Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              20     509
NP_444254                                                              20     509
NP_444253                                                              20     509
NP_444255                                                              20     509
XP_005247551                                                           20     509
XP_005247550                                                           20     509
XP_006713712                                                           20     509
XP_005247549                                                           20     509
NP_055921                                                              16    5631
XP_006713125                                                           16    5631
XP_006713124                                                           16    5631
NP_005742                                                              15    9605
NP_671714                                                              15    9605
NP_001269516                                                           15    9605
NP_056477                                                              15    9605
NP_001865                                                              15    9605
NP_938079                                                              15    9605
NP_001005502                                                           15    9605
NP_001268451                                                           15    9605
NP_001268449                                                           15    9605
NP_877954                                                              15    9605
NP_001268450                                                           15    9605
NP_115874                                                              15    9605
NP_001438                                                              15    9605
NP_002855                                                              15    9605
NP_003557                                                              15    9605
NP_062832                                                              15    9605
NP_004276                                                              15    9605
XP_005260916                                                           15    9605
XP_006719694                                                           15    9605
XP_005269821                                                           15    9605
XP_006716233                                                           15    9605
XP_006715890                                                           15    9605
XP_005250160                                                           15    9605
XP_005250159                                                           15    9605
XP_006714824                                                           15    9605
XP_006712050                                                           15    9605
XP_005264311                                                           15    9605
XP_006712049                                                           15    9605
XP_006711115                                                           15    9605
XP_005263598                                                           15    9605
XP_005263597                                                           15    9605
XP_005263596                                                           15    9605
NP_001121687                                                           15    9605
NP_443085                                                              15    9605
>NP_444256
          Length = 1794

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKEVTNLIS 9
           RKEVTN+IS
Sbjct: 272 RKEVTNVIS 280
>NP_444254
          Length = 1845

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKEVTNLIS 9
           RKEVTN+IS
Sbjct: 272 RKEVTNVIS 280
>NP_444253
          Length = 1914

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKEVTNLIS 9
           RKEVTN+IS
Sbjct: 272 RKEVTNVIS 280
>NP_444255
          Length = 1863

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKEVTNLIS 9
           RKEVTN+IS
Sbjct: 272 RKEVTNVIS 280
>XP_005247551
          Length = 1855

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKEVTNLIS 9
           RKEVTN+IS
Sbjct: 282 RKEVTNVIS 290
>XP_005247550
          Length = 1856

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKEVTNLIS 9
           RKEVTN+IS
Sbjct: 282 RKEVTNVIS 290
>XP_006713712
          Length = 1923

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKEVTNLIS 9
           RKEVTN+IS
Sbjct: 282 RKEVTNVIS 290
>XP_005247549
          Length = 1924

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKEVTNLIS 9
           RKEVTN+IS
Sbjct: 282 RKEVTNVIS 290
>NP_055921
          Length = 1467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKEVTNLI 8
           RKEV NL+
Sbjct: 900 RKEVENLL 907
>XP_006713125
          Length = 1467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKEVTNLI 8
           RKEV NL+
Sbjct: 900 RKEVENLL 907
>XP_006713124
          Length = 1467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKEVTNLI 8
           RKEV NL+
Sbjct: 900 RKEVENLL 907
>NP_005742
          Length = 3907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    KEVTNL 7
            KE+TNL
Sbjct: 2203 KEITNL 2208
>NP_671714
          Length = 3899

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    KEVTNL 7
            KE+TNL
Sbjct: 2195 KEITNL 2200
>NP_001269516
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RKEVTNLIS 9
           ++E+T L+S
Sbjct: 762 KREITTLVS 770
>NP_056477
          Length = 1749

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KEVTNL 7
           KE+TNL
Sbjct: 281 KEITNL 286
>NP_001865
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EVTNLIS 9
           E+TNLI+
Sbjct: 109 EITNLIN 115
>NP_938079
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EVTNLIS 9
           E+TNLI+
Sbjct: 109 EITNLIN 115
>NP_001005502
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EVTNLIS 9
           E+TNLI+
Sbjct: 109 EITNLIN 115
>NP_001268451
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EVTNLI 8
           EVTNLI
Sbjct: 133 EVTNLI 138
>NP_001268449
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EVTNLI 8
           EVTNLI
Sbjct: 133 EVTNLI 138
>NP_877954
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EVTNLI 8
           EVTNLI
Sbjct: 133 EVTNLI 138
>NP_001268450
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EVTNLI 8
           EVTNLI
Sbjct: 133 EVTNLI 138
>NP_115874
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EVTNLI 8
           EVTNLI
Sbjct: 133 EVTNLI 138
>NP_001438
          Length = 4349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKEVTNLIS 9
            RK+V NL++
Sbjct: 3077 RKDVFNLVA 3085
>NP_002855
          Length = 1482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   EVTNLIS 9
           E+TN++S
Sbjct: 338 EITNIVS 344
>NP_003557
          Length = 1411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    KEVTNL 7
            KE+TNL
Sbjct: 1156 KEITNL 1161
>NP_062832
          Length = 588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EVTNLIS 9
           +VTNL+S
Sbjct: 268 QVTNLVS 274
>NP_004276
          Length = 791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RKEVTNLIS 9
           ++E+T L+S
Sbjct: 751 KREITTLVS 759
>XP_005260916
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EVTNLI 8
           EVTNLI
Sbjct: 133 EVTNLI 138
>XP_006719694
          Length = 1465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    KEVTNL 7
            KE+TNL
Sbjct: 1210 KEITNL 1215
>XP_005269821
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EVTNLI 8
           EVTNLI
Sbjct: 346 EVTNLI 351
>XP_006716233
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EVTNLIS 9
           E TNLIS
Sbjct: 301 EATNLIS 307
>XP_006715890
          Length = 3860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    KEVTNL 7
            KE+TNL
Sbjct: 2156 KEITNL 2161
>XP_005250160
          Length = 3939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    KEVTNL 7
            KE+TNL
Sbjct: 2235 KEITNL 2240
>XP_005250159
          Length = 3952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    KEVTNL 7
            KE+TNL
Sbjct: 2248 KEITNL 2253
>XP_006714824
          Length = 4349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RKEVTNLIS 9
            RK+V NL++
Sbjct: 3077 RKDVFNLVA 3085
>XP_006712050
          Length = 1716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KEVTNL 7
           KE+TNL
Sbjct: 281 KEITNL 286
>XP_005264311
          Length = 1727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KEVTNL 7
           KE+TNL
Sbjct: 281 KEITNL 286
>XP_006712049
          Length = 1728

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KEVTNL 7
           KE+TNL
Sbjct: 260 KEITNL 265
>XP_006711115
          Length = 791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RKEVTNLIS 9
           ++E+T L+S
Sbjct: 751 KREITTLVS 759
>XP_005263598
          Length = 798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RKEVTNLIS 9
           ++E+T L+S
Sbjct: 758 KREITTLVS 766
>XP_005263597
          Length = 800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RKEVTNLIS 9
           ++E+T L+S
Sbjct: 760 KREITTLVS 768
>XP_005263596
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RKEVTNLIS 9
           ++E+T L+S
Sbjct: 762 KREITTLVS 770
>NP_001121687
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   EVTNLI 8
           EVTNLI
Sbjct: 371 EVTNLI 376
>NP_443085
          Length = 626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EVTNLIS 9
           E TNLIS
Sbjct: 301 EATNLIS 307
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.125    0.295 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 420,941
Number of extensions: 775
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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