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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_570711                                                              20     509
NP_570712                                                              20     509
NP_570714                                                              20     509
NP_570713                                                              20     509
NP_570716                                                              20     509
NP_000235                                                              20     509
NP_570715                                                              20     509
XP_006718620                                                           20     509
XP_005274059                                                           20     509
XP_005274060                                                           20     509
XP_005274058                                                           20     509
NP_060382                                                              16    7355
NP_001158608                                                           16    7355
XP_005263140                                                           16    7355

>NP_570711
          Length = 610

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKMEVVFMV 9
           RKMEV FMV
Sbjct: 232 RKMEVAFMV 240



>NP_570712
          Length = 615

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKMEVVFMV 9
           RKMEV FMV
Sbjct: 237 RKMEVAFMV 245



>NP_570714
          Length = 615

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKMEVVFMV 9
           RKMEV FMV
Sbjct: 237 RKMEVAFMV 245



>NP_570713
          Length = 615

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKMEVVFMV 9
           RKMEV FMV
Sbjct: 237 RKMEVAFMV 245



>NP_570716
          Length = 615

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKMEVVFMV 9
           RKMEV FMV
Sbjct: 237 RKMEVAFMV 245



>NP_000235
          Length = 615

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKMEVVFMV 9
           RKMEV FMV
Sbjct: 237 RKMEVAFMV 245



>NP_570715
          Length = 615

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKMEVVFMV 9
           RKMEV FMV
Sbjct: 237 RKMEVAFMV 245



>XP_006718620
          Length = 610

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKMEVVFMV 9
           RKMEV FMV
Sbjct: 232 RKMEVAFMV 240



>XP_005274059
          Length = 610

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKMEVVFMV 9
           RKMEV FMV
Sbjct: 232 RKMEVAFMV 240



>XP_005274060
          Length = 610

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKMEVVFMV 9
           RKMEV FMV
Sbjct: 232 RKMEVAFMV 240



>XP_005274058
          Length = 610

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKMEVVFMV 9
           RKMEV FMV
Sbjct: 232 RKMEVAFMV 240



>NP_060382
          Length = 1022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RKMEVVF 7
           +KME+VF
Sbjct: 962 QKMEIVF 968



>NP_001158608
          Length = 986

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RKMEVVF 7
           +KME+VF
Sbjct: 926 QKMEIVF 932



>XP_005263140
          Length = 1034

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RKMEVVF 7
           +KME+VF
Sbjct: 974 QKMEIVF 980



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.349    0.147    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 337,656
Number of extensions: 370
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)