Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              20     299
NP_000811                                                              18    1935
NP_001034568                                                           17    3301
XP_005268301                                                           17    3301
XP_006720407                                                           17    3301
NP_001120652                                                           16    5631
NP_000251                                                              16    5631
NP_001158268                                                           16    5631
NP_579875                                                              16    5631
NP_758847                                                              16    5631
NP_003080                                                              16    5631
NP_775742                                                              16    5631
XP_005259234                                                           16    5631
XP_006718624                                                           16    5631
XP_005274069                                                           16    5631
XP_006718623                                                           16    5631
XP_006718622                                                           16    5631
XP_006718621                                                           16    5631
XP_006713021                                                           16    5631
XP_006713020                                                           16    5631
XP_006713019                                                           16    5631
NP_694972                                                              16    7355
NP_003226                                                              16    7355
NP_001180473                                                           16    7355
NP_001146                                                              16    7355
XP_005251100                                                           16    7355
XP_006716686                                                           16    7355
XP_005251099                                                           16    7355
XP_005251097                                                           16    7355
XP_005251095                                                           16    7355
XP_006716685                                                           16    7355
XP_005268489                                                           16    7355
NP_001166169                                                           15    9605
NP_573570                                                              15    9605
NP_001907                                                              15    9605
NP_001166170                                                           15    9605
NP_689716                                                              15    9605
NP_998797                                                              15    9605
NP_998796                                                              15    9605
XP_005268429                                                           15    9605
>NP_004251
          Length = 1208

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RLRRTFQVF 9
            RLRRTFQ F
Sbjct: 1069 RLRRTFQAF 1077
>NP_000811
          Length = 678

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  RLRRTFQVF 9
          R RR FQVF
Sbjct: 45 RQRRAFQVF 53
>NP_001034568
          Length = 823

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RRTFQVF 9
           R+TFQVF
Sbjct: 238 RQTFQVF 244
>XP_005268301
          Length = 662

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RRTFQVF 9
          R+TFQVF
Sbjct: 77 RQTFQVF 83
>XP_006720407
          Length = 813

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RRTFQVF 9
           R+TFQVF
Sbjct: 228 RQTFQVF 234
>NP_001120652
          Length = 2175

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RLRRTF 6
            RLRRTF
Sbjct: 1230 RLRRTF 1235
>NP_000251
          Length = 2215

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RLRRTF 6
            RLRRTF
Sbjct: 1230 RLRRTF 1235
>NP_001158268
          Length = 186

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  RTFQVF 9
          RTFQ+F
Sbjct: 35 RTFQIF 40
>NP_579875
          Length = 436

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLRRTFQ 7
           R+RRTF+
Sbjct: 156 RMRRTFE 162
>NP_758847
          Length = 425

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RLRRTFQ 7
           R+RRTF+
Sbjct: 156 RMRRTFE 162
>NP_003080
          Length = 437

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RLRRTFQVF 9
           +LRR F+V+
Sbjct: 118 KLRREFEVY 126
>NP_775742
          Length = 274

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RTFQVF 9
           RTFQ+F
Sbjct: 123 RTFQIF 128
>XP_005259234
          Length = 428

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RLRRTFQVF 9
           +LRR F+V+
Sbjct: 118 KLRREFEVY 126
>XP_006718624
          Length = 2177

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RLRRTF 6
            RLRRTF
Sbjct: 1230 RLRRTF 1235
>XP_005274069
          Length = 2176

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RLRRTF 6
            RLRRTF
Sbjct: 1230 RLRRTF 1235
>XP_006718623
          Length = 2189

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RLRRTF 6
            RLRRTF
Sbjct: 1230 RLRRTF 1235
>XP_006718622
          Length = 2190

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RLRRTF 6
            RLRRTF
Sbjct: 1230 RLRRTF 1235
>XP_006718621
          Length = 2225

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    RLRRTF 6
            RLRRTF
Sbjct: 1230 RLRRTF 1235
>XP_006713021
          Length = 161

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RTFQVF 9
           RTFQ+F
Sbjct: 123 RTFQIF 128
>XP_006713020
          Length = 174

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RTFQVF 9
           RTFQ+F
Sbjct: 123 RTFQIF 128
>XP_006713019
          Length = 259

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RTFQVF 9
           RTFQ+F
Sbjct: 123 RTFQIF 128
>NP_694972
          Length = 1330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RLRRTFQVF 9
           RLR+ +++F
Sbjct: 93  RLRKEYEIF 101
>NP_003226
          Length = 2768

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LRRTFQVF 9
            L +TFQVF
Sbjct: 1313 LLQTFQVF 1320
>NP_001180473
          Length = 641

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LRRTFQVF 9
           LRR FQ F
Sbjct: 527 LRRVFQEF 534
>NP_001146
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LRRTFQVF 9
           LRR FQ F
Sbjct: 559 LRRVFQEF 566
>XP_005251100
          Length = 2103

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LRRTFQVF 9
            L +TFQVF
Sbjct: 1313 LLQTFQVF 1320
>XP_006716686
          Length = 2111

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LRRTFQVF 9
            L +TFQVF
Sbjct: 1313 LLQTFQVF 1320
>XP_005251099
          Length = 2121

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LRRTFQVF 9
            L +TFQVF
Sbjct: 1313 LLQTFQVF 1320
>XP_005251097
          Length = 2178

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LRRTFQVF 9
            L +TFQVF
Sbjct: 1313 LLQTFQVF 1320
>XP_005251095
          Length = 2704

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LRRTFQVF 9
            L +TFQVF
Sbjct: 1313 LLQTFQVF 1320
>XP_006716685
          Length = 2747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LRRTFQVF 9
            L +TFQVF
Sbjct: 1313 LLQTFQVF 1320
>XP_005268489
          Length = 667

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LRRTFQVF 9
           LRR FQ F
Sbjct: 553 LRRVFQEF 560
>NP_001166169
          Length = 984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRRTFQVF 9
           L+R F+VF
Sbjct: 879 LKRRFEVF 886
>NP_573570
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRRTFQVF 9
           L+R F+VF
Sbjct: 918 LKRRFEVF 925
>NP_001907
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRRTFQVF 9
           +RR FQV+
Sbjct: 110 IRRGFQVY 117
>NP_001166170
          Length = 959

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRRTFQVF 9
           L+R F+VF
Sbjct: 854 LKRRFEVF 861
>NP_689716
          Length = 1052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RRTFQVF 9
           R+TFQ F
Sbjct: 305 RQTFQAF 311
>NP_998797
          Length = 573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RRTFQVF 9
           R+TFQ F
Sbjct: 305 RQTFQAF 311
>NP_998796
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RRTFQVF 9
           R+TFQ F
Sbjct: 305 RQTFQAF 311
>XP_005268429
          Length = 1002

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRRTFQVF 9
           L+R F+VF
Sbjct: 897 LKRRFEVF 904
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.361    0.159    0.474 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 432,309
Number of extensions: 743
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.3 bits)
S2: 28 (15.4 bits)

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