Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     390
NP_001075109                                                           20     390
NP_001278086                                                           17    3301
NP_001181876                                                           17    3301
NP_937887                                                              17    3301
NP_937884                                                              17    3301
NP_004944                                                              17    3301
NP_001181875                                                           17    3301
NP_937885                                                              17    3301
NP_886553                                                              17    3301
NP_064544                                                              17    3301
NP_001241878                                                           17    3301
NP_001185731                                                           16    5631
NP_001185730                                                           16    5631
NP_003751                                                              16    5631
NP_001078823                                                           15    9605
NP_055696                                                              15    9605
NP_001275964                                                           15    9605
NP_001275963                                                           15    9605
NP_612352                                                              15    9605
NP_115667                                                              15    9605
NP_078933                                                              15    9605
NP_001258745                                                           15    9605
NP_001001850                                                           15    9605
NP_958930                                                              15    9605
NP_958929                                                              15    9605
NP_958931                                                              15    9605
XP_006724755                                                           15    9605
XP_006724754                                                           15    9605
XP_005262247                                                           15    9605
XP_006721093                                                           15    9605
XP_006721091                                                           15    9605
XP_006718153                                                           15    9605
XP_005247513                                                           15    9605
XP_005247512                                                           15    9605
XP_005271327                                                           15    9605
XP_005271326                                                           15    9605
XP_006711037                                                           15    9605
XP_005271325                                                           15    9605
XP_006711036                                                           15    9605
XP_006711035                                                           15    9605
XP_006711034                                                           15    9605
XP_005271323                                                           15    9605
XP_006711032                                                           15    9605
XP_006711031                                                           15    9605
XP_006711030                                                           15    9605
XP_006711029                                                           15    9605
XP_005271322                                                           15    9605
XP_006711028                                                           15    9605
XP_006711027                                                           15    9605
XP_006711026                                                           15    9605
XP_005271321                                                           15    9605
XP_006711025                                                           15    9605
XP_006711024                                                           15    9605
>NP_008835
          Length = 4128

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  RKSLNNIEF 9
          RKSLN+IEF
Sbjct: 70 RKSLNSIEF 78
>NP_001075109
          Length = 4097

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  RKSLNNIEF 9
          RKSLN+IEF
Sbjct: 70 RKSLNSIEF 78
>NP_001278086
          Length = 1559

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKSLNNIEF 9
           R+SL NI+F
Sbjct: 853 RRSLGNIKF 861
>NP_001181876
          Length = 1606

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKSLNNIEF 9
           R+SL NI+F
Sbjct: 900 RRSLGNIKF 908
>NP_937887
          Length = 1512

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKSLNNIEF 9
           R+SL NI+F
Sbjct: 806 RRSLGNIKF 814
>NP_937884
          Length = 1599

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKSLNNIEF 9
           R+SL NI+F
Sbjct: 893 RRSLGNIKF 901
>NP_004944
          Length = 1404

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKSLNNIEF 9
           R+SL NI+F
Sbjct: 698 RRSLGNIKF 706
>NP_001181875
          Length = 1606

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKSLNNIEF 9
           R+SL NI+F
Sbjct: 900 RRSLGNIKF 908
>NP_937885
          Length = 1435

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKSLNNIEF 9
           R+SL NI+F
Sbjct: 729 RRSLGNIKF 737
>NP_886553
          Length = 1600

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKSLNNIEF 9
           R+SL NI+F
Sbjct: 894 RRSLGNIKF 902
>NP_064544
          Length = 1026

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RKSLNNIE 8
           +KS+NN+E
Sbjct: 759 KKSINNLE 766
>NP_001241878
          Length = 596

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RKSLNNIE 8
           +KS+NN+E
Sbjct: 329 KKSINNLE 336
>NP_001185731
          Length = 1591

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RKSLNNIEF 9
           R+S+ NI+F
Sbjct: 893 RRSIGNIKF 901
>NP_001185730
          Length = 1621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RKSLNNIEF 9
           R+S+ NI+F
Sbjct: 923 RRSIGNIKF 931
>NP_003751
          Length = 1585

