Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              21     229
NP_005602                                                              19     869
XP_005256140                                                           19     869
NP_620775                                                              18    1135
XP_005263337                                                           18    1135
NP_899662                                                              18    1935
NP_002886                                                              18    1935
XP_006723905                                                           18    1935
NP_006827                                                              17    2528
NP_683710                                                              17    4312
XP_006716207                                                           17    4312
NP_001138836                                                           17    4312
NP_683711                                                              17    4312
NP_055498                                                              16    5631
NP_002733                                                              16    5631
NP_055062                                                              16    5631
NP_001136082                                                           16    5631
XP_006721669                                                           16    5631
XP_005267916                                                           16    5631
XP_006714485                                                           16    5631
XP_005263418                                                           16    5631
XP_005263417                                                           16    5631
XP_006714484                                                           16    5631
XP_005263416                                                           16    5631
XP_006714483                                                           16    5631
XP_005263415                                                           16    5631
NP_005614                                                              16    7355
NP_055270                                                              15    9605
NP_056127                                                              15    9605
NP_663696                                                              15    9605
NP_653184                                                              15    9605
NP_001034451                                                           15    9605
NP_079380                                                              15    9605
XP_006722169                                                           15    9605
XP_006722168                                                           15    9605
XP_006722167                                                           15    9605
XP_006722166                                                           15    9605
XP_006722164                                                           15    9605
XP_006722163                                                           15    9605
XP_006722162                                                           15    9605
XP_006722161                                                           15    9605
XP_005254442                                                           15    9605
XP_006714407                                                           15    9605
XP_006714406                                                           15    9605
XP_005263347                                                           15    9605
XP_005263346                                                           15    9605
XP_005263345                                                           15    9605
XP_005263344                                                           15    9605
XP_005263343                                                           15    9605
XP_005263342                                                           15    9605
XP_005263341                                                           15    9605
XP_005263340                                                           15    9605
XP_005263339                                                           15    9605
XP_005273009                                                           15    9605
NP_002364                                                              15    9605
NP_000929                                                              15    9605
>NP_000312
          Length = 928

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RHLDQIMM 8
           RHLDQIMM
Sbjct: 698 RHLDQIMM 705
>NP_005602
          Length = 1139

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RHLDQIMM 8
           RHLDQ++M
Sbjct: 886 RHLDQLLM 893
>XP_005256140
          Length = 1065

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RHLDQIMM 8
           RHLDQ++M
Sbjct: 812 RHLDQLLM 819
>NP_620775
          Length = 409

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RHLDQIMMF 9
           RH DQIM F
Sbjct: 104 RHSDQIMTF 112
>XP_005263337
          Length = 360

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  RHLDQIMMF 9
          RH DQIM F
Sbjct: 55 RHSDQIMTF 63
>NP_899662
          Length = 1014

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RHLDQIMM 8
           RHLDQ+++
Sbjct: 838 RHLDQLLL 845
>NP_002886
          Length = 1068

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RHLDQIMM 8
           RHLDQ+++
Sbjct: 838 RHLDQLLL 845
>XP_006723905
          Length = 896

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RHLDQIMM 8
           RHLDQ+++
Sbjct: 666 RHLDQLLL 673
>NP_006827
          Length = 2671

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RHLDQIM 7
           RHLDQI+
Sbjct: 704 RHLDQII 710
>NP_683710
          Length = 315

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RHLDQIMMF 9
           +HLD+I+ F
Sbjct: 241 QHLDKILAF 249
>XP_006716207
          Length = 202

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RHLDQIMMF 9
           +HLD+I+ F
Sbjct: 128 QHLDKILAF 136
>NP_001138836
          Length = 258

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RHLDQIMMF 9
           +HLD+I+ F
Sbjct: 184 QHLDKILAF 192
>NP_683711
          Length = 308

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RHLDQIMMF 9
           +HLD+I+ F
Sbjct: 234 QHLDKILAF 242
>NP_055498
          Length = 1036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RHLDQIMMF 9
           RHL+ ++MF
Sbjct: 828 RHLNVMLMF 836
>NP_002733
          Length = 912

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HLDQIMM 8
           HLD+I+M
Sbjct: 258 HLDKILM 264
>NP_055062
          Length = 1499

