Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005276380                                                           17    3301
XP_005251180                                                           17    3301
XP_005276078                                                           17    3301
NP_940861                                                              16    7355
NP_001243333                                                           16    7355
NP_653294                                                              16    7355
NP_001273424                                                           16    7355
NP_062546                                                              16    7355
XP_005259813                                                           16    7355
XP_005259812                                                           16    7355
XP_006720675                                                           16    7355
XP_006720674                                                           16    7355
XP_006720672                                                           16    7355
XP_005254605                                                           16    7355
XP_005254604                                                           16    7355
NP_001073991                                                           15    9605
NP_598328                                                              15    9605
NP_000099                                                              15    9605
NP_060136                                                              15    9605
NP_001276680                                                           15    9605
NP_001254708                                                           15    9605
NP_001156863                                                           15    9605
NP_001276679                                                           15    9605
NP_001276681                                                           15    9605
NP_859074                                                              15    9605
NP_001129246                                                           15    9605
NP_001180481                                                           15    9605
NP_008881                                                              15    9605
NP_071904                                                              15    9605
NP_001803                                                              15    9605
NP_065996                                                              15    9605
XP_006724828                                                           15    9605
XP_006723248                                                           15    9605
XP_005258914                                                           15    9605
XP_005258915                                                           15    9605
XP_005266561                                                           15    9605
XP_005266560                                                           15    9605
XP_005266559                                                           15    9605
XP_005270064                                                           15    9605
XP_005270062                                                           15    9605
XP_005270061                                                           15    9605
XP_005273535                                                           15    9605
XP_006716393                                                           15    9605
XP_006716392                                                           15    9605
XP_006716391                                                           15    9605
XP_005273534                                                           15    9605
XP_006715347                                                           15    9605
XP_006715346                                                           15    9605
XP_006715345                                                           15    9605
XP_006714128                                                           15    9605
XP_006714127                                                           15    9605
XP_005248234                                                           15    9605
XP_006713376                                                           15    9605
XP_006713375                                                           15    9605
NP_003481                                                              15    9605
>XP_005276380
          Length = 232

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  RKSFASRS 8
          RKSF SRS
Sbjct: 27 RKSFLSRS 34
>XP_005251180
          Length = 229

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  RKSFASRS 8
          RKSF SRS
Sbjct: 27 RKSFLSRS 34
>XP_005276078
          Length = 232

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  RKSFASRS 8
          RKSF SRS
Sbjct: 27 RKSFLSRS 34
>NP_940861
          Length = 871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RKSFASRSI 9
           RKS  SRS+
Sbjct: 796 RKSHTSRSV 804
>NP_001243333
          Length = 928

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RKSFASRSI 9
           RKS  SRS+
Sbjct: 853 RKSHTSRSV 861
>NP_653294
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           KSF+SRS
Sbjct: 187 KSFSSRS 193
>NP_001273424
          Length = 1083

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           KSF+SRS
Sbjct: 848 KSFSSRS 854
>NP_062546
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           KSF+SRS
Sbjct: 841 KSFSSRS 847
>XP_005259813
          Length = 331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           KSF+SRS
Sbjct: 116 KSFSSRS 122
>XP_005259812
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           KSF+SRS
Sbjct: 187 KSFSSRS 193
>XP_006720675
          Length = 988

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           KSF+SRS
Sbjct: 753 KSFSSRS 759
>XP_006720674
          Length = 988

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           KSF+SRS
Sbjct: 753 KSFSSRS 759
>XP_006720672
          Length = 1053

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           KSF+SRS
Sbjct: 841 KSFSSRS 847
>XP_005254605
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           KSF+SRS
Sbjct: 841 KSFSSRS 847
>XP_005254604
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           KSF+SRS
Sbjct: 841 KSFSSRS 847
>NP_001073991
          Length = 1620

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KSFASRSI 9
            KSF SRS+
Sbjct: 1463 KSFFSRSL 1470
>NP_598328
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RKSFAS 6
           RKSFAS
Sbjct: 573 RKSFAS 578
>NP_000099
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KSFASRSI 9
           K FASR I
Sbjct: 104 KDFASRGI 111
>NP_060136
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RKSFASRS 8
           RKS ASRS
Sbjct: 553 RKSNASRS 560
>NP_001276680
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  KSFASRSI 9
          K FASR I
Sbjct: 81 KDFASRGI 88
>NP_001254708
          Length = 1058

