Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              21     229
XP_006715292                                                           21     229
NP_775771                                                              18    1935
XP_006720466                                                           18    1935
XP_005254230                                                           18    1935
XP_006720465                                                           18    1935
XP_005254228                                                           18    1935
NP_055816                                                              17    2528
XP_006714833                                                           17    4312
XP_006714394                                                           17    4312
XP_005263325                                                           17    4312
XP_005263324                                                           17    4312
NP_057308                                                              16    7355
XP_006720386                                                           16    7355
XP_006720385                                                           16    7355
XP_005268275                                                           16    7355
XP_005267072                                                           16    7355
NP_835488                                                              15    9605
NP_073753                                                              15    9605
NP_001073276                                                           15    9605
NP_001073275                                                           15    9605
NP_055039                                                              15    9605
NP_056493                                                              15    9605
NP_005384                                                              15    9605
NP_004541                                                              15    9605
NP_001156729                                                           15    9605
NP_852469                                                              15    9605
NP_005641                                                              15    9605
NP_001159631                                                           15    9605
XP_005276461                                                           15    9605
XP_006725846                                                           15    9605
XP_006725845                                                           15    9605
XP_006725844                                                           15    9605
XP_005277014                                                           15    9605
XP_005277013                                                           15    9605
XP_005277012                                                           15    9605
XP_005261781                                                           15    9605
XP_005261780                                                           15    9605
XP_006724377                                                           15    9605
XP_006724376                                                           15    9605
XP_005261779                                                           15    9605
XP_006719869                                                           15    9605
XP_006719868                                                           15    9605
XP_005266408                                                           15    9605
XP_005266407                                                           15    9605
XP_005266403                                                           15    9605
XP_006719867                                                           15    9605
XP_006719866                                                           15    9605
XP_005266405                                                           15    9605
XP_005266404                                                           15    9605
XP_005265461                                                           15    9605
XP_005245265                                                           15    9605
>NP_115927
          Length = 1321

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RNVAGFRG 8
           RNVAGFRG
Sbjct: 194 RNVAGFRG 201
>XP_006715292
          Length = 759

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RNVAGFRG 8
           RNVAGFRG
Sbjct: 181 RNVAGFRG 188
>NP_775771
          Length = 1244

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RNVAGFRG 8
           R VAGFRG
Sbjct: 181 RAVAGFRG 188
>XP_006720466
          Length = 1175

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RNVAGFRG 8
           R VAGFRG
Sbjct: 112 RAVAGFRG 119
>XP_005254230
          Length = 1175

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RNVAGFRG 8
           R VAGFRG
Sbjct: 112 RAVAGFRG 119
>XP_006720465
          Length = 1239

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RNVAGFRG 8
           R VAGFRG
Sbjct: 176 RAVAGFRG 183
>XP_005254228
          Length = 1250

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RNVAGFRG 8
           R VAGFRG
Sbjct: 187 RAVAGFRG 194
>NP_055816
          Length = 3664

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RNVAGFRG 8
           R V+GFRG
Sbjct: 102 REVSGFRG 109
>XP_006714833
          Length = 774

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RNVAGFRGM 9
           R++AG+RG+
Sbjct: 766 RSLAGWRGL 774
>XP_006714394
          Length = 960

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RNVAGFRG 8
          R V+GFRG
Sbjct: 24 RPVSGFRG 31
>XP_005263325
          Length = 970

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RNVAGFRG 8
          R V+GFRG
Sbjct: 24 RPVSGFRG 31
>XP_005263324
          Length = 1004

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RNVAGFRG 8
          R V+GFRG
Sbjct: 24 RPVSGFRG 31
>NP_057308
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RNVAGFRGM 9
          RN+ G RG+
Sbjct: 43 RNIKGERGL 51
>XP_006720386
          Length = 1571

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   NVAGFRG 8
           NVA FRG
Sbjct: 257 NVATFRG 263
>XP_006720385
          Length = 1683

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   NVAGFRG 8
           NVA FRG
Sbjct: 257 NVATFRG 263
>XP_005268275
          Length = 1684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   NVAGFRG 8
           NVA FRG
Sbjct: 261 NVATFRG 267
>XP_005267072
          Length = 588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RNVAGFRGM 9
          RN+ G RG+
Sbjct: 36 RNIKGERGL 44
>NP_835488
          Length = 343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 204 RNVSGF 209
>NP_073753
          Length = 836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 204 RNVSGF 209
>NP_001073276
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VAGFRGM 9
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>NP_001073275
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VAGFRGM 9
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>NP_055039
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VAGFRGM 9
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>NP_056493
          Length = 2946

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    GFRGM 9
            GFRGM
Sbjct: 1306 GFRGM 1310
>NP_005384
          Length = 1909

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RNVAGFRGM 9
           RN+  FRG+
Sbjct: 706 RNLQHFRGL 714
>NP_004541
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RNVAGFRGM 9
          R + GFRG+
Sbjct: 5  RALCGFRGV 13
>NP_001156729
          Length = 1932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RNVAGFRGM 9
           RN+  FRG+
Sbjct: 729 RNLQHFRGL 737
>NP_852469
          Length = 1938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>NP_005641
          Length = 1960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>NP_001159631
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RNVAGFRGM 9
          R + GFRG+
Sbjct: 5  RALCGFRGV 13
>XP_005276461
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VAGFRGM 9
           ++GF+GM
Sbjct: 345 ISGFQGM 351
>XP_006725846
          Length = 1938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>XP_006725845
          Length = 1949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>XP_006725844
          Length = 1960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>XP_005277014
          Length = 1938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>XP_005277013
          Length = 1949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>XP_005277012
          Length = 1960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>XP_005261781
          Length = 1938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>XP_005261780
          Length = 1949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>XP_006724377
          Length = 1960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>XP_006724376
          Length = 1960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>XP_005261779
          Length = 1960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 719 RNVSGF 724
>XP_006719869
          Length = 2943

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    GFRGM 9
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_006719868
          Length = 2938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    GFRGM 9
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266408
          Length = 1934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    GFRGM 9
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266407
          Length = 2641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   GFRGM 9
           GFRGM
Sbjct: 980 GFRGM 984
>XP_005266403
          Length = 2941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    GFRGM 9
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_006719867
          Length = 2961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    GFRGM 9
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_006719866
          Length = 2962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    GFRGM 9
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266405
          Length = 2964

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    GFRGM 9
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005266404
          Length = 2967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    GFRGM 9
            GFRGM
Sbjct: 1306 GFRGM 1310
>XP_005265461
          Length = 770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RNVAGF 6
           RNV+GF
Sbjct: 138 RNVSGF 143
>XP_005245265
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RNVAGFRGM 9
          R + GFRG+
Sbjct: 5  RALCGFRGV 13
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.148    0.456 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 493,269
Number of extensions: 808
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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