Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              21     229
XP_005265800                                                           21     229
XP_006714103                                                           21     229
XP_006714102                                                           21     229
NP_065681                                                              18    1935
NP_066124                                                              18    1935
NP_653267                                                              18    1935
XP_006717999                                                           18    1935
NP_002600                                                              17    2528
XP_005268521                                                           17    2528
NP_004068                                                              17    3301
NP_036286                                                              16    5631
NP_001158095                                                           16    5631
NP_005498                                                              16    5631
NP_004110                                                              16    5631
NP_056505                                                              16    5631
NP_001230242                                                           16    5631
XP_006712484                                                           16    5631
XP_006712483                                                           16    5631
XP_005246521                                                           16    5631
NP_057460                                                              15    9605
NP_001276976                                                           15    9605
NP_001276975                                                           15    9605
NP_001244928                                                           15    9605
NP_570897                                                              15    9605
NP_001193945                                                           15    9605
NP_003322                                                              15    9605
NP_001155238                                                           15    9605
NP_689837                                                              15    9605
NP_776194                                                              15    9605
NP_002840                                                              15    9605
NP_001229306                                                           15    9605
NP_001193944                                                           15    9605
NP_000678                                                              15    9605
NP_001191375                                                           15    9605
NP_060817                                                              15    9605
XP_006724141                                                           15    9605
XP_005260374                                                           15    9605
XP_005260373                                                           15    9605
XP_006721607                                                           15    9605
XP_005256736                                                           15    9605
XP_005271971                                                           15    9605
NP_001191376                                                           15    9605
>NP_006197
          Length = 1089

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RNILLAQGK 9
           RN+LLAQGK
Sbjct: 822 RNVLLAQGK 830
>XP_005265800
          Length = 1089

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RNILLAQGK 9
           RN+LLAQGK
Sbjct: 822 RNVLLAQGK 830
>XP_006714103
          Length = 1111

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RNILLAQGK 9
           RN+LLAQGK
Sbjct: 844 RNVLLAQGK 852
>XP_006714102
          Length = 1114

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RNILLAQGK 9
           RN+LLAQGK
Sbjct: 847 RNVLLAQGK 855
>NP_065681
          Length = 1072

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RNILLAQGK 9
           RNIL+A+G+
Sbjct: 878 RNILVAEGR 886
>NP_066124
          Length = 1114

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RNILLAQGK 9
           RNIL+A+G+
Sbjct: 878 RNILVAEGR 886
>NP_653267
          Length = 4753

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    RNILLAQGK 9
            RNI+ AQGK
Sbjct: 3817 RNIVRAQGK 3825
>XP_006717999
          Length = 1207

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   RNILLAQGK 9
           RNIL+A+G+
Sbjct: 971 RNILVAEGR 979
>NP_002600
          Length = 1106

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RNILLAQGK 9
           RN+L+ +GK
Sbjct: 830 RNVLICEGK 838
>XP_005268521
          Length = 1042

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RNILLAQGK 9
           RN+L+ +GK
Sbjct: 766 RNVLICEGK 774
>NP_004068
          Length = 466

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NILLAQGK 9
           N+LL QGK
Sbjct: 386 NVLLEQGK 393
>NP_036286
          Length = 859

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NILLAQGK 9
           NILL QG+
Sbjct: 576 NILLPQGR 583
>NP_001158095
          Length = 825

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NILLAQGK 9
           NILL QG+
Sbjct: 576 NILLPQGR 583
>NP_005498
          Length = 166

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  RNILLAQGK 9
          +NI+L +GK
Sbjct: 45 KNIILEEGK 53
>NP_004110
          Length = 993

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RNILLAQGK 9
           RN+L+  GK
Sbjct: 815 RNVLVTHGK 823
>NP_056505
          Length = 1315

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RNILLAQG 8
           +NILLA+G
Sbjct: 129 QNILLAKG 136
>NP_001230242
          Length = 1294

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RNILLAQG 8
           +NILLA+G
Sbjct: 129 QNILLAKG 136
>XP_006712484
          Length = 851

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RNILLAQG 8
           +NILLA+G
Sbjct: 129 QNILLAKG 136
>XP_006712483
          Length = 1306

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RNILLAQG 8
           +NILLA+G
Sbjct: 129 QNILLAKG 136
>XP_005246521
          Length = 1315

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RNILLAQG 8
           +NILLA+G
Sbjct: 129 QNILLAKG 136
>NP_057460
          Length = 1170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RNILLAQGK 9
           RN+LLA  K
Sbjct: 924 RNLLLAGAK 932
>NP_001276976
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LLAQGK 9
           LLAQGK
Sbjct: 384 LLAQGK 389
>NP_001276975
          Length = 1300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LLAQGK 9
           LLAQGK
Sbjct: 221 LLAQGK 226
>NP_001244928
          Length = 1211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RNILLAQGK 9
           RN+LLA  K
Sbjct: 965 RNLLLAGAK 973
>NP_570897
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RNILLAQG 8
           RN++L QG
Sbjct: 286 RNLVLKQG 293
>NP_001193945
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RNILLAQG 8
           RN++L QG
Sbjct: 325 RNLVLKQG 332
>NP_003322
          Length = 1187

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RNILLAQ 7
           RNILLA+
Sbjct: 738 RNILLAR 744
>NP_001155238
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RNILLAQGK 9
           R ILLA+G+
Sbjct: 307 RIILLAEGR 315
>NP_689837
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NILLAQG 8
           N+LL QG
Sbjct: 111 NVLLVQG 117
>NP_776194
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RNILLAQG 8
           RNI LA+G
Sbjct: 299 RNISLAKG 306
>NP_002840
          Length = 657

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RNILLAQG 8
           RN++L QG
Sbjct: 531 RNLVLKQG 538
>NP_001229306
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NILLAQG 8
           N+LL QG
Sbjct: 111 NVLLVQG 117
>NP_001193944
          Length = 545

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RNILLAQG 8
           RN++L QG
Sbjct: 419 RNLVLKQG 426
>NP_000678
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RNILLAQGK 9
           R ILLA+G+
Sbjct: 335 RIILLAEGR 343
>NP_001191375
          Length = 200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NILLAQG 8
           N+LLA+G
Sbjct: 131 NLLLAEG 137
>NP_060817
          Length = 200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NILLAQG 8
           N+LLA+G
Sbjct: 131 NLLLAEG 137
>XP_006724141
          Length = 1426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LLAQGK 9
           LLAQGK
Sbjct: 346 LLAQGK 351
>XP_005260374
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RNILLAQGK 9
           R ILLA+G+
Sbjct: 307 RIILLAEGR 315
>XP_005260373
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RNILLAQGK 9
           R ILLA+G+
Sbjct: 307 RIILLAEGR 315
>XP_006721607
          Length = 1130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RNILLAQGK 9
           RN+LLA  K
Sbjct: 924 RNLLLAGAK 932
>XP_005256736
          Length = 1169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RNILLAQGK 9
           RN+LLA  K
Sbjct: 923 RNLLLAGAK 931
>XP_005271971
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   NILLAQG 8
           N+LL QG
Sbjct: 111 NVLLVQG 117
>NP_001191376
          Length = 200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NILLAQG 8
           N+LLA+G
Sbjct: 131 NLLLAEG 137
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.141    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 421,137
Number of extensions: 709
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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