Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     869
XP_006716142                                                           19     869
XP_005250088                                                           19     869
XP_006716141                                                           19     869
XP_005250085                                                           19     869
XP_006716140                                                           19     869
XP_005250084                                                           19     869
XP_005250083                                                           19     869
XP_005250082                                                           19     869
NP_055901                                                              18    1935
NP_055510                                                              17    2528
XP_005256934                                                           17    2528
XP_006721670                                                           17    2528
NP_075068                                                              17    4312
NP_631981                                                              17    4312
NP_062535                                                              16    5631
XP_005267917                                                           16    5631
NP_057332                                                              16    7355
NP_001264262                                                           16    7355
XP_003959998                                                           16    7355
XP_005266305                                                           16    7355
XP_005266304                                                           16    7355
NP_002537                                                              15    9605
NP_683759                                                              15    9605
NP_003148                                                              15    9605
NP_001277251                                                           15    9605
NP_005500                                                              15    9605
NP_001277250                                                           15    9605
XP_006714623                                                           15    9605
XP_005271970                                                           15    9605
XP_005271969                                                           15    9605
XP_005271967                                                           15    9605
XP_005271966                                                           15    9605
XP_006713373                                                           15    9605
XP_006713055                                                           15    9605
>NP_733751
          Length = 4911

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RKASSQER 8
            RKASSQER
Sbjct: 1683 RKASSQER 1690
>XP_006716142
          Length = 4927

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RKASSQER 8
            RKASSQER
Sbjct: 1700 RKASSQER 1707
>XP_005250088
          Length = 4928

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RKASSQER 8
            RKASSQER
Sbjct: 1700 RKASSQER 1707
>XP_006716141
          Length = 4959

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RKASSQER 8
            RKASSQER
Sbjct: 1700 RKASSQER 1707
>XP_005250085
          Length = 4979

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RKASSQER 8
            RKASSQER
Sbjct: 1700 RKASSQER 1707
>XP_006716140
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RKASSQER 8
            RKASSQER
Sbjct: 1700 RKASSQER 1707
>XP_005250084
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RKASSQER 8
            RKASSQER
Sbjct: 1700 RKASSQER 1707
>XP_005250083
          Length = 4982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RKASSQER 8
            RKASSQER
Sbjct: 1699 RKASSQER 1706
>XP_005250082
          Length = 4983

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    RKASSQER 8
            RKASSQER
Sbjct: 1700 RKASSQER 1707
>NP_055901
          Length = 711

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RKASSQER 8
           RKA+SQER
Sbjct: 113 RKAASQER 120
>NP_055510
          Length = 1427

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    RKASSQERT 9
            +KA+SQE+T
Sbjct: 1061 KKANSQEKT 1069
>XP_005256934
          Length = 1434

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    RKASSQERT 9
            +KA+SQE+T
Sbjct: 1061 KKANSQEKT 1069
>XP_006721670
          Length = 1471

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    RKASSQERT 9
            +KA+SQE+T
Sbjct: 1061 KKANSQEKT 1069
>NP_075068
          Length = 1146

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  RKASSQERT 9
          +KASS++RT
Sbjct: 32 KKASSRKRT 40
>NP_631981
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  RKASSQERT 9
          +KASS++RT
Sbjct: 32 KKASSRKRT 40
>NP_062535
          Length = 2146

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RKASSQER 8
            R+A SQER
Sbjct: 1115 RRAGSQER 1122
>XP_005267917
          Length = 2061

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RKASSQER 8
            R+A SQER
Sbjct: 1115 RRAGSQER 1122
>NP_057332
          Length = 1901

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    KASSQER 8
            KASS+ER
Sbjct: 1883 KASSEER 1889
>NP_001264262
          Length = 917

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RKASSQERT 9
           +KA +QE+T
Sbjct: 795 KKADNQEKT 803
>XP_003959998
          Length = 639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RKASSQERT 9
           +KA +QE+T
Sbjct: 517 KKADNQEKT 525
>XP_005266305
          Length = 1856

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    KASSQER 8
            KASS+ER
Sbjct: 1838 KASSEER 1844
>XP_005266304
          Length = 1873

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    KASSQER 8
            KASS+ER
Sbjct: 1855 KASSEER 1861
>NP_002537
          Length = 401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKASSQERT 9
           R+ SSQE+T
Sbjct: 242 RQHSSQEQT 250
>NP_683759
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KASSQERT 9
           KAS+ ERT
Sbjct: 435 KASAPERT 442
>NP_003148
          Length = 841

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RKASSQER 8
          R ASS+ER
Sbjct: 40 RNASSRER 47
>NP_001277251
          Length = 2854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKASSQER 8
           R ASS+ER
Sbjct: 125 RNASSKER 132
>NP_005500
          Length = 3027

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKASSQER 8
           R ASS+ER
Sbjct: 298 RNASSKER 305
>NP_001277250
          Length = 3048

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKASSQER 8
           R ASS+ER
Sbjct: 298 RNASSKER 305
>XP_006714623
          Length = 1625

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKASSQER 8
           R ASS+ER
Sbjct: 298 RNASSKER 305
>XP_005271970
          Length = 2931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKASSQER 8
           R ASS+ER
Sbjct: 298 RNASSKER 305
>XP_005271969
          Length = 3020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKASSQER 8
           R ASS+ER
Sbjct: 232 RNASSKER 239
>XP_005271967
          Length = 3065

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKASSQER 8
           R ASS+ER
Sbjct: 298 RNASSKER 305
>XP_005271966
          Length = 3086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKASSQER 8
           R ASS+ER
Sbjct: 298 RNASSKER 305
>XP_006713373
          Length = 795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RKASSQER 8
          R ASS+ER
Sbjct: 40 RNASSRER 47
>XP_006713055
          Length = 195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RKASSQERT 9
           +KA+S +RT
Sbjct: 185 KKATSSQRT 193
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.290   0.0975    0.216 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 370,734
Number of extensions: 600
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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