Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              18    1135
NP_001138985                                                           17    4312
NP_055581                                                              17    4312
NP_005581                                                              17    4312
NP_001138987                                                           17    4312
NP_001138986                                                           17    4312
XP_005249975                                                           17    4312
NP_006218                                                              16    5631
NP_872617                                                              16    5631
NP_001229849                                                           16    5631
NP_689472                                                              16    5631
NP_001229850                                                           16    5631
XP_005260496                                                           16    5631
NP_061031                                                              16    7355
NP_057726                                                              16    7355
NP_001273483                                                           16    7355
NP_852607                                                              16    7355
NP_060733                                                              16    7355
XP_006721133                                                           16    7355
XP_005255502                                                           16    7355
XP_005255501                                                           16    7355
XP_005254495                                                           16    7355
XP_006715638                                                           16    7355
XP_005267216                                                           16    7355
XP_006712103                                                           16    7355
XP_006712102                                                           16    7355
XP_006710345                                                           16    7355
XP_006710344                                                           16    7355
XP_006710343                                                           16    7355
XP_006710342                                                           16    7355
XP_006710222                                                           16    7355
XP_006710221                                                           16    7355
XP_001717475                                                           16    7355
NP_001121773                                                           16    7355
NP_006305                                                              16    7355
NP_001121774                                                           16    7355
NP_065157                                                              16    7355
NP_115521                                                              16    7355
NP_001139241                                                           15    9605
NP_001161744                                                           15    9605
NP_004619                                                              15    9605
NP_115791                                                              15    9605
NP_699160                                                              15    9605
NP_588613                                                              15    9605
NP_001008738                                                           15    9605
XP_005252949                                                           15    9605
XP_005252948                                                           15    9605
XP_006715858                                                           15    9605
XP_006715857                                                           15    9605
XP_006713199                                                           15    9605
XP_006713198                                                           15    9605
XP_006713197                                                           15    9605
XP_006713196                                                           15    9605
XP_006713195                                                           15    9605
XP_005265166                                                           15    9605
XP_006713194                                                           15    9605
NP_848632                                                              15    9605
>NP_004251
          Length = 1208

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           RGRALRS L
Sbjct: 365 RGRALRSRL 373
>NP_001138985
          Length = 414

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           +GR LRST+
Sbjct: 364 QGRKLRSTM 372
>NP_055581
          Length = 414

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           +GR LRST+
Sbjct: 364 QGRKLRSTM 372
>NP_005581
          Length = 680

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RGRALRST 8
           RGRA +ST
Sbjct: 592 RGRAFKST 599
>NP_001138987
          Length = 346

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           +GR LRST+
Sbjct: 296 QGRKLRSTM 304
>NP_001138986
          Length = 434

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           +GR LRST+
Sbjct: 384 QGRKLRSTM 392
>XP_005249975
          Length = 414

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           +GR LRST+
Sbjct: 364 QGRKLRSTM 372
>NP_006218
          Length = 493

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           RG+ALR+ L
Sbjct: 380 RGKALRTQL 388
>NP_872617
          Length = 441

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           RG+ALR+ L
Sbjct: 328 RGKALRTQL 336
>NP_001229849
          Length = 398

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           RG+ALR+ L
Sbjct: 285 RGKALRTQL 293
>NP_689472
          Length = 1910

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RGRALRST 8
           RGR LRS+
Sbjct: 623 RGRTLRSS 630
>NP_001229850
          Length = 405

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           RG+ALR+ L
Sbjct: 292 RGKALRTQL 300
>XP_005260496
          Length = 513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           RG+ALR+ L
Sbjct: 400 RGKALRTQL 408
>NP_061031
          Length = 640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RGRALRS 7
           RGR LRS
Sbjct: 113 RGRTLRS 119
>NP_057726
          Length = 3674

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    RGRALRST 8
            RG ALR T
Sbjct: 1829 RGHALRDT 1836
>NP_001273483
          Length = 688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RGRALRS 7
           RGR LRS
Sbjct: 113 RGRTLRS 119
>NP_852607
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RGRALR 6
           RGRALR
Sbjct: 392 RGRALR 397
>NP_060733
          Length = 1435

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    RGRALRST 8
            RG+A+RS+
Sbjct: 1278 RGKAIRSS 1285
>XP_006721133
          Length = 122

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RGRALRS 7
           RGR LRS
Sbjct: 96  RGRGLRS 102
>XP_005255502
          Length = 122

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RGRALRS 7
           RGR LRS
Sbjct: 96  RGRGLRS 102
>XP_005255501
          Length = 122

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RGRALRS 7
           RGR LRS
Sbjct: 96  RGRGLRS 102
>XP_005254495
          Length = 3694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    RGRALRST 8
            RG ALR T
Sbjct: 1849 RGHALRDT 1856
>XP_006715638
          Length = 745

