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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              18    1482
XP_005265800                                                           18    1482
XP_006714103                                                           18    1482
XP_006714102                                                           18    1482
NP_009029                                                              17    4312
NP_001826                                                              17    4312
XP_006724391                                                           17    4312
XP_005261338                                                           17    4312
XP_005277858                                                           17    4312
XP_006724390                                                           17    4312
XP_006724389                                                           17    4312
XP_005277857                                                           17    4312
NP_002406                                                              16    5631
XP_006726603                                                           16    5631
XP_006725215                                                           16    5631
XP_006719239                                                           16    5631
XP_006719237                                                           16    5631
NP_079083                                                              16    7355
NP_068587                                                              15    9605
NP_001264661                                                           15    9605
NP_003725                                                              15    9605
XP_006726806                                                           15    9605
XP_006722322                                                           15    9605
XP_006719964                                                           15    9605
XP_006719963                                                           15    9605
XP_006719962                                                           15    9605
XP_006719961                                                           15    9605
XP_006719960                                                           15    9605
XP_005266656                                                           15    9605
XP_006710095                                                           15    9605
NP_004285                                                              15    9605

>NP_006197
          Length = 1089

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLSADSVYI 9
            RLSADS YI
Sbjct: 1011 RLSADSGYI 1019



>XP_005265800
          Length = 1089

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLSADSVYI 9
            RLSADS YI
Sbjct: 1011 RLSADSGYI 1019



>XP_006714103
          Length = 1111

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLSADSVYI 9
            RLSADS YI
Sbjct: 1033 RLSADSGYI 1041



>XP_006714102
          Length = 1114

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLSADSVYI 9
            RLSADS YI
Sbjct: 1036 RLSADSGYI 1044



>NP_009029
          Length = 1640

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RLSADSVYI 9
           R+SAD++++
Sbjct: 297 RISADTIFV 305



>NP_001826
          Length = 1583

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RLSADSVYI 9
           R+SAD++++
Sbjct: 297 RISADTIFV 305



>XP_006724391
          Length = 1567

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RLSADSVYI 9
           R+SAD++++
Sbjct: 318 RISADTIFV 326



>XP_005261338
          Length = 1570

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RLSADSVYI 9
           R+SAD++++
Sbjct: 318 RISADTIFV 326



>XP_005277858
          Length = 1604

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RLSADSVYI 9
           R+SAD++++
Sbjct: 318 RISADTIFV 326



>XP_006724390
          Length = 1623

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RLSADSVYI 9
           R+SAD++++
Sbjct: 280 RISADTIFV 288



>XP_006724389
          Length = 1653

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RLSADSVYI 9
           R+SAD++++
Sbjct: 318 RISADTIFV 326



>XP_005277857
          Length = 1661

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   RLSADSVYI 9
           R+SAD++++
Sbjct: 318 RISADTIFV 326



>NP_002406
          Length = 115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RLSADSVYI 9
          R+S D VYI
Sbjct: 89 RISPDRVYI 97



>XP_006726603
          Length = 1264

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RLSADSVY 8
           +LSA+SVY
Sbjct: 415 QLSAESVY 422



>XP_006725215
          Length = 1264

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RLSADSVY 8
           +LSA+SVY
Sbjct: 415 QLSAESVY 422



>XP_006719239
          Length = 1066

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RLSADSVY 8
           +LSA+SVY
Sbjct: 630 QLSAESVY 637



>XP_006719237
          Length = 1263

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RLSADSVY 8
           +LSA+SVY
Sbjct: 415 QLSAESVY 422



>NP_079083
          Length = 321

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  SADSVY 8
          SADSVY
Sbjct: 21 SADSVY 26



>NP_068587
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LSADSVYI 9
           LS+D VYI
Sbjct: 408 LSSDLVYI 415



>NP_001264661
          Length = 220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SADSVY 8
           SADS+Y
Sbjct: 176 SADSIY 181



>NP_003725
          Length = 763

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SADSVY 8
           SADS+Y
Sbjct: 719 SADSIY 724



>XP_006726806
          Length = 259

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SADSVY 8
           SADS+Y
Sbjct: 215 SADSIY 220



>XP_006722322
          Length = 259

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SADSVY 8
           SADS+Y
Sbjct: 215 SADSIY 220



>XP_006719964
          Length = 444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RLSADSVY 8
           R+S D VY
Sbjct: 241 RISGDGVY 248



>XP_006719963
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RLSADSVY 8
           R+S D VY
Sbjct: 241 RISGDGVY 248



>XP_006719962
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RLSADSVY 8
           R+S D VY
Sbjct: 241 RISGDGVY 248



>XP_006719961
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RLSADSVY 8
           R+S D VY
Sbjct: 241 RISGDGVY 248



>XP_006719960
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RLSADSVY 8
           R+S D VY
Sbjct: 241 RISGDGVY 248



>XP_005266656
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RLSADSVY 8
           R+S D VY
Sbjct: 241 RISGDGVY 248



>XP_006710095
          Length = 259

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SADSVY 8
           SADS+Y
Sbjct: 215 SADSIY 220



>NP_004285
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RLSADSVY 8
           R+S D VY
Sbjct: 241 RISGDGVY 248



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.133    0.341 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 366,693
Number of extensions: 427
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)