Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_872409                                                              18    1935
XP_006718595                                                           18    1935
NP_002200                                                              17    3301
NP_001107852                                                           17    3301
XP_006721107                                                           17    3301
XP_005255370                                                           17    3301
NP_653295                                                              17    4312
XP_005258622                                                           17    4312
NP_001114                                                              16    5631
NP_006712                                                              16    5631
NP_001189378                                                           16    5631
XP_005269595                                                           16    5631
NP_001186162                                                           16    7355
NP_001129490                                                           16    7355
NP_001278092                                                           16    7355
NP_000111                                                              16    7355
XP_006716983                                                           16    7355
NP_689549                                                              15    9605
NP_057314                                                              15    9605
NP_149079                                                              15    9605
NP_079076                                                              15    9605
NP_997698                                                              15    9605
NP_001257697                                                           15    9605
NP_665917                                                              15    9605
NP_653286                                                              15    9605
NP_001096078                                                           15    9605
NP_005188                                                              15    9605
NP_001597                                                              15    9605
NP_001139475                                                           15    9605
XP_006723099                                                           15    9605
XP_005256244                                                           15    9605
XP_006721357                                                           15    9605
XP_006721356                                                           15    9605
XP_006717059                                                           15    9605
XP_005248043                                                           15    9605
XP_006713958                                                           15    9605
XP_006713813                                                           15    9605
XP_006713812                                                           15    9605
XP_005247842                                                           15    9605
XP_005247840                                                           15    9605
XP_005247178                                                           15    9605
XP_006711058                                                           15    9605
XP_006711057                                                           15    9605
NP_065824                                                              15    9605
NP_001153685                                                           15    9605
NP_112583                                                              15    9605
NP_001078940                                                           15    9605
NP_001106832                                                           15    9605
>NP_872409
          Length = 389

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RFFKRNLMK 9
           RFFK+N+++
Sbjct: 210 RFFKQNILQ 218
>XP_006718595
          Length = 505

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RFFKRNLMK 9
           RFFK+N+++
Sbjct: 210 RFFKQNILQ 218
>NP_002200
          Length = 1170

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    FFKRNL 7
            FFKRNL
Sbjct: 1116 FFKRNL 1121
>NP_001107852
          Length = 1086

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    FFKRNL 7
            FFKRNL
Sbjct: 1032 FFKRNL 1037
>XP_006721107
          Length = 1087

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    FFKRNL 7
            FFKRNL
Sbjct: 1033 FFKRNL 1038
>XP_005255370
          Length = 1169

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    FFKRNL 7
            FFKRNL
Sbjct: 1115 FFKRNL 1120
>NP_653295
          Length = 536

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RFFKRNLM 8
           R FK+NLM
Sbjct: 197 RSFKKNLM 204
>XP_005258622
          Length = 592

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RFFKRNLM 8
           R FK+NLM
Sbjct: 253 RSFKKNLM 260
>NP_001114
          Length = 345

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RFFKRNLMK 9
           +F+K +LMK
Sbjct: 204 QFYKESLMK 212
>NP_006712
          Length = 362

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RFFKRNLMK 9
           +F+K +LMK
Sbjct: 221 QFYKESLMK 229
>NP_001189378
          Length = 327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RFFKRNLMK 9
           +F+K +LMK
Sbjct: 186 QFYKESLMK 194
>XP_005269595
          Length = 294

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RFFKRNLMK 9
           +F+K +LMK
Sbjct: 221 QFYKESLMK 229
>NP_001186162
          Length = 120

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FFKRNL 7
           F+KRNL
Sbjct: 97  FYKRNL 102
>NP_001129490
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RFFKRNL 7
           RF+K NL
Sbjct: 372 RFYKENL 378
>NP_001278092
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RFFKRNL 7
           RF+K NL
Sbjct: 372 RFYKENL 378
>NP_000111
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RFFKRNL 7
           RF+K NL
Sbjct: 372 RFYKENL 378
>XP_006716983
          Length = 173

