIQAFV 295



>XP_005254998
          Length = 757

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   PIQAFI 7
           PIQAF+
Sbjct: 290 PIQAFV 295



>NP_001092289
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RPIQAFIFT 9
           RP+ A IF+
Sbjct: 238 RPLTAIIFS 246



>NP_000914
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RPIQAFIFT 9
           RP+ A IF+
Sbjct: 344 RPLTAIIFS 352



>NP_001092288
          Length = 680

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RPIQAFIFT 9
           RP+ A IF+
Sbjct: 312 RPLTAIIFS 320



>NP_997297
          Length = 1318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PIQAFIFT 9
           P +AF+FT
Sbjct: 284 PEKAFLFT 291



>XP_006723232
          Length = 981

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   RPIQAFIFT 9
           RP+ + +FT
Sbjct: 927 RPLSSLLFT 935



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.348    0.155    0.488 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 463,640
Number of extensions: 731
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)