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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1135
NP_001075109                                                           18    1135
NP_005449                                                              16    5631
XP_005252373                                                           16    5631
XP_006711056                                                           16    7355
NP_008914                                                              16    7355
NP_055873                                                              15    9605
NP_001009814                                                           15    9605
NP_001275915                                                           15    9605
NP_001275914                                                           15    9605
NP_009099                                                              15    9605
XP_005256997                                                           15    9605
XP_005256995                                                           15    9605
XP_006719855                                                           15    9605
XP_006719854                                                           15    9605
XP_006719853                                                           15    9605

>NP_008835
          Length = 4128

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    RIIANALNS 9
            RIIANAL+S
Sbjct: 3335 RIIANALSS 3343



>NP_001075109
          Length = 4097

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    RIIANALNS 9
            RIIANAL+S
Sbjct: 3335 RIIANALSS 3343



>NP_005449
          Length = 941

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RIIANALN 8
           RII NA+N
Sbjct: 397 RIILNAMN 404



>XP_005252373
          Length = 683

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RIIANALN 8
           RII NA+N
Sbjct: 139 RIILNAMN 146



>XP_006711056
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IIANALN 8
           IIANA+N
Sbjct: 357 IIANAVN 363



>NP_008914
          Length = 390

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IIANALN 8
           IIANA+N
Sbjct: 358 IIANAVN 364



>NP_055873
          Length = 1905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RIIANALN 8
           RI+AN+ N
Sbjct: 876 RIVANSAN 883



>NP_001009814
          Length = 1039

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RIIANALN 8
           RI+AN+ N
Sbjct: 876 RIVANSAN 883



>NP_001275915
          Length = 1616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    ANALNS 9
            ANALNS
Sbjct: 1261 ANALNS 1266



>NP_001275914
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    ANALNS 9
            ANALNS
Sbjct: 1266 ANALNS 1271



>NP_009099
          Length = 1581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    ANALNS 9
            ANALNS
Sbjct: 1226 ANALNS 1231



>XP_005256997
          Length = 1560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    ANALNS 9
            ANALNS
Sbjct: 1205 ANALNS 1210



>XP_005256995
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    ANALNS 9
            ANALNS
Sbjct: 1266 ANALNS 1271



>XP_006719855
          Length = 1046

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RIIANALN 8
           RI+AN+ N
Sbjct: 876 RIVANSAN 883



>XP_006719854
          Length = 1550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RIIANALN 8
           RI+AN+ N
Sbjct: 521 RIVANSAN 528



>XP_006719853
          Length = 1809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RIIANALN 8
           RI+AN+ N
Sbjct: 780 RIVANSAN 787



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.129    0.313 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 249,337
Number of extensions: 314
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)