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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           20     509
NP_036462                                                              20     509
NP_001243397                                                           20     509
XP_005269721                                                           20     509
NP_859526                                                              19     869
NP_002543                                                              19     869
NP_001177810                                                           19     869
NP_001177808                                                           19     869
NP_001177811                                                           19     869
NP_859525                                                              19     869
XP_006712619                                                           19     869
NP_775762                                                              18    1935
NP_000774                                                              17    3301
NP_476498                                                              17    3301
NP_004892                                                              17    3301
NP_001122402                                                           17    3301
NP_653248                                                              17    4312
NP_001036090                                                           17    4312
XP_006714508                                                           17    4312
XP_006714507                                                           17    4312
NP_055917                                                              16    5631
NP_078997                                                              16    5631
XP_006710633                                                           16    5631
XP_006710632                                                           16    5631
XP_006710631                                                           16    5631
XP_006710630                                                           16    5631
XP_006710629                                                           16    5631
XP_006710628                                                           16    5631
XP_006710627                                                           16    5631
XP_006710626                                                           16    5631
NP_683701                                                              16    7355
NP_008816                                                              16    7355
XP_005256014                                                           16    7355
XP_006716052                                                           16    7355
XP_006716051                                                           16    7355
NP_001013682                                                           15    9605
XP_005270918                                                           15    9605

>NP_001243398
          Length = 1781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKPVLMKAF 9
           RKPVL KAF
Sbjct: 893 RKPVLRKAF 901



>NP_036462
          Length = 2073

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            RKPVL KAF
Sbjct: 1185 RKPVLRKAF 1193



>NP_001243397
          Length = 1890

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            RKPVL KAF
Sbjct: 1002 RKPVLRKAF 1010



>XP_005269721
          Length = 2073

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            RKPVL KAF
Sbjct: 1185 RKPVLRKAF 1193



>NP_859526
          Length = 436

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KPVLMKAF 9
           KPV+MKAF
Sbjct: 369 KPVVMKAF 376



>NP_002543
          Length = 436

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KPVLMKAF 9
           KPV+MKAF
Sbjct: 369 KPVVMKAF 376



>NP_001177810
          Length = 352

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KPVLMKAF 9
           KPV+MKAF
Sbjct: 285 KPVVMKAF 292



>NP_001177808
          Length = 436

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KPVLMKAF 9
           KPV+MKAF
Sbjct: 369 KPVVMKAF 376



>NP_001177811
          Length = 362

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KPVLMKAF 9
           KPV+MKAF
Sbjct: 295 KPVVMKAF 302



>NP_859525
          Length = 436

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KPVLMKAF 9
           KPV+MKAF
Sbjct: 369 KPVVMKAF 376



>XP_006712619
          Length = 352

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KPVLMKAF 9
           KPV+MKAF
Sbjct: 285 KPVVMKAF 292



>NP_775762
          Length = 360

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RKPVLMKAF 9
           RKPVL KA+
Sbjct: 206 RKPVLGKAY 214



>NP_000774
          Length = 497

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKPVLMKAF 9
           RK V+M+AF
Sbjct: 136 RKKVIMRAF 144



>NP_476498
          Length = 428

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RKPVLMKAF 9
          RK V+M+AF
Sbjct: 67 RKKVIMRAF 75



>NP_004892
          Length = 616

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RKPVLMK 7
           RKPV+MK
Sbjct: 128 RKPVVMK 134



>NP_001122402
          Length = 608

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RKPVLMK 7
           RKPV+MK
Sbjct: 128 RKPVVMK 134



>NP_653248
          Length = 208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKPVLMKAF 9
           RK V+M+AF
Sbjct: 112 RKEVIMQAF 120



>NP_001036090
          Length = 208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKPVLMKAF 9
           RK V+M+AF
Sbjct: 112 RKEVIMQAF 120



>XP_006714508
          Length = 199

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKPVLMKAF 9
           RK V+M+AF
Sbjct: 129 RKEVIMQAF 137



>XP_006714507
          Length = 225

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKPVLMKAF 9
           RK V+M+AF
Sbjct: 129 RKEVIMQAF 137



>NP_055917
          Length = 1426

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            R+P++ KAF
Sbjct: 1326 RQPLISKAF 1334



>NP_078997
          Length = 3616

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RKPVLM 6
           RKPVLM
Sbjct: 274 RKPVLM 279



>XP_006710633
          Length = 1250

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            R+P++ KAF
Sbjct: 1150 RQPLISKAF 1158



>XP_006710632
          Length = 1389

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            R+P++ KAF
Sbjct: 1289 RQPLISKAF 1297



>XP_006710631
          Length = 1412

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            R+P++ KAF
Sbjct: 1312 RQPLISKAF 1320



>XP_006710630
          Length = 1424

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            R+P++ KAF
Sbjct: 1324 RQPLISKAF 1332



>XP_006710629
          Length = 1425

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            R+P++ KAF
Sbjct: 1325 RQPLISKAF 1333



>XP_006710628
          Length = 1426

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            R+P++ KAF
Sbjct: 1326 RQPLISKAF 1334



>XP_006710627
          Length = 1426

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            R+P++ KAF
Sbjct: 1326 RQPLISKAF 1334



>XP_006710626
          Length = 1426

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKPVLMKAF 9
            R+P++ KAF
Sbjct: 1326 RQPLISKAF 1334



>NP_683701
          Length = 409

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KPVLMKAF 9
           KPV+ K F
Sbjct: 381 KPVIFKVF 388



>NP_008816
          Length = 3703

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RKPVLM 6
           RKP+LM
Sbjct: 278 RKPILM 283



>XP_005256014
          Length = 3702

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RKPVLM 6
           RKP+LM
Sbjct: 278 RKPILM 283



>XP_006716052
          Length = 1409

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  KPVLMKA 8
          KP++MKA
Sbjct: 16 KPLIMKA 22



>XP_006716051
          Length = 1427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  KPVLMKA 8
          KP++MKA
Sbjct: 16 KPLIMKA 22



>NP_001013682
          Length = 626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KPVLMKAF 9
           KPV  KAF
Sbjct: 396 KPVWSKAF 403



>XP_005270918
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KPVLMKAF 9
           KPV  KAF
Sbjct: 396 KPVWSKAF 403



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.144    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 623,327
Number of extensions: 846
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)