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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064450                                                              19     869
XP_005273257                                                           19     869
NP_001004752                                                           16    5631
NP_945327                                                              16    7355
NP_055573                                                              15    9605
NP_001017967                                                           15    9605
NP_001122312                                                           15    9605
NP_001122311                                                           15    9605
NP_000711                                                              15    9605
NP_000709                                                              15    9605
NP_001230741                                                           15    9605
XP_005271603                                                           15    9605
XP_005271602                                                           15    9605
XP_005265505                                                           15    9605
XP_006713388                                                           15    9605
NP_149420                                                              15    9605

>NP_064450
          Length = 1722

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RIIAMGVLL 9
           RIIAMG+LL
Sbjct: 564 RIIAMGLLL 572



>XP_005273257
          Length = 1726

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RIIAMGVLL 9
           RIIAMG+LL
Sbjct: 564 RIIAMGLLL 572



>NP_001004752
          Length = 312

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIIAMGVLL 9
           RII MG+L+
Sbjct: 142 RIIQMGLLM 150



>NP_945327
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IIAMGVL 8
           ++AMGVL
Sbjct: 431 VVAMGVL 437



>NP_055573
          Length = 992

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RIIAMGVLL 9
          +++A+GVLL
Sbjct: 28 KLMAVGVLL 36



>NP_001017967
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IIAMGVLL 9
           ++ MGVLL
Sbjct: 279 VLTMGVLL 286



>NP_001122312
          Length = 2161

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIIAMGVLL 9
           +IIA G+LL
Sbjct: 179 KIIAYGLLL 187



>NP_001122311
          Length = 2137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIIAMGVLL 9
           +IIA G+LL
Sbjct: 179 KIIAYGLLL 187



>NP_000711
          Length = 2181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIIAMGVLL 9
           +IIA G+LL
Sbjct: 179 KIIAYGLLL 187



>NP_000709
          Length = 2339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RIIAMGVL 8
            +IIA GVL
Sbjct: 1521 KIIAFGVL 1528



>NP_001230741
          Length = 2237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    RIIAMGVL 8
            +IIA GVL
Sbjct: 1521 KIIAFGVL 1528



>XP_005271603
          Length = 907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RIIAMGVLL 9
          +++A+GVLL
Sbjct: 28 KLMAVGVLL 36



>XP_005271602
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RIIAMGVLL 9
          +++A+GVLL
Sbjct: 28 KLMAVGVLL 36



>XP_005265505
          Length = 2146

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIIAMGVLL 9
           +IIA G+LL
Sbjct: 179 KIIAYGLLL 187



>XP_006713388
          Length = 2194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIIAMGVLL 9
           +IIA G+LL
Sbjct: 179 KIIAYGLLL 187



>NP_149420
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RIIAMGV 7
           R+IA+GV
Sbjct: 138 RVIALGV 144



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.350    0.164    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 334,066
Number of extensions: 481
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)