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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              19     869
NP_005913                                                              19     869
XP_005267046                                                           19     869
XP_005267045                                                           19     869
NP_945120                                                              18    1482
NP_002378                                                              18    1935
NP_001078846                                                           18    1935
NP_061972                                                              18    1935
XP_005272048                                                           18    1935
XP_006714680                                                           18    1935
NP_667338                                                              17    3301
NP_001159608                                                           17    3301
XP_006713923                                                           17    3301
XP_006713922                                                           17    3301
NP_619729                                                              17    4312
NP_619730                                                              17    4312
NP_002436                                                              17    4312
NP_006819                                                              16    7355
XP_006715385                                                           16    7355
XP_006713065                                                           16    7355
XP_005264953                                                           16    7355
XP_006713064                                                           16    7355
XP_006713063                                                           16    7355
XP_006713062                                                           16    7355
XP_006713061                                                           16    7355
XP_006713060                                                           16    7355
XP_006713059                                                           16    7355
XP_006713058                                                           16    7355
XP_006713057                                                           16    7355
NP_955379                                                              16    7355
NP_001191355                                                           15    9605
NP_878314                                                              15    9605
NP_002305                                                              15    9605

>NP_006715
          Length = 1558

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ELMAMKEI 9
            ELMAMKEI
Sbjct: 1317 ELMAMKEI 1324



>NP_005913
          Length = 1608

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ELMAMKEI 9
            ELMAMKEI
Sbjct: 1367 ELMAMKEI 1374



>XP_005267046
          Length = 1554

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ELMAMKEI 9
            ELMAMKEI
Sbjct: 1313 ELMAMKEI 1320



>XP_005267045
          Length = 1604

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    ELMAMKEI 9
            ELMAMKEI
Sbjct: 1363 ELMAMKEI 1370



>NP_945120
          Length = 282

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  RELMAMKEI 9
          R+LM+MKE+
Sbjct: 2  RQLMSMKEV 10



>NP_002378
          Length = 829

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RELMAMKE 8
           +ELMAMKE
Sbjct: 620 QELMAMKE 627



>NP_001078846
          Length = 1019

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RELMAMKE 8
           +ELMAMKE
Sbjct: 810 QELMAMKE 817



>NP_061972
          Length = 297

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  RELMAMKEI 9
          +ELM+MKE+
Sbjct: 17 QELMSMKEV 25



>XP_005272048
          Length = 851

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RELMAMKE 8
           +ELMAMKE
Sbjct: 620 QELMAMKE 627



>XP_006714680
          Length = 1002

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   RELMAMKE 8
           +ELMAMKE
Sbjct: 771 QELMAMKE 778



>NP_667338
          Length = 1308

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RELMAMKE 8
           RE+MAM+E
Sbjct: 472 REMMAMEE 479



>NP_001159608
          Length = 1228

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RELMAMKE 8
           RE+MAM+E
Sbjct: 392 REMMAMEE 399



>XP_006713923
          Length = 1228

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RELMAMKE 8
           RE+MAM+E
Sbjct: 392 REMMAMEE 399



>XP_006713922
          Length = 1262

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RELMAMKE 8
           RE+MAM+E
Sbjct: 472 REMMAMEE 479



>NP_619729
          Length = 451

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ELMAMKE 8
           E+MAMKE
Sbjct: 225 EIMAMKE 231



>NP_619730
          Length = 388

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ELMAMKE 8
           E+MAMKE
Sbjct: 225 EIMAMKE 231



>NP_002436
          Length = 358

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ELMAMKE 8
           E+MAMKE
Sbjct: 225 EIMAMKE 231



>NP_006819
          Length = 2202

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RELMAMKEI 9
            REL+A+K +
Sbjct: 2125 RELIALKRV 2133



>XP_006715385
          Length = 1866

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RELMAMKEI 9
            REL+A+K +
Sbjct: 1789 RELIALKRV 1797



>XP_006713065
          Length = 709

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RELMAMKEI 9
           + L+AMKE+
Sbjct: 542 QNLLAMKEV 550



>XP_005264953
          Length = 713

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RELMAMKEI 9
           + L+AMKE+
Sbjct: 542 QNLLAMKEV 550



>XP_006713064
          Length = 1115

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RELMAMKEI 9
           + L+AMKE+
Sbjct: 542 QNLLAMKEV 550



>XP_006713063
          Length = 1125

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RELMAMKEI 9
           + L+AMKE+
Sbjct: 542 QNLLAMKEV 550



>XP_006713062
          Length = 1158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RELMAMKEI 9
           + L+AMKE+
Sbjct: 542 QNLLAMKEV 550



>XP_006713061
          Length = 1162

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RELMAMKEI 9
           + L+AMKE+
Sbjct: 542 QNLLAMKEV 550



>XP_006713060
          Length = 1162

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RELMAMKEI 9
           + L+AMKE+
Sbjct: 542 QNLLAMKEV 550



>XP_006713059
          Length = 1172

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RELMAMKEI 9
           + L+AMKE+
Sbjct: 542 QNLLAMKEV 550



>XP_006713058
          Length = 1172

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RELMAMKEI 9
           + L+AMKE+
Sbjct: 542 QNLLAMKEV 550



>XP_006713057
          Length = 1172

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RELMAMKEI 9
           + L+AMKE+
Sbjct: 542 QNLLAMKEV 550



>NP_955379
          Length = 712

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RELMAMKEI 9
           + L+AMKE+
Sbjct: 542 QNLLAMKEV 550



>NP_001191355
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ELMAMKEI 9
           E+M MKE+
Sbjct: 329 EVMVMKEL 336



>NP_878314
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RELMAMK 7
           RE++AMK
Sbjct: 178 REMLAMK 184



>NP_002305
          Length = 647

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ELMAMKEI 9
           E+M MKE+
Sbjct: 363 EVMVMKEL 370



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.133    0.313 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 384,021
Number of extensions: 863
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)