Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              20     390
NP_001243297                                                           18    1935
NP_689672                                                              16    5631
NP_059984                                                              16    5631
XP_006724893                                                           16    5631
XP_005274763                                                           16    5631
XP_006724892                                                           16    5631
XP_005274762                                                           16    5631
XP_006719241                                                           16    5631
XP_006716240                                                           16    5631
NP_004817                                                              16    7355
NP_001277716                                                           16    7355
NP_001268231                                                           16    7355
NP_115756                                                              16    7355
NP_733792                                                              16    7355
NP_940918                                                              16    7355
NP_733781                                                              16    7355
NP_001254                                                              16    7355
XP_005276467                                                           16    7355
XP_006720086                                                           16    7355
XP_006720085                                                           16    7355
XP_006720084                                                           16    7355
XP_006718287                                                           16    7355
XP_006714784                                                           16    7355
XP_005262744                                                           16    7355
XP_005263976                                                           16    7355
NP_006579                                                              16    7355
NP_001068567                                                           15    9605
NP_003717                                                              15    9605
NP_001714                                                              15    9605
NP_002825                                                              15    9605
NP_001264128                                                           15    9605
NP_054903                                                              15    9605
NP_001264129                                                           15    9605
NP_874358                                                              15    9605
XP_006722869                                                           15    9605
XP_005272548                                                           15    9605
XP_005257813                                                           15    9605
XP_005257814                                                           15    9605
XP_005257812                                                           15    9605
XP_006719590                                                           15    9605
XP_006719589                                                           15    9605
XP_005249383                                                           15    9605
XP_006711831                                                           15    9605
NP_065953                                                              15    9605
NP_001073898                                                           15    9605
>NP_115803
          Length = 1156

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RNVGIMQPT 9
           RNVG MQPT
Sbjct: 806 RNVGNMQPT 814
>NP_001243297
          Length = 102

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  RNVGIMQP 8
          RN+G++QP
Sbjct: 30 RNLGLLQP 37
>NP_689672
          Length = 402

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RNVGIMQP 8
           RN+G +QP
Sbjct: 331 RNLGSLQP 338
>NP_059984
          Length = 1871

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VGIMQP 8
           VG+MQP
Sbjct: 696 VGVMQP 701
>XP_006724893
          Length = 1719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VGIMQP 8
           VG+MQP
Sbjct: 696 VGVMQP 701
>XP_005274763
          Length = 1789

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VGIMQP 8
           VG+MQP
Sbjct: 614 VGVMQP 619
>XP_006724892
          Length = 1841

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VGIMQP 8
           VG+MQP
Sbjct: 696 VGVMQP 701
>XP_005274762
          Length = 1853

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VGIMQP 8
           VG+MQP
Sbjct: 696 VGVMQP 701
>XP_006719241
          Length = 344

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RNVGIMQPT 9
          RN GI+ P+
Sbjct: 51 RNAGILAPS 59
>XP_006716240
          Length = 213

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  RNVGIMQP 8
          RN+G +QP
Sbjct: 15 RNLGSLQP 22
>NP_004817
          Length = 775

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GIMQPT 9
           GI+QPT
Sbjct: 586 GILQPT 591
>NP_001277716
          Length = 773

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GIMQPT 9
           GI+QPT
Sbjct: 584 GILQPT 589
>NP_001268231
          Length = 811

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVGIM 6
           RN+GIM
Sbjct: 103 RNIGIM 108
>NP_115756
          Length = 779

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  RNVGIM 6
          RN+GIM
Sbjct: 71 RNIGIM 76
>NP_733792
          Length = 732

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  RNVGIM 6
          RN+GIM
Sbjct: 71 RNIGIM 76
>NP_940918
          Length = 607

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NVGIMQPT 9
           +VGI+ PT
Sbjct: 515 HVGILSPT 522
>NP_733781
          Length = 513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  RNVGIM 6
          RN+GIM
Sbjct: 71 RNIGIM 76
>NP_001254
          Length = 461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GIMQPT 9
           GI+QPT
Sbjct: 453 GILQPT 458
>XP_005276467
          Length = 97

