Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              20     390
XP_005251512                                                           20     390
NP_001008392                                                           18    1135
NP_001103159                                                           17    3301
NP_066405                                                              17    3301
XP_005272388                                                           17    3301
XP_005272387                                                           17    3301
XP_005272385                                                           17    3301
XP_005272384                                                           17    3301
XP_006716676                                                           17    3301
XP_006716675                                                           17    3301
NP_071504                                                              17    4312
NP_000911                                                              17    4312
NP_001035806                                                           17    4312
NP_004161                                                              17    4312
NP_005036                                                              17    4312
NP_774959                                                              17    4312
XP_006718641                                                           17    4312
XP_005274089                                                           17    4312
XP_005274088                                                           17    4312
XP_006718640                                                           17    4312
NP_057576                                                              16    5631
NP_001171272                                                           16    5631
NP_001171273                                                           16    5631
NP_065944                                                              16    5631
NP_055758                                                              16    5631
XP_005256675                                                           16    5631
XP_005250893                                                           16    5631
XP_005250894                                                           16    5631
NP_001238892                                                           16    7355
NP_001238891                                                           16    7355
NP_056410                                                              16    7355
XP_006725061                                                           16    7355
XP_005245618                                                           16    7355
NP_003657                                                              16    7355
NP_001005198                                                           15    9605
NP_001265354                                                           15    9605
NP_003622                                                              15    9605
NP_849157                                                              15    9605
NP_009153                                                              15    9605
NP_001017926                                                           15    9605
NP_443124                                                              15    9605
NP_859062                                                              15    9605
XP_006719324                                                           15    9605
XP_006714159                                                           15    9605
XP_006714158                                                           15    9605
XP_006714157                                                           15    9605
>NP_004963
          Length = 1132

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RIAKENNQT 9
           RIAKEN+QT
Sbjct: 188 RIAKENDQT 196
>XP_005251512
          Length = 1132

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RIAKENNQT 9
           RIAKEN+QT
Sbjct: 188 RIAKENDQT 196
>NP_001008392
          Length = 1079

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           ++ KENNQT
Sbjct: 306 KVLKENNQT 314
>NP_001103159
          Length = 555

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           RI +ENN+T
Sbjct: 148 RIDQENNET 156
>NP_066405
          Length = 560

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           RI +ENN+T
Sbjct: 153 RIDQENNET 161
>XP_005272388
          Length = 534

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           RI +ENN+T
Sbjct: 127 RIDQENNET 135
>XP_005272387
          Length = 539

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           RI +ENN+T
Sbjct: 132 RIDQENNET 140
>XP_005272385
          Length = 555

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           RI +ENN+T
Sbjct: 148 RIDQENNET 156
>XP_005272384
          Length = 555

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           RI +ENN+T
Sbjct: 148 RIDQENNET 156
>XP_006716676
          Length = 560

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           RI +ENN+T
Sbjct: 153 RIDQENNET 161
>XP_006716675
          Length = 560

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           RI +ENN+T
Sbjct: 153 RIDQENNET 161
>NP_071504
          Length = 1178

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RIAKENN 7
           ++AKENN
Sbjct: 104 KVAKENN 110
>NP_000911
          Length = 1178

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RIAKENN 7
           ++AKENN
Sbjct: 104 KVAKENN 110
>NP_001035806
          Length = 1178

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RIAKENN 7
           ++AKENN
Sbjct: 104 KVAKENN 110
>NP_004161
          Length = 524

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RIAKENNQ 8
           R A+ENNQ
Sbjct: 342 RCAEENNQ 349
>NP_005036
          Length = 3460

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    RIAKENNQT 9
            RI  ENN T
Sbjct: 2955 RIGSENNMT 2963
>NP_774959
          Length = 3458

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    RIAKENNQT 9
            RI  ENN T
Sbjct: 2955 RIGSENNMT 2963
>XP_006718641
          Length = 1178

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RIAKENN 7
           ++AKENN
Sbjct: 104 KVAKENN 110
>XP_005274089
          Length = 1178

