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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_062541                                                              17    4312
NP_006268                                                              17    4312
NP_001229285                                                           16    7355
NP_001229286                                                           16    7355
NP_542376                                                              16    7355
NP_004361                                                              16    7355
XP_006715397                                                           16    7355

>NP_062541
          Length = 1670

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKSAQFQMY 9
            + +AQF+MY
Sbjct: 1454 KSNAQFKMY 1462



>NP_006268
          Length = 1697

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKSAQFQMY 9
            + +AQF+MY
Sbjct: 1481 KSNAQFKMY 1489



>NP_001229285
          Length = 209

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  RKSAQFQMY 9
          R SAQ QMY
Sbjct: 18 RISAQQQMY 26



>NP_001229286
          Length = 192

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  RKSAQFQMY 9
          R SAQ QMY
Sbjct: 18 RISAQQQMY 26



>NP_542376
          Length = 1899

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RKSAQFQM 8
            RKSA FQ+
Sbjct: 1532 RKSAAFQI 1539



>NP_004361
          Length = 3063

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RKSAQFQM 8
            RKSA FQ+
Sbjct: 2696 RKSAAFQI 2703



>XP_006715397
          Length = 2972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RKSAQFQM 8
            RKSA FQ+
Sbjct: 2605 RKSAAFQI 2612



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.125    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 293,237
Number of extensions: 262
Number of successful extensions: 7
Number of sequences better than 10000.0: 7
Number of HSP's gapped: 7
Number of HSP's successfully gapped: 7
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)