Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     665
NP_671714                                                              19     665
XP_006715890                                                           19     665
XP_005250160                                                           19     665
XP_005250159                                                           19     665
NP_009146                                                              16    7355
XP_006721714                                                           16    7355
NP_000532                                                              15    9605
NP_001161210                                                           15    9605
NP_065176                                                              15    9605
NP_054760                                                              15    9605
NP_062541                                                              15    9605
NP_671494                                                              15    9605
NP_006268                                                              15    9605
NP_006480                                                              15    9605
NP_002506                                                              15    9605
NP_001157782                                                           15    9605
XP_005267764                                                           15    9605
XP_005246157                                                           15    9605
XP_005246156                                                           15    9605
XP_006711674                                                           15    9605
XP_006711673                                                           15    9605
XP_006711672                                                           15    9605
XP_006711671                                                           15    9605
XP_006711670                                                           15    9605
XP_005245629                                                           15    9605
>NP_005742
          Length = 3907

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV SGFAG
Sbjct: 1824 RLVRSGFAG 1832
>NP_671714
          Length = 3899

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV SGFAG
Sbjct: 1824 RLVRSGFAG 1832
>XP_006715890
          Length = 3860

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV SGFAG
Sbjct: 1777 RLVRSGFAG 1785
>XP_005250160
          Length = 3939

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV SGFAG
Sbjct: 1856 RLVRSGFAG 1864
>XP_005250159
          Length = 3952

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV SGFAG
Sbjct: 1856 RLVRSGFAG 1864
>NP_009146
          Length = 485

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RLVMSGFAG 9
          R ++SGF G
Sbjct: 15 RCLLSGFGG 23
>XP_006721714
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  RLVMSGFAG 9
          R ++SGF G
Sbjct: 15 RCLLSGFGG 23
>NP_000532
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RLVMSGFAG 9
           +L +SGF G
Sbjct: 336 KLTVSGFLG 344
>NP_001161210
          Length = 1483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV+SG +G
Sbjct: 1159 RLVLSGPSG 1167
>NP_065176
          Length = 1877

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV+SG +G
Sbjct: 1553 RLVLSGPSG 1561
>NP_054760
          Length = 169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LVMSGFA 8
           +VM+GFA
Sbjct: 118 IVMAGFA 124
>NP_062541
          Length = 1670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LVMSGFAG 9
           L+M GF G
Sbjct: 195 LMMGGFGG 202
>NP_671494
          Length = 1249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LVMSGFAG 9
           L+M GF G
Sbjct: 195 LMMGGFGG 202
>NP_006268
          Length = 1697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LVMSGFAG 9
           L+M GF G
Sbjct: 195 LMMGGFGG 202
>NP_006480
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VMSGFAG 9
           V+SGF G
Sbjct: 279 VLSGFTG 285
>NP_002506
          Length = 507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VMSGFAG 9
           V+SGF G
Sbjct: 303 VLSGFTG 309
>NP_001157782
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   RLVMSGFAG 9
           +  MSGF G
Sbjct: 177 KFFMSGFTG 185
>XP_005267764
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VMSGFAG 9
           V+SGF G
Sbjct: 181 VLSGFTG 187
>XP_005246157
          Length = 239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RLVMSGFAG 9
           +L +SGF G
Sbjct: 202 KLTVSGFLG 210
>XP_005246156
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RLVMSGFAG 9
           +L +SGF G
Sbjct: 336 KLTVSGFLG 344
>XP_006711674
          Length = 1385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV+SG +G
Sbjct: 1061 RLVLSGPSG 1069
>XP_006711673
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV+SG +G
Sbjct: 1225 RLVLSGPSG 1233
>XP_006711672
          Length = 1557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV+SG +G
Sbjct: 1233 RLVLSGPSG 1241
>XP_006711671
          Length = 2153

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV+SG +G
Sbjct: 1829 RLVLSGPSG 1837
>XP_006711670
          Length = 2161

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV+SG +G
Sbjct: 1837 RLVLSGPSG 1845
>XP_005245629
          Length = 2164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RLVMSGFAG 9
            RLV+SG +G
Sbjct: 1840 RLVLSGPSG 1848
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.147    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 388,212
Number of extensions: 354
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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