Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_653165                                                              17    4312
NP_000129                                                              16    5631
NP_001136336                                                           16    5631
XP_006721417                                                           16    5631
XP_006721416                                                           16    5631
NP_003943                                                              16    7355
XP_006721609                                                           16    7355
XP_005274221                                                           16    7355
XP_005246247                                                           16    7355
NP_036525                                                              16    7355
NP_116179                                                              15    9605
NP_060521                                                              15    9605
NP_055529                                                              15    9605
NP_001013712                                                           15    9605
XP_006721055                                                           15    9605
XP_006721054                                                           15    9605
XP_006721053                                                           15    9605
XP_006721052                                                           15    9605
XP_005268927                                                           15    9605
XP_005249607                                                           15    9605
XP_005249606                                                           15    9605
XP_006716104                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    LLELAFAF 9
            LLELAFAF
Sbjct: 1514 LLELAFAF 1521
>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    LLELAFAF 9
            LLELAFAF
Sbjct: 1514 LLELAFAF 1521
>NP_653165
          Length = 314

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RLLELAFA 8
           RLL LAFA
Sbjct: 169 RLLSLAFA 176
>NP_000129
          Length = 2871

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RLLELAFAF 9
          RLLE+A  F
Sbjct: 5  RLLEIALGF 13
>NP_001136336
          Length = 2521

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RLLELAFAF 9
           RLLELA  F
Sbjct: 787 RLLELAAGF 795
>XP_006721417
          Length = 2497

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RLLELAFAF 9
           RLLELA  F
Sbjct: 787 RLLELAAGF 795
>XP_006721416
          Length = 2504

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RLLELAFAF 9
           RLLELA  F
Sbjct: 770 RLLELAAGF 778
>NP_003943
          Length = 482

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LLELAFAF 9
           ++ELA+AF
Sbjct: 131 IVELAYAF 138
>XP_006721609
          Length = 947

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LLELAFA 8
           LLE+AFA
Sbjct: 919 LLEMAFA 925
>XP_005274221
          Length = 385

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  LLELAFAF 9
          ++ELA+AF
Sbjct: 34 IVELAYAF 41
>XP_005246247
          Length = 1417

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RLLELAF 7
            RLL+LAF
Sbjct: 1388 RLLDLAF 1394
>NP_036525
          Length = 1338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LLELAFA 8
           LLE+AFA
Sbjct: 919 LLEMAFA 925
>NP_116179
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RLLELAFAF 9
           R +ELA+AF
Sbjct: 170 RHIELAWAF 178
>NP_060521
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RLLELAFAF 9
           RL++L F+F
Sbjct: 495 RLIDLDFSF 503
>NP_055529
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RLLELAFA 8
            + LELAFA
Sbjct: 1096 KALELAFA 1103
>NP_001013712
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RLLELAFA 8
           RLL+ AFA
Sbjct: 471 RLLQAAFA 478
>XP_006721055
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RLLELAFA 8
           + LELAFA
Sbjct: 307 KALELAFA 314
>XP_006721054
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RLLELAFA 8
            + LELAFA
Sbjct: 1096 KALELAFA 1103
>XP_006721053
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RLLELAFA 8
            + LELAFA
Sbjct: 1096 KALELAFA 1103
>XP_006721052
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RLLELAFA 8
            + LELAFA
Sbjct: 1096 KALELAFA 1103
>XP_005268927
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RLLELAFA 8
           RLL+ AFA
Sbjct: 471 RLLQAAFA 478
>XP_005249607
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  RLLELAFAF 9
          RL++L F+F
Sbjct: 8  RLIDLDFSF 16
>XP_005249606
          Length = 878

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RLLELAFAF 9
           RL++L F+F
Sbjct: 307 RLIDLDFSF 315
>XP_006716104
          Length = 1069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RLLELAFAF 9
           RL++L F+F
Sbjct: 498 RLIDLDFSF 506
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.353    0.159    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 361,390
Number of extensions: 458
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.9 bits)
S2: 28 (15.4 bits)

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