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RKSLNNIEF 9
           R+S+ NI+F
Sbjct: 887 RRSIGNIKF 895
>NP_001078823
          Length = 900

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSLNNIEF 9
           KSL N+E+
Sbjct: 297 KSLANVEY 304
>NP_055696
          Length = 1040

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KSLNNIEF 9
           KSL+++EF
Sbjct: 605 KSLSDLEF 612
>NP_001275964
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  RKSLNNIE 8
          R+S+ NIE
Sbjct: 72 RRSMENIE 79
>NP_001275963
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  RKSLNNIE 8
          R+S+ NIE
Sbjct: 72 RRSMENIE 79
>NP_612352
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  RKSLNNIE 8
          R+S+ NIE
Sbjct: 72 RRSMENIE 79
>NP_115667
          Length = 828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 719 ALNNVQF 725
>NP_078933
          Length = 937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSLNNIEF 9
           KSL N+E+
Sbjct: 297 KSLANVEY 304
>NP_001258745
          Length = 1040

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KSLNNIEF 9
           KSL+++EF
Sbjct: 605 KSLSDLEF 612
>NP_001001850
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KSLNNIE 8
           +S+NNIE
Sbjct: 245 ESINNIE 251
>NP_958930
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  RKSLNNIE 8
          R+S+ NIE
Sbjct: 72 RRSMENIE 79
>NP_958929
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  RKSLNNIE 8
          R+S+ NIE
Sbjct: 72 RRSMENIE 79
>NP_958931
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  RKSLNNIE 8
          R+S+ NIE
Sbjct: 90 RRSMENIE 97
>XP_006724755
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSLNNIEF 9
           KSL N+E+
Sbjct: 297 KSLANVEY 304
>XP_006724754
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSLNNIEF 9
           KSL N+E+
Sbjct: 175 KSLANVEY 182
>XP_005262247
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSLNNIEF 9
           KSL N+E+
Sbjct: 205 KSLANVEY 212
>XP_006721093
          Length = 220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RKSLNNIE 8
           RKS+N+I+
Sbjct: 207 RKSINSIK 214
>XP_006721091
          Length = 280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RKSLNNIE 8
           RKS+N+I+
Sbjct: 267 RKSINSIK 274
>XP_006718153
          Length = 1040

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KSLNNIEF 9
           KSL+++EF
Sbjct: 605 KSLSDLEF 612
>XP_005247513
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KSLNNIE 8
           +S+NNIE
Sbjct: 245 ESINNIE 251
>XP_005247512
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KSLNNIE 8
           +S+NNIE
Sbjct: 245 ESINNIE 251
>XP_005271327
          Length = 629

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 520 ALNNVQF 526
>XP_005271326
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 522 ALNNVQF 528
>XP_006711037
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 522 ALNNVQF 528
>XP_005271325
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 528 ALNNVQF 534
>XP_006711036
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 550 ALNNVQF 556
>XP_006711035
          Length = 665

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 556 ALNNVQF 562
>XP_006711034
          Length = 667

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 558 ALNNVQF 564
>XP_005271323
          Length = 798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 689 ALNNVQF 695
>XP_006711032
          Length = 804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 695 ALNNVQF 701
>XP_006711031
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 697 ALNNVQF 703
>XP_006711030
          Length = 808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 699 ALNNVQF 705
>XP_006711029
          Length = 812

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 703 ALNNVQF 709
>XP_005271322
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 713 ALNNVQF 719
>XP_006711028
          Length = 826

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 717 ALNNVQF 723
>XP_006711027
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 721 ALNNVQF 727
>XP_006711026
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 721 ALNNVQF 727
>XP_005271321
          Length = 832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 723 ALNNVQF 729
>XP_006711025
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 725 ALNNVQF 731
>XP_006711024
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   SLNNIEF 9
           +LNN++F
Sbjct: 725 ALNNVQF 731
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.135    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 399,465
Number of extensions: 636
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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