Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              20     390
NP_996778                                                              17    2528
NP_001180240                                                           17    2528
XP_006715838                                                           17    2528
XP_005246606                                                           17    2528
XP_005246605                                                           17    2528
NP_001180241                                                           17    2528
NP_079004                                                              17    2528
NP_001180242                                                           17    2528
NP_056247                                                              17    3301
NP_001123203                                                           17    3301
NP_001123199                                                           17    3301
NP_001123200                                                           17    3301
NP_001123201                                                           17    3301
NP_071928                                                              17    4312
NP_001003845                                                           17    4312
XP_006714549                                                           17    4312
XP_006714548                                                           17    4312
XP_005248392                                                           17    4312
XP_005248393                                                           17    4312
XP_005248391                                                           17    4312
XP_005248390                                                           17    4312
XP_005246599                                                           17    4312
NP_002262                                                              16    5631
NP_277045                                                              16    5631
XP_005254110                                                           16    5631
XP_005254109                                                           16    5631
XP_005254106                                                           16    5631
NP_001188467                                                           16    7355
NP_001138820                                                           16    7355
NP_004033                                                              16    7355
NP_079049                                                              16    7355
NP_001188468                                                           16    7355
XP_006721155                                                           16    7355
NP_073741                                                              15    9605
NP_001120926                                                           15    9605
NP_001120925                                                           15    9605
NP_006179                                                              15    9605
NP_004394                                                              15    9605
NP_001091091                                                           15    9605
>NP_004251
          Length = 1208

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RIAALLQTC 9
           RIAALL+TC
Sbjct: 715 RIAALLRTC 723
>NP_996778
          Length = 121

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   IAALLQTC 9
           IAALL TC
Sbjct: 98  IAALLATC 105
>NP_001180240
          Length = 471

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RIAALLQTC 9
          R+AAL +TC
Sbjct: 14 RVAALRRTC 22
>XP_006715838
          Length = 471

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RIAALLQTC 9
          R+AAL +TC
Sbjct: 14 RVAALRRTC 22
>XP_005246606
          Length = 121

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   IAALLQTC 9
           IAALL TC
Sbjct: 98  IAALLATC 105
>XP_005246605
          Length = 121

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   IAALLQTC 9
           IAALL TC
Sbjct: 98  IAALLATC 105
>NP_001180241
          Length = 397

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RIAALLQTC 9
          R+AAL +TC
Sbjct: 14 RVAALRRTC 22
>NP_079004
          Length = 434

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RIAALLQTC 9
          R+AAL +TC
Sbjct: 14 RVAALRRTC 22
>NP_001180242
          Length = 445

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RIAALLQTC 9
          R+AAL +TC
Sbjct: 14 RVAALRRTC 22
>NP_056247
          Length = 1191

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RIAALLQTC 9
           R+ ALLQ C
Sbjct: 336 RLEALLQNC 344
>NP_001123203
          Length = 1110

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RIAALLQTC 9
           R+ ALLQ C
Sbjct: 255 RLEALLQNC 263
>NP_001123199
          Length = 1191

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RIAALLQTC 9
           R+ ALLQ C
Sbjct: 336 RLEALLQNC 344
>NP_001123200
          Length = 1191

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RIAALLQTC 9
           R+ ALLQ C
Sbjct: 336 RLEALLQNC 344
>NP_001123201
          Length = 1191

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RIAALLQTC 9
           R+ ALLQ C
Sbjct: 336 RLEALLQNC 344
>NP_071928
          Length = 215

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ALLQTC 9
          ALLQTC
Sbjct: 10 ALLQTC 15
>NP_001003845
          Length = 398

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RIAALLQT 8
           +IAALLQT
Sbjct: 253 QIAALLQT 260
>XP_006714549
          Length = 215

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ALLQTC 9
          ALLQTC
Sbjct: 10 ALLQTC 15
>XP_006714548
          Length = 215

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ALLQTC 9
          ALLQTC
Sbjct: 10 ALLQTC 15
>XP_005248392
          Length = 215

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ALLQTC 9
          ALLQTC
Sbjct: 10 ALLQTC 15
>XP_005248393
          Length = 215

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ALLQTC 9
          ALLQTC
Sbjct: 10 ALLQTC 15
>XP_005248391
          Length = 215

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ALLQTC 9
          ALLQTC
Sbjct: 10 ALLQTC 15
>XP_005248390
          Length = 215

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ALLQTC 9
          ALLQTC
Sbjct: 10 ALLQTC 15
>XP_005246599
          Length = 442

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RIAALLQT 8
           +IAALLQT
Sbjct: 297 QIAALLQT 304
>NP_002262
          Length = 1115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   IAALLQT 8
           IAALLQT
Sbjct: 461 IAALLQT 467
>NP_277045
          Length = 1401

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RIAALLQTC 9
           R  ALL+TC
Sbjct: 363 RTLALLRTC 371
>XP_005254110
          Length = 1115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   IAALLQT 8
           IAALLQT
Sbjct: 461 IAALLQT 467
>XP_005254109
          Length = 1115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   IAALLQT 8
           IAALLQT
Sbjct: 461 IAALLQT 467
>XP_005254106
          Length = 1115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   IAALLQT 8
           IAALLQT
Sbjct: 461 IAALLQT 467
>NP_001188467
          Length = 590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  IAALLQTC 9
          + ALL TC
Sbjct: 13 VCALLNTC 20
>NP_001138820
          Length = 454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RIAALLQT 8
           ++AA+LQT
Sbjct: 178 KVAAILQT 185
>NP_004033
          Length = 590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  IAALLQTC 9
          + ALL TC
Sbjct: 13 VCALLNTC 20
>NP_079049
          Length = 416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RIAALLQT 8
           ++AA+LQT
Sbjct: 140 KVAAILQT 147
>NP_001188468
          Length = 590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  IAALLQTC 9
          + ALL TC
Sbjct: 13 VCALLNTC 20
>XP_006721155
          Length = 427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RIAALLQT 8
           ++AA+LQT
Sbjct: 151 KVAAILQT 158
>NP_073741
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   RIAALLQTC 9
           R+A  LQ C
Sbjct: 596 RVAGRLQNC 604
>NP_001120926
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AALLQTC 9
           AALL TC
Sbjct: 237 AALLGTC 243
>NP_001120925
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AALLQTC 9
           AALL TC
Sbjct: 401 AALLGTC 407
>NP_006179
          Length = 109

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  IAALLQTC 9
          IAA LQ C
Sbjct: 12 IAAALQEC 19
>NP_004394
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   AALLQTC 9
           AALL TC
Sbjct: 401 AALLGTC 407
>NP_001091091
          Length = 109

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  IAALLQTC 9
          IAA LQ C
Sbjct: 12 IAAALQEC 19
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.341    0.137    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 353,745
Number of extensions: 440
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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