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RIAKENN 7
           ++AKENN
Sbjct: 104 KVAKENN 110
>XP_005274088
          Length = 1178

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RIAKENN 7
           ++AKENN
Sbjct: 104 KVAKENN 110
>XP_006718640
          Length = 1178

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RIAKENN 7
           ++AKENN
Sbjct: 104 KVAKENN 110
>NP_057576
          Length = 186

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RIAKENNQ 8
           +IAKEN Q
Sbjct: 110 QIAKENQQ 117
>NP_001171272
          Length = 146

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RIAKENNQ 8
           +IAKEN Q
Sbjct: 110 QIAKENQQ 117
>NP_001171273
          Length = 165

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RIAKENNQ 8
           +IAKEN Q
Sbjct: 110 QIAKENQQ 117
>NP_065944
          Length = 1701

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RIAKENNQT 9
            ++AK N+QT
Sbjct: 1132 QVAKSNSQT 1140
>NP_055758
          Length = 837

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RIAKENNQT 9
           ++ K NNQT
Sbjct: 142 KLIKRNNQT 150
>XP_005256675
          Length = 118

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RIAKENNQ 8
           +IAKEN Q
Sbjct: 110 QIAKENQQ 117
>XP_005250893
          Length = 837

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RIAKENNQT 9
           ++ K NNQT
Sbjct: 142 KLIKRNNQT 150
>XP_005250894
          Length = 837

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RIAKENNQT 9
           ++ K NNQT
Sbjct: 142 KLIKRNNQT 150
>NP_001238892
          Length = 791

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RIAKENNQ 8
           +I+K NNQ
Sbjct: 260 KISKSNNQ 267
>NP_001238891
          Length = 871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RIAKENNQ 8
           +I+K NNQ
Sbjct: 260 KISKSNNQ 267
>NP_056410
          Length = 871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RIAKENNQ 8
           +I+K NNQ
Sbjct: 260 KISKSNNQ 267
>XP_006725061
          Length = 904

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RIAKENNQ 8
           +I+K NNQ
Sbjct: 260 KISKSNNQ 267
>XP_005245618
          Length = 267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RIAKENNQ 8
           R+A+E NQ
Sbjct: 179 RLAREKNQ 186
>NP_003657
          Length = 400

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RIAKENNQ 8
           R+A+E NQ
Sbjct: 179 RLAREKNQ 186
>NP_001005198
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  IAKENNQT 9
          + KENN T
Sbjct: 11 LQKENNNT 18
>NP_001265354
          Length = 1774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RIAKENN 7
           R+AKE N
Sbjct: 936 RVAKEQN 942
>NP_003622
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   AKENNQT 9
           +KENN T
Sbjct: 98  SKENNNT 104
>NP_849157
          Length = 1077

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           +++KE+N T
Sbjct: 742 KVSKEHNHT 750
>NP_009153
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   IAKENNQT 9
           + K NNQT
Sbjct: 135 MVKRNNQT 142
>NP_001017926
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   IAKENNQT 9
           + K NNQT
Sbjct: 135 MVKRNNQT 142
>NP_443124
          Length = 2459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    RIAKENN 7
            R+AKE N
Sbjct: 1621 RVAKEQN 1627
>NP_859062
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IAKENNQT 9
           + KE+NQT
Sbjct: 104 LEKEDNQT 111
>XP_006719324
          Length = 210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IAKENNQT 9
           + KE+NQT
Sbjct: 104 LEKEDNQT 111
>XP_006714159
          Length = 1077

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           +++KE+N T
Sbjct: 742 KVSKEHNHT 750
>XP_006714158
          Length = 1077

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           +++KE+N T
Sbjct: 742 KVSKEHNHT 750
>XP_006714157
          Length = 1077

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RIAKENNQT 9
           +++KE+N T
Sbjct: 742 KVSKEHNHT 750
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.112    0.275 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 615,986
Number of extensions: 866
Number of successful extensions: 47
Number of sequences better than 10000.0: 47
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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