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   FFKRNL 7
           F+KRNL
Sbjct: 150 FYKRNL 155
>NP_689549
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    FFKRNLM 8
            FFK NL+
Sbjct: 1051 FFKMNLL 1057
>NP_057314
          Length = 521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FFKRNLMK 9
           F KRNL K
Sbjct: 478 FLKRNLDK 485
>NP_149079
          Length = 837

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   RFFKRNLM 8
           +FF +N+M
Sbjct: 615 KFFNQNIM 622
>NP_079076
          Length = 645

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FFKRNLMK 9
           F K NL+K
Sbjct: 165 FLKNNLLK 172
>NP_997698
          Length = 2466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    RFFKRN 6
            RFF RN
Sbjct: 2328 RFFNRN 2333
>NP_001257697
          Length = 742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   RFFKRNLM 8
           +FF +N+M
Sbjct: 520 KFFNQNIM 527
>NP_665917
          Length = 470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FFKRNLMK 9
           F K NL+K
Sbjct: 165 FLKNNLLK 172
>NP_653286
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FFKRN 6
           FFKRN
Sbjct: 248 FFKRN 252
>NP_001096078
          Length = 2263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    RFFKRNLMK 9
            RF  RN+ K
Sbjct: 1847 RFISRNVFK 1855
>NP_005188
          Length = 468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FFKRN 6
           FFKRN
Sbjct: 239 FFKRN 243
>NP_001597
          Length = 2436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    RFFKRN 6
            RFF RN
Sbjct: 2298 RFFNRN 2303
>NP_001139475
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FFKRNLMK 9
           F K NL+K
Sbjct: 165 FLKNNLLK 172
>XP_006723099
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FFKRN 6
           FFKRN
Sbjct: 248 FFKRN 252
>XP_005256244
          Length = 807

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRNLMK 9
           KRN+MK
Sbjct: 343 KRNIMK 348
>XP_006721357
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRNLMK 9
           KRN+MK
Sbjct: 343 KRNIMK 348
>XP_006721356
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRNLMK 9
           KRN+MK
Sbjct: 343 KRNIMK 348
>XP_006717059
          Length = 2435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    RFFKRN 6
            RFF RN
Sbjct: 2297 RFFNRN 2302
>XP_005248043
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FFKRNLM 8
           FF RNL+
Sbjct: 238 FFARNLL 244
>XP_006713958
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FFKRNLM 8
           FF RNL+
Sbjct: 238 FFARNLL 244
>XP_006713813
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FFKRNLMK 9
           F K NL+K
Sbjct: 165 FLKNNLLK 172
>XP_006713812
          Length = 539

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  FFKRNLMK 9
          F K NL+K
Sbjct: 59 FLKNNLLK 66
>XP_005247842
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FFKRNLMK 9
           F K NL+K
Sbjct: 165 FLKNNLLK 172
>XP_005247840
          Length = 645

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FFKRNLMK 9
           F K NL+K
Sbjct: 165 FLKNNLLK 172
>XP_005247178
          Length = 2263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1    RFFKRNLMK 9
            RF  RN+ K
Sbjct: 1847 RFISRNVFK 1855
>XP_006711058
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   RFFKRNLM 8
           +FF +N+M
Sbjct: 309 KFFNQNIM 316
>XP_006711057
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   RFFKRNLM 8
           +FF +N+M
Sbjct: 616 KFFNQNIM 623
>NP_065824
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FFKRNLM 8
           FF RNL+
Sbjct: 238 FFARNLL 244
>NP_001153685
          Length = 882

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRNLMK 9
           KRN+MK
Sbjct: 198 KRNIMK 203
>NP_112583
          Length = 1007

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KRNLMK 9
           KRN+MK
Sbjct: 343 KRNIMK 348
>NP_001078940
          Length = 490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   FFKRN 6
           FFKRN
Sbjct: 239 FFKRN 243
>NP_001106832
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FFKRNLM 8
           FF RNL+
Sbjct: 238 FFARNLL 244
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.351    0.153    0.459 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 450,018
Number of extensions: 787
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.9 bits)
S2: 28 (15.4 bits)

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