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  NVGIMQP 8
          N+G++QP
Sbjct: 91 NLGLLQP 97
>XP_006720086
          Length = 703

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   NVGIMQP 8
           N+G++QP
Sbjct: 691 NLGLLQP 697
>XP_006720085
          Length = 704

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   NVGIMQP 8
           N+G++QP
Sbjct: 692 NLGLLQP 698
>XP_006720084
          Length = 730

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   NVGIMQP 8
           N+G++QP
Sbjct: 718 NLGLLQP 724
>XP_006718287
          Length = 545

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NVGIMQPT 9
           +VGI+ PT
Sbjct: 453 HVGILSPT 460
>XP_006714784
          Length = 734

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  RNVGIM 6
          RN+GIM
Sbjct: 71 RNIGIM 76
>XP_005262744
          Length = 421

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   GIMQPT 9
           GI+QPT
Sbjct: 413 GILQPT 418
>XP_005263976
          Length = 227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GIMQPT 9
          GI+QPT
Sbjct: 24 GILQPT 29
>NP_006579
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  GIMQPT 9
          GI+QPT
Sbjct: 24 GILQPT 29
>NP_001068567
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NVGIMQPT 9
           +VGI +PT
Sbjct: 255 SVGIKEPT 262
>NP_003717
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NVGIMQPT 9
           +VGI +PT
Sbjct: 255 SVGIKEPT 262
>NP_001714
          Length = 2649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    RNVGIMQPT 9
            RN G + PT
Sbjct: 1898 RNTGHLHPT 1906
>NP_002825
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NVGIMQ 7
           NVG+MQ
Sbjct: 582 NVGLMQ 587
>NP_001264128
          Length = 76

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2  NVGIMQPT 9
          N+G+  PT
Sbjct: 29 NMGVFDPT 36
>NP_054903
          Length = 139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2  NVGIMQPT 9
          N+G+  PT
Sbjct: 92 NMGVFDPT 99
>NP_001264129
          Length = 137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2  NVGIMQPT 9
          N+G+  PT
Sbjct: 92 NMGVFDPT 99
>NP_874358
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VGIMQPT 9
           VGI++PT
Sbjct: 240 VGIVKPT 246
>XP_006722869
          Length = 1260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    NVGIMQPT 9
            N+G+ +PT
Sbjct: 1073 NLGVKRPT 1080
>XP_005272548
          Length = 1265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    NVGIMQPT 9
            N+G+ +PT
Sbjct: 1078 NLGVKRPT 1085
>XP_005257813
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NVGIMQPT 9
           +VGI +PT
Sbjct: 161 SVGIKEPT 168
>XP_005257814
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NVGIMQPT 9
           +VGI +PT
Sbjct: 161 SVGIKEPT 168
>XP_005257812
          Length = 341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NVGIMQPT 9
           +VGI +PT
Sbjct: 255 SVGIKEPT 262
>XP_006719590
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NVGIMQ 7
           NVG+MQ
Sbjct: 548 NVGLMQ 553
>XP_006719589
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NVGIMQ 7
           NVG+MQ
Sbjct: 586 NVGLMQ 591
>XP_005249383
          Length = 3186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    RNVGIMQPT 9
            RN G + PT
Sbjct: 2435 RNTGHLHPT 2443
>XP_006711831
          Length = 76

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2  NVGIMQPT 9
          N+G+  PT
Sbjct: 29 NMGVFDPT 36
>NP_065953
          Length = 1249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    NVGIMQPT 9
            N+G+ +PT
Sbjct: 1062 NLGVKRPT 1069
>NP_001073898
          Length = 1276

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    NVGIMQPT 9
            N+G+ +PT
Sbjct: 1089 NLGVKRPT 1096
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.136    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 619,951
Number of extensions: 1033
Number of successful extensions: 46
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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