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           KSF SRS
Sbjct: 536 KSFTSRS 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           KSF SRS
Sbjct: 816 KSFTSRS 822
>NP_001156863
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KSFASRSI 9
           KSF+ RSI
Sbjct: 393 KSFSRRSI 400
>NP_001276679
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  KSFASRSI 9
          K FASR I
Sbjct: 5  KDFASRGI 12
>NP_001276681
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KSFASRSI 9
           K FASR I
Sbjct: 104 KDFASRGI 111
>NP_859074
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SFASRSI 9
           +FASRSI
Sbjct: 447 NFASRSI 453
>NP_001129246
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RKSFAS 6
           RKSFAS
Sbjct: 570 RKSFAS 575
>NP_001180481
          Length = 1090

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           KSF SRS
Sbjct: 568 KSFTSRS 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           KSF SRS
Sbjct: 848 KSFTSRS 854
>NP_008881
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RKSFAS 6
           RKSFAS
Sbjct: 696 RKSFAS 701
>NP_071904
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           K FASRS
Sbjct: 731 KQFASRS 737
>NP_001803
          Length = 943

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           +SFASRS
Sbjct: 310 QSFASRS 316
>NP_065996
          Length = 3184

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RKSFASRS 8
            +KS ASRS
Sbjct: 2179 KKSLASRS 2186
>XP_006724828
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   RKSFASRS 8
           RKS ASRS
Sbjct: 504 RKSNASRS 511
>XP_006723248
          Length = 1060

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           KSF SRS
Sbjct: 538 KSFTSRS 544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           KSF SRS
Sbjct: 818 KSFTSRS 824
>XP_005258914
          Length = 1092

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           KSF SRS
Sbjct: 570 KSFTSRS 576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           KSF SRS
Sbjct: 850 KSFTSRS 856
>XP_005258915
          Length = 1092

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           KSF SRS
Sbjct: 570 KSFTSRS 576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           KSF SRS
Sbjct: 850 KSFTSRS 856
>XP_005266561
          Length = 861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           K FASRS
Sbjct: 441 KQFASRS 447
>XP_005266560
          Length = 1037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           K FASRS
Sbjct: 731 KQFASRS 737
>XP_005266559
          Length = 1077

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KSFASRS 8
           K FASRS
Sbjct: 657 KQFASRS 663
>XP_005270064
          Length = 1615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKSFASRS 8
           +KS ASRS
Sbjct: 610 KKSLASRS 617
>XP_005270062
          Length = 3184

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RKSFASRS 8
            +KS ASRS
Sbjct: 2179 KKSLASRS 2186
>XP_005270061
          Length = 3184

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RKSFASRS 8
            +KS ASRS
Sbjct: 2179 KKSLASRS 2186
>XP_005273535
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SFASRSI 9
           +FASRSI
Sbjct: 271 NFASRSI 277
>XP_006716393
          Length = 407

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SFASRSI 9
           +FASRSI
Sbjct: 324 NFASRSI 330
>XP_006716392
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SFASRSI 9
           +FASRSI
Sbjct: 402 NFASRSI 408
>XP_006716391
          Length = 498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SFASRSI 9
           +FASRSI
Sbjct: 415 NFASRSI 421
>XP_005273534
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SFASRSI 9
           +FASRSI
Sbjct: 434 NFASRSI 440
>XP_006715347
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KSFASRSI 9
           KSF+ RSI
Sbjct: 393 KSFSRRSI 400
>XP_006715346
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KSFASRSI 9
           KSF+ RSI
Sbjct: 393 KSFSRRSI 400
>XP_006715345
          Length = 611

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KSFASRSI 9
           KSF+ RSI
Sbjct: 393 KSFSRRSI 400
>XP_006714128
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           +SFASRS
Sbjct: 97  QSFASRS 103
>XP_006714127
          Length = 935

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KSFASRS 8
           +SFASRS
Sbjct: 310 QSFASRS 316
>XP_005248234
          Length = 1620

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KSFASRSI 9
            KSF SRS+
Sbjct: 1463 KSFFSRSL 1470
>XP_006713376
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RKSFAS 6
           RKSFAS
Sbjct: 316 RKSFAS 321
>XP_006713375
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RKSFAS 6
           RKSFAS
Sbjct: 412 RKSFAS 417
>NP_003481
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RKSFAS 6
           RKSFAS
Sbjct: 571 RKSFAS 576
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.123    0.296 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 327,398
Number of extensions: 674
Number of successful extensions: 60
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 60
Number of HSP's successfully gapped: 60
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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