Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_689966                                                              18    1482
XP_005249005                                                           18    1482
XP_005249004                                                           18    1482
XP_005249003                                                           18    1482
XP_005249000                                                           18    1482
NP_705834                                                              17    4312
NP_001269939                                                           16    7355
NP_001269938                                                           16    7355
NP_116575                                                              16    7355
NP_057518                                                              16    7355
NP_570969                                                              16    7355
NP_055918                                                              16    7355
NP_055455                                                              16    7355
NP_714544                                                              16    7355
XP_006723946                                                           16    7355
XP_006720470                                                           16    7355
XP_005247035                                                           16    7355
XP_006712939                                                           16    7355
XP_006712938                                                           16    7355
XP_006712937                                                           16    7355
XP_006712936                                                           16    7355
XP_005247034                                                           16    7355
XP_006710049                                                           16    7355
XP_005276017                                                           16    7355
NP_001175                                                              15    9605
NP_001191150                                                           15    9605
NP_443128                                                              15    9605
NP_005910                                                              15    9605
XP_005276647                                                           15    9605
XP_005276648                                                           15    9605
XP_006726767                                                           15    9605
>NP_689966
          Length = 993

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RISSFLRP 8
           ++SSFLRP
Sbjct: 984 KVSSFLRP 991
>XP_005249005
          Length = 531

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RISSFLRP 8
           ++SSFLRP
Sbjct: 522 KVSSFLRP 529
>XP_005249004
          Length = 955

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RISSFLRP 8
           ++SSFLRP
Sbjct: 946 KVSSFLRP 953
>XP_005249003
          Length = 987

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RISSFLRP 8
           ++SSFLRP
Sbjct: 978 KVSSFLRP 985
>XP_005249000
          Length = 1009

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    RISSFLRP 8
            ++SSFLRP
Sbjct: 1000 KVSSFLRP 1007
>NP_705834
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RISSFLR 7
           R+SSFLR
Sbjct: 525 RVSSFLR 531
>NP_001269939
          Length = 996

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RISSFLR 7
           +ISSFLR
Sbjct: 957 KISSFLR 963
>NP_001269938
          Length = 1300

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RISSFLR 7
            +ISSFLR
Sbjct: 1180 KISSFLR 1186
>NP_116575
          Length = 1243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RISSFLR 7
            +ISSFLR
Sbjct: 1204 KISSFLR 1210
>NP_057518
          Length = 1219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RISSFLR 7
            +ISSFLR
Sbjct: 1180 KISSFLR 1186
>NP_570969
          Length = 605

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RISSFLR 7
           +ISSFLR
Sbjct: 533 KISSFLR 539
>NP_055918
          Length = 1925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISSFLRPS 9
           +S+FL PS
Sbjct: 280 VSAFLHPS 287
>NP_055455
          Length = 1199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSFLRPS 9
           SSFL PS
Sbjct: 361 SSFLNPS 367
>NP_714544
          Length = 1775

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ISSFLRPS 9
            +SSFLR S
Sbjct: 1572 VSSFLRQS 1579
>XP_006723946
          Length = 1414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    ISSFLRP 8
            ++SFLRP
Sbjct: 1386 LASFLRP 1392
>XP_006720470
          Length = 1017

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ISSFLRPS 9
           +SSFLR S
Sbjct: 814 VSSFLRQS 821
>XP_005247035
          Length = 971

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSFLRPS 9
           SSFL PS
Sbjct: 361 SSFLNPS 367
>XP_006712939
          Length = 972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSFLRPS 9
           SSFL PS
Sbjct: 361 SSFLNPS 367
>XP_006712938
          Length = 1138

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSFLRPS 9
           SSFL PS
Sbjct: 361 SSFLNPS 367
>XP_006712937
          Length = 1199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSFLRPS 9
           SSFL PS
Sbjct: 361 SSFLNPS 367
>XP_006712936
          Length = 1199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSFLRPS 9
           SSFL PS
Sbjct: 361 SSFLNPS 367
>XP_005247034
          Length = 1199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSFLRPS 9
           SSFL PS
Sbjct: 361 SSFLNPS 367
>XP_006710049
          Length = 293

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  ISSFLRPS 9
          +SSFLR S
Sbjct: 90 VSSFLRQS 97
>XP_005276017
          Length = 610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ISSFLRP 8
           ++SFLRP
Sbjct: 571 LASFLRP 577
>NP_001175
          Length = 2644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RISSFLRPS 9
           R+SS L PS
Sbjct: 433 RLSSSLNPS 441
>NP_001191150
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ISSFLRP 8
           I SFLRP
Sbjct: 145 ILSFLRP 151
>NP_443128
          Length = 3487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    RISSFLR 7
            +ISSFLR
Sbjct: 2081 QISSFLR 2087
>NP_005910
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SSFLRPS 9
           ++FLRPS
Sbjct: 294 ATFLRPS 300
>XP_005276647
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ISSFLRP 8
           +SS+LRP
Sbjct: 119 VSSWLRP 125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ISSFLRP 8
           +SS+LRP
Sbjct: 252 VSSWLRP 258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ISSFLRP 8
           +SS+LRP
Sbjct: 404 VSSWLRP 410
>XP_005276648
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ISSFLRP 8
           +SS+LRP
Sbjct: 119 VSSWLRP 125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ISSFLRP 8
           +SS+LRP
Sbjct: 252 VSSWLRP 258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ISSFLRP 8
           +SS+LRP
Sbjct: 404 VSSWLRP 410
>XP_006726767
          Length = 615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ISSFLRP 8
           +SS+LRP
Sbjct: 119 VSSWLRP 125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ISSFLRP 8
           +SS+LRP
Sbjct: 252 VSSWLRP 258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ISSFLRP 8
           +SS+LRP
Sbjct: 404 VSSWLRP 410
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.137    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 563,770
Number of extensions: 1055
Number of successful extensions: 37
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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