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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002040                                                              18    1135
NP_001271132                                                           17    3301
NP_001092095                                                           17    3301
NP_001271131                                                           17    3301
NP_057559                                                              17    3301
NP_001271130                                                           17    3301
NP_001136197                                                           16    5631
NP_112477                                                              16    5631
NP_001243298                                                           16    5631
NP_006632                                                              16    5631
NP_079279                                                              15    9605
NP_001124390                                                           15    9605
NP_001001998                                                           15    9605
XP_006721893                                                           15    9605
XP_006721892                                                           15    9605
XP_006721891                                                           15    9605
XP_006721890                                                           15    9605
XP_005263533                                                           15    9605
XP_005263532                                                           15    9605
NP_002676                                                              15    9605

>NP_002040
          Length = 413

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   KKRLIVSK 9
           KKRLIVSK
Sbjct: 245 KKRLIVSK 252



>NP_001271132
          Length = 248

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   RKKRLIV 7
           RKKRLIV
Sbjct: 184 RKKRLIV 190



>NP_001092095
          Length = 305

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   RKKRLIV 7
           RKKRLIV
Sbjct: 241 RKKRLIV 247



>NP_001271131
          Length = 319

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   RKKRLIV 7
           RKKRLIV
Sbjct: 255 RKKRLIV 261



>NP_057559
          Length = 311

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   RKKRLIV 7
           RKKRLIV
Sbjct: 247 RKKRLIV 253



>NP_001271130
          Length = 325

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   RKKRLIV 7
           RKKRLIV
Sbjct: 261 RKKRLIV 267



>NP_001136197
          Length = 691

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKKRLIVS 8
           R KRL+VS
Sbjct: 602 RSKRLVVS 609



>NP_112477
          Length = 369

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKKRLIVSK 9
           R+KRL+ SK
Sbjct: 158 REKRLLYSK 166



>NP_001243298
          Length = 357

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKKRLIVSK 9
           R+KRL+ SK
Sbjct: 146 REKRLLYSK 154



>NP_006632
          Length = 357

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKKRLIVSK 9
           R+KRL+ SK
Sbjct: 146 REKRLLYSK 154



>NP_079279
          Length = 1910

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RKKRLI 6
           RKKRLI
Sbjct: 250 RKKRLI 255



>NP_001124390
          Length = 891

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RKKRLIVS 8
           +KKRL+++
Sbjct: 105 KKKRLVIN 112



>NP_001001998
          Length = 885

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KKRLIVSK 9
           KKRL +SK
Sbjct: 796 KKRLKISK 803



>XP_006721893
          Length = 713

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RKKRLIVS 8
           +KKRL+++
Sbjct: 105 KKKRLVIN 112



>XP_006721892
          Length = 843

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  RKKRLIVS 8
          +KKRL+++
Sbjct: 57 KKKRLVIN 64



>XP_006721891
          Length = 891

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RKKRLIVS 8
           +KKRL+++
Sbjct: 105 KKKRLVIN 112



>XP_006721890
          Length = 891

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RKKRLIVS 8
           +KKRL+++
Sbjct: 105 KKKRLVIN 112



>XP_005263533
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KKRLIVSK 9
           KKRL +SK
Sbjct: 369 KKRLKISK 376



>XP_005263532
          Length = 914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KKRLIVSK 9
           KKRL +SK
Sbjct: 825 KKRLKISK 832



>NP_002676
          Length = 860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KKRLIVSK 9
           KKRL +SK
Sbjct: 771 KKRLKISK 778



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.137    0.326 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 460,498
Number of extensions: 476
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)