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GRALRSTL 9
           GR+LRS L
Sbjct: 734 GRSLRSVL 741
>XP_005267216
          Length = 745

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GRALRSTL 9
           GR+LRS L
Sbjct: 734 GRSLRSVL 741
>XP_006712103
          Length = 1175

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    RGRALRST 8
            RG+A+RS+
Sbjct: 1018 RGKAIRSS 1025
>XP_006712102
          Length = 1313

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    RGRALRST 8
            RG+A+RS+
Sbjct: 1156 RGKAIRSS 1163
>XP_006710345
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           R RAL+STL
Sbjct: 357 RVRALQSTL 365
>XP_006710344
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           R RAL+STL
Sbjct: 622 RVRALQSTL 630
>XP_006710343
          Length = 754

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           R RAL+STL
Sbjct: 656 RVRALQSTL 664
>XP_006710342
          Length = 761

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           R RAL+STL
Sbjct: 663 RVRALQSTL 671
>XP_006710222
          Length = 1793

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RGRALRSTL 9
           RG  LR+TL
Sbjct: 106 RGNLLRATL 114
>XP_006710221
          Length = 1793

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RGRALRSTL 9
           RG  LR+TL
Sbjct: 106 RGNLLRATL 114
>XP_001717475
          Length = 1826

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RGRALRSTL 9
           RG  LR+TL
Sbjct: 139 RGNLLRATL 147
>NP_001121773
          Length = 122

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RGRALRS 7
           RGR LRS
Sbjct: 96  RGRGLRS 102
>NP_006305
          Length = 713

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RGRALRSTL 9
           R RAL+STL
Sbjct: 615 RVRALQSTL 623
>NP_001121774
          Length = 122

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RGRALRS 7
           RGR LRS
Sbjct: 96  RGRGLRS 102
>NP_065157
          Length = 122

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RGRALRS 7
           RGR LRS
Sbjct: 96  RGRGLRS 102
>NP_115521
          Length = 745

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GRALRSTL 9
           GR+LRS L
Sbjct: 734 GRSLRSVL 741
>NP_001139241
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  RGRALRS 7
          RGR LRS
Sbjct: 12 RGRELRS 18
>NP_001161744
          Length = 2743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    RGRALRSTL 9
            RG ALR  L
Sbjct: 1725 RGEALRQVL 1733
>NP_004619
          Length = 940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  RGRALRS 7
          RGR LRS
Sbjct: 12 RGRELRS 18
>NP_115791
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALRSTL 9
           RALR+TL
Sbjct: 866 RALRNTL 872
>NP_699160
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GRALRSTL 9
           GRALR +L
Sbjct: 255 GRALRRSL 262
>NP_588613
          Length = 1166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GRALRST 8
           G+A+RST
Sbjct: 719 GKAMRST 725
>NP_001008738
          Length = 1138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GRALRST 8
           G+A+RST
Sbjct: 691 GKAMRST 697
>XP_005252949
          Length = 1001

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RGRALRSTL 9
          +GRALR  L
Sbjct: 38 QGRALRQRL 46
>XP_005252948
          Length = 1007

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RGRALRSTL 9
          +GRALR  L
Sbjct: 38 QGRALRQRL 46
>XP_006715858
          Length = 1267

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALRSTL 9
           RALR+TL
Sbjct: 979 RALRNTL 985
>XP_006715857
          Length = 1268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RALRSTL 9
           RALR+TL
Sbjct: 980 RALRNTL 986
>XP_006713199
          Length = 2695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    RGRALRSTL 9
            RG ALR  L
Sbjct: 1718 RGEALRQVL 1726
>XP_006713198
          Length = 2699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    RGRALRSTL 9
            RG ALR  L
Sbjct: 1718 RGEALRQVL 1726
>XP_006713197
          Length = 2712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    RGRALRSTL 9
            RG ALR  L
Sbjct: 1694 RGEALRQVL 1702
>XP_006713196
          Length = 2721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    RGRALRSTL 9
            RG ALR  L
Sbjct: 1703 RGEALRQVL 1711
>XP_006713195
          Length = 2727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    RGRALRSTL 9
            RG ALR  L
Sbjct: 1709 RGEALRQVL 1717
>XP_005265166
          Length = 2735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    RGRALRSTL 9
            RG ALR  L
Sbjct: 1717 RGEALRQVL 1725
>XP_006713194
          Length = 2736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    RGRALRSTL 9
            RG ALR  L
Sbjct: 1718 RGEALRQVL 1726
>NP_848632
          Length = 1039

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  RGRALRSTL 9
          +GRALR  L
Sbjct: 38 QGRALRQRL 46
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.137    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 345,045
Number of extensions: 399
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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