Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     665
NP_001075109                                                           19     665
NP_000673                                                              17    3301
NP_000672                                                              17    3301
NP_000674                                                              17    3301
NP_006853                                                              17    4312
NP_001077434                                                           17    4312
NP_001077433                                                           17    4312
NP_001077432                                                           17    4312
XP_005244911                                                           17    4312
XP_005244910                                                           17    4312
NP_115977                                                              16    5631
NP_477520                                                              16    5631
NP_001139266                                                           16    5631
NP_775817                                                              16    5631
NP_001129477                                                           16    5631
NP_001129478                                                           16    5631
NP_000810                                                              16    5631
XP_006724053                                                           16    5631
XP_006724052                                                           16    5631
XP_005260998                                                           16    5631
XP_006724051                                                           16    5631
XP_005264997                                                           16    5631
XP_005264996                                                           16    5631
XP_005264995                                                           16    5631
XP_006713079                                                           16    5631
XP_006713078                                                           16    5631
NP_780294                                                              16    5631
NP_004689                                                              16    7355
NP_068711                                                              16    7355
NP_000804                                                              16    7355
NP_001005853                                                           16    7355
XP_005247061                                                           16    7355
NP_775486                                                              16    7355
NP_001191355                                                           15    9605
NP_001009899                                                           15    9605
NP_036385                                                              15    9605
NP_001139406                                                           15    9605
NP_001139405                                                           15    9605
NP_777577                                                              15    9605
NP_001230208                                                           15    9605
NP_001139373                                                           15    9605
NP_060297                                                              15    9605
NP_001093122                                                           15    9605
NP_001120686                                                           15    9605
NP_002305                                                              15    9605
XP_006721229                                                           15    9605
XP_005256350                                                           15    9605
XP_005247266                                                           15    9605
XP_005247265                                                           15    9605
NP_001139669                                                           15    9605
NP_653176                                                              15    9605
>NP_008835
          Length = 4128

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   REKRLSFVV 9
           REKRLSF V
Sbjct: 888 REKRLSFAV 896
>NP_001075109
          Length = 4097

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   REKRLSFVV 9
           REKRLSF V
Sbjct: 888 REKRLSFAV 896
>NP_000673
          Length = 450

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   REKRLSFVV 9
           REKR +FV+
Sbjct: 365 REKRFTFVL 373
>NP_000672
          Length = 465

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   REKRLSFVV 9
           REKR +FV+
Sbjct: 383 REKRFTFVL 391
>NP_000674
          Length = 462

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   REKRLSFVV 9
           REKR +FV+
Sbjct: 376 REKRFTFVL 384
>NP_006853
          Length = 561

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  REKRLSFV 8
          RE+RL+FV
Sbjct: 41 REERLTFV 48
>NP_001077434
          Length = 561

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  REKRLSFV 8
          RE+RL+FV
Sbjct: 41 REERLTFV 48
>NP_001077433
          Length = 516

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  REKRLSFV 8
          RE+RL+FV
Sbjct: 41 REERLTFV 48
>NP_001077432
          Length = 561

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  REKRLSFV 8
          RE+RL+FV
Sbjct: 41 REERLTFV 48
>XP_005244911
          Length = 561

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  REKRLSFV 8
          RE+RL+FV
Sbjct: 41 REERLTFV 48
>XP_005244910
          Length = 561

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  REKRLSFV 8
          RE+RL+FV
Sbjct: 41 REERLTFV 48
>NP_115977
          Length = 353

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REKRLSFV 8
           +EK+LSF+
Sbjct: 235 QEKKLSFI 242
>NP_477520
          Length = 488

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EKRLSFVV 9
          E RLSF+V
Sbjct: 77 EMRLSFIV 84
>NP_001139266
          Length = 489

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EKRLSFVV 9
          E RLSF+V
Sbjct: 77 EMRLSFIV 84
>NP_775817
          Length = 354

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REKRLSFVV 9
           R+KR  FVV
Sbjct: 268 RDKRADFVV 276
>NP_001129477
          Length = 1010

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  REKRLSFVV 9
          +EK++ FVV
Sbjct: 63 KEKKIEFVV 71
>NP_001129478
          Length = 1010

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  REKRLSFVV 9
          +EK++ FVV
Sbjct: 63 KEKKIEFVV 71
>NP_000810
          Length = 1010

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  REKRLSFVV 9
          +EK++ FVV
Sbjct: 63 KEKKIEFVV 71
>XP_006724053
          Length = 1010

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  REKRLSFVV 9
          +EK++ FVV
Sbjct: 63 KEKKIEFVV 71
>XP_006724052
          Length = 1010

