Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           20     390
NP_001257437                                                           20     390
NP_006281                                                              20     390
XP_005267176                                                           20     390
NP_057692                                                              17    3301
NP_258428                                                              16    5631
NP_004288                                                              15    9605
NP_001193940                                                           15    9605
NP_835471                                                              15    9605
NP_001269221                                                           15    9605
NP_009119                                                              15    9605
NP_001269220                                                           15    9605
NP_671693                                                              15    9605
NP_001833                                                              15    9605
NP_001269222                                                           15    9605
XP_006716783                                                           15    9605
XP_005251420                                                           15    9605
XP_005251419                                                           15    9605
XP_006713692                                                           15    9605
XP_006713691                                                           15    9605
XP_006713690                                                           15    9605
XP_006713689                                                           15    9605
XP_006713688                                                           15    9605
XP_006713687                                                           15    9605
>NP_001257436
          Length = 790

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKALFSTIK 9
           RKALFST+K
Sbjct: 123 RKALFSTLK 131
>NP_001257437
          Length = 790

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKALFSTIK 9
           RKALFST+K
Sbjct: 123 RKALFSTLK 131
>NP_006281
          Length = 790

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKALFSTIK 9
           RKALFST+K
Sbjct: 123 RKALFSTLK 131
>XP_005267176
          Length = 790

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RKALFSTIK 9
           RKALFST+K
Sbjct: 123 RKALFSTLK 131
>NP_057692
          Length = 453

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KALFSTIK 9
           KALF+T+K
Sbjct: 109 KALFNTLK 116
>NP_258428
          Length = 779

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RKALFSTI 8
           RKALF T+
Sbjct: 423 RKALFGTM 430
>NP_004288
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KALFSTI 8
           KALF TI
Sbjct: 248 KALFKTI 254
>NP_001193940
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFSTIK 9
           LFSTIK
Sbjct: 171 LFSTIK 176
>NP_835471
          Length = 215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  KALFST 7
          KALFST
Sbjct: 81 KALFST 86
>NP_001269221
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RKALFSTI 8
           R+ALFS++
Sbjct: 211 RRALFSSL 218
>NP_009119
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RKALFSTI 8
           R+ALFS++
Sbjct: 211 RRALFSSL 218
>NP_001269220
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RKALFSTI 8
           R+ALFS++
Sbjct: 228 RRALFSSL 235
>NP_671693
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFSTIK 9
           LFSTIK
Sbjct: 171 LFSTIK 176
>NP_001833
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFSTIK 9
           LFSTIK
Sbjct: 171 LFSTIK 176
>NP_001269222
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RKALFSTI 8
           R+ALFS++
Sbjct: 123 RRALFSSL 130
>XP_006716783
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFSTIK 9
           LFSTIK
Sbjct: 171 LFSTIK 176
>XP_005251420
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFSTIK 9
           LFSTIK
Sbjct: 171 LFSTIK 176
>XP_005251419
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LFSTIK 9
           LFSTIK
Sbjct: 171 LFSTIK 176
>XP_006713692
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KALFST 7
           KALFST
Sbjct: 118 KALFST 123
>XP_006713691
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KALFST 7
           KALFST
Sbjct: 118 KALFST 123
>XP_006713690
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KALFST 7
           KALFST
Sbjct: 212 KALFST 217
>XP_006713689
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KALFST 7
           KALFST
Sbjct: 212 KALFST 217
>XP_006713688
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KALFST 7
           KALFST
Sbjct: 212 KALFST 217
>XP_006713687
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   KALFST 7
           KALFST
Sbjct: 212 KALFST 217
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.134    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 338,145
Number of extensions: 328
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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