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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              19     869
NP_036558                                                              18    1482
NP_001186623                                                           17    2528
NP_001186622                                                           17    2528
NP_001186621                                                           17    2528
NP_003031                                                              17    2528
NP_001277284                                                           17    4312
NP_001165376                                                           17    4312
NP_835227                                                              16    7355
NP_005188                                                              16    7355
NP_001078940                                                           16    7355
NP_940939                                                              15    9605
NP_001177257                                                           15    9605
NP_001108091                                                           15    9605
NP_001108092                                                           15    9605
NP_001108090                                                           15    9605
NP_002397                                                              15    9605
NP_001108089                                                           15    9605
NP_663780                                                              15    9605
NP_055674                                                              15    9605
NP_056101                                                              15    9605
NP_665804                                                              15    9605
NP_002193                                                              15    9605
NP_001027026                                                           15    9605
XP_005262533                                                           15    9605
XP_006722811                                                           15    9605
XP_005259960                                                           15    9605
XP_006721678                                                           15    9605
XP_005256948                                                           15    9605
XP_005256947                                                           15    9605
XP_005256946                                                           15    9605
XP_005256945                                                           15    9605
XP_006714930                                                           15    9605
XP_006714929                                                           15    9605
XP_005265973                                                           15    9605
XP_005265972                                                           15    9605
NP_079054                                                              15    9605

>NP_055365
          Length = 1032

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   REQMVEAAG 9
           REQ VEAAG
Sbjct: 648 REQRVEAAG 656



>NP_036558
          Length = 1217

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EQMVEAAG 9
           E+MVEAAG
Sbjct: 821 EEMVEAAG 828



>NP_001186623
          Length = 1227

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   QMVEAAG 9
           QMVEAAG
Sbjct: 648 QMVEAAG 654



>NP_001186622
          Length = 1232

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   QMVEAAG 9
           QMVEAAG
Sbjct: 653 QMVEAAG 659



>NP_001186621
          Length = 1241

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   QMVEAAG 9
           QMVEAAG
Sbjct: 662 QMVEAAG 668



>NP_003031
          Length = 1241

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   QMVEAAG 9
           QMVEAAG
Sbjct: 662 QMVEAAG 668



>NP_001277284
          Length = 1429

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    REQMVEAAG 9
            RE++V AAG
Sbjct: 1394 REELVAAAG 1402



>NP_001165376
          Length = 159

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   REQMVEAA 8
           REQ++EAA
Sbjct: 122 REQLLEAA 129



>NP_835227
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  REQMVEAA 8
          R+Q VEAA
Sbjct: 31 RDQQVEAA 38



>NP_005188
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EQMVEAAG 9
           E+M EAAG
Sbjct: 429 EEMKEAAG 436



>NP_001078940
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EQMVEAAG 9
           E+M EAAG
Sbjct: 451 EEMKEAAG 458



>NP_940939
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  REQMVEAAG 9
          R+Q+VE  G
Sbjct: 89 RQQLVELCG 97



>NP_001177257
          Length = 249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  REQMVEAAG 9
          R+Q+VE  G
Sbjct: 39 RQQLVELCG 47



>NP_001108091
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REQMVEAA 8
           +EQMV+A+
Sbjct: 246 KEQMVDAS 253



>NP_001108092
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REQMVEAA 8
           +EQMV+A+
Sbjct: 246 KEQMVDAS 253



>NP_001108090
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REQMVEAA 8
           +EQMV+A+
Sbjct: 246 KEQMVDAS 253



>NP_002397
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REQMVEAA 8
           +EQMV+A+
Sbjct: 246 KEQMVDAS 253



>NP_001108089
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REQMVEAA 8
           +EQMV+A+
Sbjct: 246 KEQMVDAS 253



>NP_663780
          Length = 1565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   REQMVEAAG 9
           R+Q+VE  G
Sbjct: 946 RQQLVEVIG 954



>NP_055674
          Length = 818

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REQMVEAA 8
           RE+M EAA
Sbjct: 180 REEMEEAA 187



>NP_056101
          Length = 1253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   REQMVEAAG 9
           R+Q+VE  G
Sbjct: 946 RQQLVEVIG 954



>NP_665804
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   REQMVEAAG 9
           +EQ+VE  G
Sbjct: 221 KEQLVEMTG 229



>NP_002193
          Length = 349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   REQMVEAAG 9
           +EQ+VE  G
Sbjct: 211 KEQLVEMTG 219



>NP_001027026
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REQMVEAA 8
           RE+ VEAA
Sbjct: 255 RERQVEAA 262



>XP_005262533
          Length = 811

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REQMVEAA 8
           RE+ VEAA
Sbjct: 255 RERQVEAA 262



>XP_006722811
          Length = 335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   REQMVEAAG 9
           R+Q+VE  G
Sbjct: 162 RQQLVELCG 170



>XP_005259960
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   REQMVEAAG 9
           R+Q+VE  G
Sbjct: 162 RQQLVELCG 170



>XP_006721678
          Length = 692

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REQMVEAA 8
           RE+M EAA
Sbjct: 180 REEMEEAA 187



>XP_005256948
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REQMVEAA 8
           RE+M EAA
Sbjct: 180 REEMEEAA 187



>XP_005256947
          Length = 768

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REQMVEAA 8
           RE+M EAA
Sbjct: 180 REEMEEAA 187



>XP_005256946
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REQMVEAA 8
           RE+M EAA
Sbjct: 180 REEMEEAA 187



>XP_005256945
          Length = 812

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REQMVEAA 8
           RE+M EAA
Sbjct: 180 REEMEEAA 187



>XP_006714930
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REQMVEAA 8
           +EQMV+A+
Sbjct: 246 KEQMVDAS 253



>XP_006714929
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REQMVEAA 8
           +EQMV+A+
Sbjct: 246 KEQMVDAS 253



>XP_005265973
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REQMVEAA 8
           +EQMV+A+
Sbjct: 246 KEQMVDAS 253



>XP_005265972
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REQMVEAA 8
           +EQMV+A+
Sbjct: 246 KEQMVDAS 253



>NP_079054
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REQMVEAA 8
           RE+ VEAA
Sbjct: 255 RERQVEAA 262



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.119    0.292 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,945
Number of extensions: 870
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)