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  REKRLSFVV 9
          +EK++ FVV
Sbjct: 63 KEKKIEFVV 71
>XP_005260998
          Length = 1010

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  REKRLSFVV 9
          +EK++ FVV
Sbjct: 63 KEKKIEFVV 71
>XP_006724051
          Length = 1026

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  REKRLSFVV 9
          +EK++ FVV
Sbjct: 79 KEKKIEFVV 87
>XP_005264997
          Length = 227

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REKRLSFVV 9
           R+KR  FVV
Sbjct: 141 RDKRADFVV 149
>XP_005264996
          Length = 234

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REKRLSFVV 9
           R+KR  FVV
Sbjct: 148 RDKRADFVV 156
>XP_005264995
          Length = 361

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REKRLSFVV 9
           R+KR  FVV
Sbjct: 275 RDKRADFVV 283
>XP_006713079
          Length = 364

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REKRLSFVV 9
           R+KR  FVV
Sbjct: 278 RDKRADFVV 286
>XP_006713078
          Length = 371

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REKRLSFVV 9
           R+KR  FVV
Sbjct: 285 RDKRADFVV 293
>NP_780294
          Length = 433

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  REKRLSFVV 9
          +EK++ FVV
Sbjct: 63 KEKKIEFVV 71
>NP_004689
          Length = 683

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REKRLSFV 8
           R+K+LSF+
Sbjct: 156 RKKQLSFI 163
>NP_068711
          Length = 512

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   REKRLSFVV 9
           R+KRLS+ V
Sbjct: 92  RDKRLSYNV 100
>NP_000804
          Length = 474

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   REKRLSFVV 9
           R+KRLS+ V
Sbjct: 92  RDKRLSYNV 100
>NP_001005853
          Length = 312

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  REKRLSFV 8
          ++KR+SFV
Sbjct: 88 QQKRISFV 95
>XP_005247061
          Length = 312

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  REKRLSFV 8
          ++KR+SFV
Sbjct: 88 QQKRISFV 95
>NP_775486
          Length = 331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  REKRLSFV 8
          ++KR+SFV
Sbjct: 88 QQKRISFV 95
>NP_001191355
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   REKRLSFV 8
           ++KRL+F+
Sbjct: 371 KDKRLNFI 378
>NP_001009899
          Length = 2245

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    REKRLSF 7
            R+KRLS+
Sbjct: 1788 RQKRLSY 1794
>NP_036385
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  KRLSFV 8
          KRLSFV
Sbjct: 84 KRLSFV 89
>NP_001139406
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  KRLSFV 8
          KRLSFV
Sbjct: 28 KRLSFV 33
>NP_001139405
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  KRLSFV 8
          KRLSFV
Sbjct: 78 KRLSFV 83
>NP_777577
          Length = 576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REKRLSFV 8
           RE+R+SF+
Sbjct: 94  REERVSFL 101
>NP_001230208
          Length = 576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REKRLSFV 8
           RE+R+SF+
Sbjct: 94  REERVSFL 101
>NP_001139373
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REKRLSFV 8
           R+KRLS V
Sbjct: 218 RQKRLSHV 225
>NP_060297
          Length = 518

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REKRLSFV 8
           R+KRLS V
Sbjct: 216 RQKRLSHV 223
>NP_001093122
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  REKRLSF 7
          RE+RLS+
Sbjct: 75 RERRLSY 81
>NP_001120686
          Length = 576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REKRLSFV 8
           RE+R+SF+
Sbjct: 94  REERVSFL 101
>NP_002305
          Length = 647

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   REKRLSFV 8
           ++KRL+F+
Sbjct: 405 KDKRLNFI 412
>XP_006721229
          Length = 427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REKRLSFV 8
           RE+R+SF+
Sbjct: 94  REERVSFL 101
>XP_005256350
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   REKRLSFV 8
           RE+R+SF+
Sbjct: 94  REERVSFL 101
>XP_005247266
          Length = 2189

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    REKRLSF 7
            R+KRLS+
Sbjct: 1732 RQKRLSY 1738
>XP_005247265
          Length = 2211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    REKRLSF 7
            R+KRLS+
Sbjct: 1754 RQKRLSY 1760
>NP_001139669
          Length = 7081

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    REKRLS 6
            REKRLS
Sbjct: 1780 REKRLS 1785
>NP_653176
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  REKRLSFVV 9
          +EKRLS V+
Sbjct: 53 QEKRLSNVI 61
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.144    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 508,771
Number of extensions: 702
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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