Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005276050                                                           17    2528
NP_077024                                                              17    4312
NP_631896                                                              16    5631
NP_001269475                                                           16    5631
NP_001269476                                                           16    5631
NP_005409                                                              16    5631
NP_114406                                                              16    5631
NP_998783                                                              16    5631
XP_005261298                                                           16    5631
XP_005261296                                                           16    5631
XP_005261295                                                           16    5631
XP_005253141                                                           16    5631
XP_005253140                                                           16    5631
XP_005253138                                                           16    5631
XP_005253137                                                           16    5631
XP_005253136                                                           16    5631
XP_006718348                                                           16    5631
XP_006718347                                                           16    5631
XP_005253134                                                           16    5631
NP_115948                                                              16    5631
NP_055386                                                              16    7355
NP_001257821                                                           16    7355
NP_001013757                                                           16    7355
XP_006726703                                                           16    7355
XP_006721421                                                           16    7355
XP_005270802                                                           16    7355
NP_001004480                                                           16    7355
NP_060250                                                              15    9605
NP_219501                                                              15    9605
NP_001026903                                                           15    9605
NP_072096                                                              15    9605
NP_775871                                                              15    9605
XP_006723058                                                           15    9605
XP_005257639                                                           15    9605
XP_006719261                                                           15    9605
XP_006716523                                                           15    9605
XP_006716522                                                           15    9605
XP_005251324                                                           15    9605
XP_005251323                                                           15    9605
XP_005250590                                                           15    9605
XP_006716151                                                           15    9605
XP_006716150                                                           15    9605
XP_006716149                                                           15    9605
XP_005250589                                                           15    9605
NP_006567                                                              15    9605
NP_001073903                                                           15    9605

>XP_005276050
          Length = 234

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGAGSTKAA 9
           RGAGST+ +
Sbjct: 177 RGAGSTRGS 185



>NP_077024
          Length = 678

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  GAGSTKAA 9
          GAG T+AA
Sbjct: 27 GAGKTRAA 34



>NP_631896
          Length = 717

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GAGSTKA 8
          GAG TKA
Sbjct: 14 GAGGTKA 20



>NP_001269475
          Length = 440

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  RGAGSTKA 8
          RGAG T A
Sbjct: 8  RGAGETSA 15



>NP_001269476
          Length = 435

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  RGAGSTKA 8
          RGAG T A
Sbjct: 8  RGAGETSA 15



>NP_005409
          Length = 1137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GAGSTKA 8
          GAG TKA
Sbjct: 14 GAGGTKA 20



>NP_114406
          Length = 435

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  RGAGSTKA 8
          RGAG T A
Sbjct: 8  RGAGETSA 15



>NP_998783
          Length = 1137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GAGSTKA 8
          GAG TKA
Sbjct: 14 GAGGTKA 20



>XP_005261298
          Length = 399

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RGAGSTKA 8
           RGAG+T A
Sbjct: 316 RGAGATPA 323



>XP_005261296
          Length = 416

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RGAGSTKA 8
           RGAG+T A
Sbjct: 333 RGAGATPA 340



>XP_005261295
          Length = 435

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RGAGSTKA 8
           RGAG+T A
Sbjct: 352 RGAGATPA 359



>XP_005253141
          Length = 717

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GAGSTKA 8
          GAG TKA
Sbjct: 14 GAGGTKA 20



>XP_005253140
          Length = 717

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GAGSTKA 8
          GAG TKA
Sbjct: 14 GAGGTKA 20



>XP_005253138
          Length = 850

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GAGSTKA 8
          GAG TKA
Sbjct: 44 GAGGTKA 50



>XP_005253137
          Length = 1137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GAGSTKA 8
          GAG TKA
Sbjct: 14 GAGGTKA 20



>XP_005253136
          Length = 1137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GAGSTKA 8
          GAG TKA
Sbjct: 14 GAGGTKA 20



>XP_006718348
          Length = 1137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GAGSTKA 8
          GAG TKA
Sbjct: 14 GAGGTKA 20



>XP_006718347
          Length = 1137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GAGSTKA 8
          GAG TKA
Sbjct: 14 GAGGTKA 20



>XP_005253134
          Length = 1167

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  GAGSTKA 8
          GAG TKA
Sbjct: 44 GAGGTKA 50



>NP_115948
          Length = 673

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RGAGSTKAA 9
           RGA +TKA+
Sbjct: 453 RGADTTKAS 461



>NP_055386
          Length = 328

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RGAGSTKAA 9
           RGA ST+ A
Sbjct: 240 RGASSTRRA 248



>NP_001257821
          Length = 127

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  AGSTKAA 9
          AG+TKAA
Sbjct: 38 AGATKAA 44