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    HLDQIM 7
            H+DQIM
Sbjct: 1294 HMDQIM 1299
>NP_001136082
          Length = 1036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RHLDQIMMF 9
           RHL+ ++MF
Sbjct: 828 RHLNVMLMF 836
>XP_006721669
          Length = 1009

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RHLDQIMMF 9
           RHL+ ++MF
Sbjct: 801 RHLNVMLMF 809
>XP_005267916
          Length = 920

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HLDQIMM 8
           HLD+I+M
Sbjct: 266 HLDKILM 272
>XP_006714485
          Length = 1585

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    HLDQIM 7
            H+DQIM
Sbjct: 1380 HMDQIM 1385
>XP_005263418
          Length = 1654

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    HLDQIM 7
            H+DQIM
Sbjct: 1449 HMDQIM 1454
>XP_005263417
          Length = 1660

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    HLDQIM 7
            H+DQIM
Sbjct: 1455 HMDQIM 1460
>XP_006714484
          Length = 1685

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    HLDQIM 7
            H+DQIM
Sbjct: 1480 HMDQIM 1485
>XP_005263416
          Length = 1693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    HLDQIM 7
            H+DQIM
Sbjct: 1488 HMDQIM 1493
>XP_006714483
          Length = 1695

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    HLDQIM 7
            H+DQIM
Sbjct: 1490 HMDQIM 1495
>XP_005263415
          Length = 1701

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    HLDQIM 7
            H+DQIM
Sbjct: 1496 HMDQIM 1501
>NP_005614
          Length = 99

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  RHLDQI 6
          +HLDQI
Sbjct: 88 KHLDQI 93
>NP_055270
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2  HLDQIMMF 9
          HLD  M F
Sbjct: 77 HLDHTMFF 84
>NP_056127
          Length = 5005

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>NP_663696
          Length = 615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HLDQIMM 8
           H+D++MM
Sbjct: 183 HVDEMMM 189
>NP_653184
          Length = 381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   RHLDQI 6
           RHLD++
Sbjct: 340 RHLDEV 345
>NP_001034451
          Length = 427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   RHLDQI 6
           RHLD++
Sbjct: 386 RHLDEV 391
>NP_079380
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HLDQIMM 8
           H+D++MM
Sbjct: 183 HVDEMMM 189
>XP_006722169
          Length = 242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HLDQIMM 8
           H+D++MM
Sbjct: 183 HVDEMMM 189
>XP_006722168
          Length = 383

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HLDQIMM 8
           H+D++MM
Sbjct: 183 HVDEMMM 189
>XP_006722167
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HLDQIMM 8
           H+D++MM
Sbjct: 183 HVDEMMM 189
>XP_006722166
          Length = 573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  HLDQIMM 8
          H+D++MM
Sbjct: 62 HVDEMMM 68
>XP_006722164
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HLDQIMM 8
           H+D++MM
Sbjct: 183 HVDEMMM 189
>XP_006722163
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HLDQIMM 8
           H+D++MM
Sbjct: 162 HVDEMMM 168
>XP_006722162
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HLDQIMM 8
           H+D++MM
Sbjct: 167 HVDEMMM 173
>XP_006722161
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   HLDQIMM 8
           H+D++MM
Sbjct: 183 HVDEMMM 189
>XP_005254442
          Length = 3041

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   RHLDQI 6
           RHLD+I
Sbjct: 315 RHLDRI 320
>XP_006714407
          Length = 5004

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>XP_006714406
          Length = 3680

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>XP_005263347
          Length = 4640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>XP_005263346
          Length = 4983

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>XP_005263345
          Length = 4991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>XP_005263344
          Length = 5005

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>XP_005263343
          Length = 5009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>XP_005263342
          Length = 5025

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>XP_005263341
          Length = 5026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>XP_005263340
          Length = 5026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>XP_005263339
          Length = 5026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   HLDQIM 7
           HLDQ M
Sbjct: 242 HLDQFM 247
>XP_005273009
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2  HLDQIMMF 9
          HLD  M F
Sbjct: 44 HLDHTMFF 51
>NP_002364
          Length = 2803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  RHLDQI 6
          RHLD+I
Sbjct: 77 RHLDRI 82
>NP_000929
          Length = 1174

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   RHLDQI 6
           RH+DQ+
Sbjct: 638 RHIDQL 643
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.354    0.152    0.481 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 492,908
Number of extensions: 481
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)

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