Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              19     869
NP_001013650                                                           17    2528
NP_006421                                                              17    3301
NP_001243650                                                           17    3301
NP_060206                                                              16    5631
XP_005255481                                                           16    5631
XP_005255479                                                           16    5631
NP_001092879                                                           16    7355
NP_006315                                                              16    7355
XP_005273924                                                           16    7355
XP_006718550                                                           16    7355
XP_006718549                                                           16    7355
XP_005276064                                                           16    7355
NP_055453                                                              15    9605
NP_004870                                                              15    9605
NP_001277064                                                           15    9605
NP_113609                                                              15    9605
XP_006725014                                                           15    9605
XP_006725013                                                           15    9605
XP_006725012                                                           15    9605
XP_006722192                                                           15    9605
XP_006711118                                                           15    9605
XP_006711117                                                           15    9605
>NP_004351
          Length = 882

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RKAQSVEAG 9
           RKAQ VEAG
Sbjct: 696 RKAQPVEAG 704
>NP_001013650
          Length = 463

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  RKAQSVEAG 9
          RKA +VEAG
Sbjct: 11 RKAPAVEAG 19
>NP_006421
          Length = 539

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KAQSVEAG 9
           KAQ +EAG
Sbjct: 96  KAQDIEAG 103
>NP_001243650
          Length = 509

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KAQSVEAG 9
          KAQ +EAG
Sbjct: 66 KAQDIEAG 73
>NP_060206
          Length = 353

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKAQSVEAG 9
           R+ QSVE+G
Sbjct: 115 RRFQSVESG 123
>XP_005255481
          Length = 439

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKAQSVEAG 9
           R+ QSVE+G
Sbjct: 201 RRFQSVESG 209
>XP_005255479
          Length = 439

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RKAQSVEAG 9
           R+ QSVE+G
Sbjct: 201 RRFQSVESG 209
>NP_001092879
          Length = 1523

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KAQSVEAG 9
           KAQ  EAG
Sbjct: 973 KAQEAEAG 980
>NP_006315
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RKAQSVEA 8
          RKAQS+ A
Sbjct: 53 RKAQSIPA 60
>XP_005273924
          Length = 993

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KAQSVEAG 9
           KAQ  EAG
Sbjct: 443 KAQEAEAG 450
>XP_006718550
          Length = 1063

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KAQSVEAG 9
           KAQ  EAG
Sbjct: 985 KAQEAEAG 992
>XP_006718549
          Length = 1535

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KAQSVEAG 9
           KAQ  EAG
Sbjct: 985 KAQEAEAG 992
>XP_005276064
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AQSVEAG 9
           AQS+EAG
Sbjct: 291 AQSLEAG 297
>NP_055453
          Length = 1416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RKAQSVEAG 9
           +KA SVE G
Sbjct: 591 KKAASVEEG 599
>NP_004870
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  RKAQSVE 7
          R+AQS+E
Sbjct: 52 RRAQSIE 58
>NP_001277064
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  RKAQSVE 7
          R+AQS+E
Sbjct: 38 RRAQSIE 44
>NP_113609
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QSVEAG 9
           QSVEAG
Sbjct: 567 QSVEAG 572
>XP_006725014
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RKAQSVEAG 9
           +KA SVE G
Sbjct: 564 KKAASVEEG 572
>XP_006725013
          Length = 1389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RKAQSVEAG 9
           +KA SVE G
Sbjct: 564 KKAASVEEG 572
>XP_006725012
          Length = 1388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RKAQSVEAG 9
           +KA SVE G
Sbjct: 563 KKAASVEEG 571
>XP_006722192
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QSVEAG 9
           QSVEAG
Sbjct: 404 QSVEAG 409
>XP_006711118
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RKAQSVEAG 9
           +KA SVE G
Sbjct: 564 KKAASVEEG 572
>XP_006711117
          Length = 1413

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RKAQSVEAG 9
           +KA SVE G
Sbjct: 588 KKAASVEEG 596
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.298    0.111    0.265 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 268,204
Number of extensions: 321
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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