>NP_001013757
          Length = 432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AGSTKAA 9
           AGSTK+A
Sbjct: 121 AGSTKSA 127



>XP_006726703
          Length = 232

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RGAGSTKAA 9
          RG G  KAA
Sbjct: 47 RGVGGEKAA 55




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RGAGSTKAA 9
          RG G  KAA
Sbjct: 61 RGVGGEKAA 69




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RGAGSTKAA 9
          RG G  KAA
Sbjct: 75 RGVGGEKAA 83




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 117 RGVGGEKAA 125



>XP_006721421
          Length = 358

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RGAGSTKAA 9
          RG G  KAA
Sbjct: 33 RGVGGEKAA 41




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RGAGSTKAA 9
          RG G  KAA
Sbjct: 47 RGVGGEKAA 55




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RGAGSTKAA 9
          RG G  KAA
Sbjct: 61 RGVGGEKAA 69




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RGAGSTKAA 9
          RG G  KAA
Sbjct: 75 RGVGGEKAA 83




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  RGAGSTKAA 9
          RG G  KAA
Sbjct: 89 RGVGGEKAA 97




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 117 RGVGGEKAA 125




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 131 RGVGGEKAA 139




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 145 RGVGGEKAA 153




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 159 RGVGGEKAA 167




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 173 RGVGGEKAA 181




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 187 RGVGGEKAA 195




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 201 RGVGGEKAA 209




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 215 RGVGGEKAA 223




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 229 RGVGGEKAA 237




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 243 RGVGGEKAA 251




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 257 RGVGGEKAA 265




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 271 RGVGGEKAA 279




 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   RGAGSTKAA 9
           RG G  KAA
Sbjct: 285 RGVGGEKAA 293



>XP_005270802
          Length = 300

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RGAGSTKAA 9
           RGA ST+ A
Sbjct: 212 RGASSTRRA 220



>NP_001004480
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   GAGSTKA 8
           GAG TKA
Sbjct: 249 GAGRTKA 255



>NP_060250
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    RGAGSTKA 8
            RGAG+T +
Sbjct: 2135 RGAGNTSS 2142



>NP_219501
          Length = 196

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  RGAGSTK 7
          RG+GS+K
Sbjct: 28 RGSGSSK 34



>NP_001026903
          Length = 149

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  RGAGSTK 7
          RG+GS+K
Sbjct: 28 RGSGSSK 34



>NP_072096
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RGAGSTKA 8
           RG GST++
Sbjct: 116 RGRGSTRS 123



>NP_775871
          Length = 8384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    GAGSTKA 8
            G+GST+A
Sbjct: 7517 GSGSTRA 7523



>XP_006723058
          Length = 1460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RGAGSTKA 8
           RGAG T A
Sbjct: 615 RGAGETLA 622



>XP_005257639
          Length = 362

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RGAGSTK 7
           RG GST+
Sbjct: 328 RGVGSTR 334



>XP_006719261
          Length = 819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RGAGSTKA 8
           +G G+TKA
Sbjct: 296 KGKGATKA 303



>XP_006716523
          Length = 1912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    RGAGSTKA 8
            RGAG+T +
Sbjct: 1050 RGAGNTSS 1057



>XP_006716522
          Length = 2192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    RGAGSTKA 8
            RGAG+T +
Sbjct: 1330 RGAGNTSS 1337



>XP_005251324
          Length = 2996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    RGAGSTKA 8
            RGAG+T +
Sbjct: 2135 RGAGNTSS 2142



>XP_005251323
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    RGAGSTKA 8
            RGAG+T +
Sbjct: 2135 RGAGNTSS 2142



>XP_005250590
          Length = 1034

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RGAGSTKA 8
           RG GST++
Sbjct: 116 RGRGSTRS 123



>XP_006716151
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RGAGSTKA 8
           RG GST++
Sbjct: 116 RGRGSTRS 123



>XP_006716150
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RGAGSTKA 8
           RG GST++
Sbjct: 116 RGRGSTRS 123



>XP_006716149
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RGAGSTKA 8
           RG GST++
Sbjct: 116 RGRGSTRS 123



>XP_005250589
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RGAGSTKA 8
           RG GST++
Sbjct: 116 RGRGSTRS 123



>NP_006567
          Length = 819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RGAGSTKA 8
           +G G+TKA
Sbjct: 296 KGKGATKA 303



>NP_001073903
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   RGAGSTKA 8
           RGAG T A
Sbjct: 644 RGAGETLA 651



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.112    0.289 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 576,092
Number of extensions: 826
Number of successful extensions: 66
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 66
Number of HSP's successfully gapped: 66
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)